Strain Information
| Name | RG3562 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | pars-2(ve1062[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP])/hIn1[umnIs78 unc-54(h1040)] I. |
| Description | umnIs78 [myo-2p::mKate2 + NeoR, I: 12541645 (intergenic)] I. Sterile. Deletion of 3099 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Heterozygotes are wild-type GFP+ mKate2+, and segregate wild-type GFP+ mKate2+, sterile GFP+ non-mKate2 (ve1062 homozygotes), and Unc non-GFP mKate2+ animals (hIn1[umnIs78 unc-54(h1040)] homozygotes). Maintain by picking wild-type GFP+mKate2+. Left flanking Sequence: aaaattgatgtaaaaatcgtgaaaatcaca; Right flanking sequence: CGGAAAGCGAATTCACGACGCAAATCGACT. pars-2 crRNA A: agaaattgtcctgaaagctg; pars-2 crRNA B: GATCGTGTGGAAATGTCGGC. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation. |
| Mutagen | Crispr/Cas9 |
| Made by | RG KO Group |
| Laboratory | RG |
| Reference | n/a |
Sign in
or
register an account if you want to order this strain.