Species Information: Caenorhabditis elegans

Name Caenorhabditis elegans
NCBI Taxonomy ID 6239

Caenorhabditis elegans strains available at the CGC

Strain Genotype Description
AR20 suls-1(mr35) V. Sensitive to hydrogen sulfide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32.
AR21 suls-2(mr36) I. Sensitive to hydrogen sulfide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32.
AR23 suls-1(mr38) V. Sensitive to hydrogen sulfide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32.
AR24 cysl-1(mr39) X. Sensitive to hydrogen sulfide and hydrogen cyanide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32.
AR25 cysl-1(mr40) X. Sensitive to hydrogen sulfide and hydrogen cyanide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32.
AS270 gly-12(id47) X. Phenotypically WT. F3.2 See also WBPaper00005927.
AS271 gly-14(id48) III. Phenotypically WT. M8ns. See also WBPaper00005927.
AS338 dpy-6(e14) gly-13(ok712) X.
AT6 srf-2(yj262) I. Superficially wild-type. Antibody staining required to observe phenotype.
AT10 srf-3(yj10) IV. Superficially wild-type. Antibody staining required to observe phenotype. Contains at least one extraneous mutation in the background (Eur. Worm Mtg 2006, Venuz et al.).
AU1 sek-1(ag1) X. Enhanced susceptibility to pathogens (Esp). Egl-d. Nsy. Also called esp-2.
AU3 nsy-1(ag3) II. Enhanced susceptibility to pathogens (Esp). Nsy. Egl-d. Also called esp-8.
AU37 glp-4(bn2) I; sek-1(km4) X. Temperature sensitive sterility. Maintain at 15C. Enhanced sensitivity to pathogens.
AU98 inx-14(ag17) I. Enhanced pathogen resistance.
AU147 daf-16(mgDf47) I; glp-1(e2141) III. Temperature sensitive sterility. Maintain at 15C.
AU166 daf-16(mgDf47) I; fog-2(q71) V. Temperature sensitive. Maintain at 15C.
AV38 mnDp66 (X;I); meDf2 X. Produces 31% XO male self progeny; nondisjunction is correlated with a high frequency of achiasmate X chromosomes in oocyte nuclei, and a reduced frequency of X chromosome crossovers. meDf2 disrupts the function of the cis-acting X chromosome meiotic pairing center. meDf2/+ heterozygotes produce 4-6% XO progeny, so the presence of meDf2 can be followed in heterozygotes by this weak Him phenotype.
AV39 mnDp66 (X;I); meDf3 X. Produces 32% XO male self progeny; nondisjunction is correlated with a high frequency of achiasmate X chromosomes in oocyte nuclei, and a reduced frequency of X chromosome crossovers. meDf3 disrupts the function of the cis-acting X chromosome meiotic pairing center. meDf3/+ heterozygotes produce 4-6% XO progeny, so the presence of meDf3 can be followed in heterozygotes by this weak Him phenotype.
AV40 mnDp66 (X;I); meDf4 X. Produces 27% XO male self progeny; nondisjunction is correlated with a high frequency of achiasmate X chromosomes in oocyte nuclei, and a reduced frequency of X chromosome crossovers. meDf4 disrupts the function of the cis-acting X chromosome meiotic pairing center. meDf4/+ heterozygotes produce 4-6% XO progeny, so the presence of meDf4 can be followed in heterozygotes by this weak Him phenotype.
AV41 mnDp66 (X;I); meDf5 X. Produces 32% XO male self-progeny; nondisjunction is correlated with a high frequency of achiasmate X chromosomes in oocyte nuclei, and a reduced frequency of X chromosome crossovers. meDf5 disrupts the function of the cis-acting X chromosome meiotic pairing center. meDf5/+ heterozygotes produce 4-6% XO progeny, so the presence of meDf5 can be followed in heterozygotes by this weak Him phenotype.
AV51 me8 X. Homozygotes produce 10-15% XO male self progeny; nondisjuction is correlated with an increased frequency of achiasmate X chromosomes in oocyte nuclei, and an unaltered distribution of X chromosome crossovers. Heterozygotes produce 1-2% male self-progeny. Homozygotes (and XO hemizygotes) are slower growing than WT; reduced male mating efficiency. me8 disrupts the function of the cis-acting X chromosome meiotic pairing center. Molecular studies show that the me8 chromosome carries a terminal deletion that removes >70 kb from the left end of the X chromosome, including the endogenous telomere; further, a segment of chromosome V has been translocated to the left end of X, and a new telomere has been added de novo to the end of the translocated segment.
