Strain Information
| Name | DV3651 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | his-72(erb77[his-72::linker::mTurquoise2]) III; yap-1(re269[yap-1::mNeonGreen::2xFLAG]) X. |
| Description | mTurqoise2 tag with linker sequence inserted into endogenous his-72 locus. mNeonGreen::2xFLAG tag inserted into endogenous yap-1 locus. Ubiquitous yellow fluorescence (488 nm or 514 nm) is cytosolic with modest nuclear signal and strong exclusion from nucleoli, but translocates to nuclei upon disruption of upstream Hippo pathway components. Deficiency of cst-1/2 (Hippo/MST) causes translocation into nuclei of all hypodermal cells, deficiency of wts-1 (Warts/LATS) causes translocation into nuclei of all cell types observed. YAP-1::mNG::2xFLAG may be non-functional, as endogenous tag blocks lethality conferred by deficiency of WTS-1. References: Huynh L, et al. BioRxiv. 2025 Aug 29:2025.08.22.671798. doi: 10.1101/2025.08.22.671798. PMID: 40909657. Sloan DE & Bembenek JN. MicroPubl Biol. 2021 Sep 29:2021:10.17912/micropub.biology.000471. PMID: 34604717. |
| Mutagen | CRISPR |
| Outcrossed | x1 |
| Made by | Razan Fakieh |
| Laboratory | DV |
| Reference | for yap-1: Huynh, Fakieh, Hendrix, Powell, Reiner, BioRxiv (2025) PMID: 40909657. Expected to be published in early 2026. for his-72: Sloan and Bembenek, Micropublication Biology (2021) PMID: 34604717 |
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