Strain Information
| Name | DV3709 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | ieSi57 II; unc-119(ed3) III; mig-15(re264[AID*::mNeonGreen::2xHA::mig-15]) X. |
| Description | ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. AID*::mNeonGreen::2xHA tag inserted into endogenous mig-15 locus. Single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. This strain can be used for auxin-inducible degradation (AID) of MIG-15 in somatic tissues. Auxin treatment causes locomotion defects, pVul, and weak Muv. Ubiquitous red fluorescence in cytoplasm. Ubiquitous mNeonGreen expression, some cytoplasmic, some localized to punctae and junctions. References: Huynh L, et al. BioRxiv. 2025 Aug 29:2025.08.22.671798. doi: 10.1101/2025.08.22.671798. PMID: 40909657. Fakieh RA & Reiner DJ. Proc Natl Acad Sci USA. 2025 Jan 7;122(1):e2414321121. doi: 10.1073/pnas.2414321121. PMID: 39739816. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x1 |
| Made by | Razan Fakieh |
| Laboratory | DV |
| Reference | Huynh, Fakieh, Hendrix, Powell, Reiner BioRxiv (2025) PMID: 40909657 Will likely be in press in early 2026 See also Fakieh and Reiner (2025) PMID: 39739816 |
Sign in
or
register an account if you want to order this strain.