AV106 spo-11(ok79) IV/nT1 [unc-?(n754) let-?] (IV;V). Heterozygotes are Unc and segregate Uncs (heterozygotes), non-Unc spo-11 homozygotes, and dead eggs (nT1 homozygotes). spo-11 homozygotes produce an average of ~200 fertilized eggs but only about 0.1 progeny survive to adulthood. When mated to N2 males, spo-11 homozygotes will produce at least 5-10 cross progeny.
AV112 mre-11(ok179) IV/nT1 [unc-?(n754) let-?] (IV;V). Heterozygotes are Unc and segregate Uncs (heterozygotes), non-Unc mre-11 homozygotes, and dead eggs (nT1 homozygotes). mre-11 homozygotes produce about 200 fertilized eggs but only about 2-3% of these eggs survive to adulthood (this mutation cannot be maintained in a homozygous condition). Occasionally non-Unc progeny that do not demonstrate the mre-11(ok179) mutant phenotype arise when grown in large liquid cultures. mre-11 is the predicted gene ZC302.1
AV115 msh-5(me23) IV/nT1 [unc-?(n754) let-?] (IV;V). Heterozygotes are Unc and segregate Uncs (heterozygotes), non-Unc msh-5 homozygotes, and dead eggs (nT1 homozygotes). msh-5 homozygotes give 97.9% dead eggs; of those that hatch, 42% are male.
AV125 spe-8(hc40) I; dpy-4(e1166) IV. Can be maintained by chunking or setting up male/hermaphrodite crosses. Dpy.
AV146 chk-2(me64) rol-9(sc148)/unc-51(e369) rol-9(sc148) V. Heterozygotes are fertile Rollers and segregate fertile non-Rollers (heterozygote), Unc Rollers (unc-51 homozygotes), and non-Unc Rollers that give 96-97% dead eggs (a high % of the survivors are males).
AV157 spo-11(me44)/nT1 [unc-?(n754) let-? qIs50] (IV;V). Balanced heterozygotes are GFP+ Unc and segregate GFP+ Unc (heterozygotes), non-GFP non-Unc spo-11(me44) homozygotes, and dead eggs (nT1 homozygotes). spo-11(me44) homozygotes are viable and produce more than 90% dead eggs (a large fraction of the survivors are males — strong Him phenotype); cytologically they lack chiasmata in diakinesis-stage oocytes and lack RAD-51 foci. Maintain by picking Unc.
AV158 rad-50(ok197)/nT1[unc-?(n754) let-? qIs50] (IV;V). Balanced heterozygotes are GFP+ Unc and segregate GFP+ Unc (heterozygotes), non-GFP non-Unc rad-50(ok197) homozygotes, and dead eggs (nT1 homozygotes). rad-50(ok197) homozygotes are viable and produce more than 98.8% dead eggs (a large fraction of the survivors are males — strong Him phenotype); cytologically they lack chiasmata in diakinesis-stage oocytes, but pairing and synapsis appear normal. Maintain by picking Unc.
AV267 syp-3(me42)/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Heterozygotes are WT and GFP+. Segregate syp-3(me42) homozygotes that are non-GFP and lay mostly dead eggs; these mutants form abnormal synaptonemal complex formation during meiosis. Homozygous hT2[bli-4 let-? qIs48] animals are inviable.
AV271 him-3(me80)/nT1 [unc-?(n754) let-? qIs50] (IV;V). Balanced heterozygotes are GFP+ Unc and segregate GFP+ Unc (heterozygotes), non-GFP non-Unc him-3(me80) homozygotes, and dead eggs (nT1 homozygotes). him-3(me80) homozygotes are viable and non-Unc. They produce more than 85% dead eggs and a large fraction (11%) of the survivors are males (Him phenotype). Cytologically they exhibit a reduced level of HIM-3 loading and fewer stretches of SYP-1 than WT. In diakinesis-stage oocytes, they contain a mixture of bivalents and univalents. Maintain by picking Unc.
AV276 syp-2(ok307) V/nT1 [unc-?(n754) let-?(m435)] (IV;V). Balanced heterozygotes are Unc and segregate Unc (heterozygotes), non-Unc syp-2(ok307) homozygotes, and dead eggs (nT1 homozygotes). syp-2(ok307) homozygotes are viable and non-Unc. They produce 96% dead eggs and 44% males; cytologically they lack chiasmata in diakinesis-stage oocytes, exhibit persistent polarized nuclear organization during earlier meiotic prophase, lack synaptonemal complexes, and exhibit unstable pairing of homologous chromosomes.
AV280 unc-119(e2498) III; him-17(ok424) V; meIs5. meIs5 [him-17::GFP + unc-119(+)]. him-17::GFP is expressed in the germline. meIs5 not mapped.
AV285 swm-1(me66) him-5(e1490) V. Sperm activation in virgin males. Poor sperm transfer.
AV307 syp-1(me17) V/nT1 [unc-?(n754) let-? qIs50] (IV;V). Balanced heterozygotes are GFP+ Unc and segregate GFP+ Unc (heterozygotes), non-GFP non-Unc syp-1(me17) homozygotes, and dead eggs (nT1 homozygotes). syp-1(me17) homozygotes produce 95% dead embryos and 38% males. Cytologically they lack chiasmata in diakinesis-stage oocytes, exhibit persistent polarized nuclear organization during earlier meiotic prophase, lack synaptonemal complexes, and exhibit unstable pairing of homologous chromosomes. qIs50 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter (F22B7.9) driving GFP in the intestine.
AV308 him-14(it21)/mnC1 [dpy-10(e128) unc-52(e444)] II. Heterozygotes are wild-type and segregate wild-type heterozygotes, DpyUncs (mnC1 homozygotes), and him-14 homozygotes that produce >95% dead embryos and 45% males. Among these surviving progeny, cytologically they have 12 univalents in diakinesis-stage oocytes owing to a failure to form crossovers during meiosis.
AV311 dpy-18(e364) unc-3(e151) meT7 (III;X;IV). Dpy. Unc. meT7 is an end-to-end-to-end fusion of chromosomes III, X, and V. The right end of III is fused to the left end of X, and the right end of X is fused to the left end of IV. Constructed by crossing eT5 and mnT12. meT7 homozygotes produce 92% viable progeny. meT7 heterozygotes are Him and produce many dead eggs.
AV322 swm-1(me87) him-5(e1490) V. Sperm activation in virgin males. Very poor sperm transfer.
AV630 meIs8 II. meIs8 [pie-1p::GFP::cosa-1 + unc-119(+)] II. Transgene contains a combination of cDNA and genomic sequences of cosa-1 including 212 bp of 3'UTR. GFP is expressed in the adult germline as 6 bright foci per nucleus (one per chromosome pair) from late pachytene through diplotene stages. Reference: Yokoo R, et al. Cell. 2012 Mar 30;149(1):75-87.
AX1295 gcy-35(ok769) I. Supresses aggregation and bordering phenotypes of npr-1(null) animals.
AX1296 gcy-36(db42) X. Supresses aggregation and bordering phenotypes of npr-1(null) animals.
AX1297 gcy-36(db66) X. Supresses aggregation and bordering phenotypes of npr-1(null) animals.
AX1305 gcy-34(ok1012) V; npr-1(ad609) X. Does not supress aggregation and bordering phenotypes of npr-1(null) animals.
AY1 nol-6(ac1) II. Temperature sensitive mutant. Grow at 15 to 20C. Sterile at 25C.
AY101 acIs101. acIs101 [F35E12.5p::GFP + rol-6(su1006)]. Rollers. Reference: (2010) J Bio Chem 285(14):10832-40.
AY102 pmk-1(km25) IV; acEx102. acEx102 [vha-6p::pmk-1::GFP + rol-6(su1006)]. Maintain by picking Rollers. Reference: (2010) J Bio Chem 285(14):10832-40.
AZ30 sma-1(ru18) V. Strong Sma phenotype. Null allele.  NOTE: Some animals Roll
AZ212 unc-119(ed3) ruIs32 III. ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Homozygous expression of GFP::H2B histone fusion in germline. pAZ132.
AZ217 unc-119(ed3) ruIs37 III. ruIs37 [myo-2p::GFP + unc-119(+)] III. Expresses GFP in the pharynx. pAZ119.
AZ218 unc-119(ed3) ruIs38 III. ruIs38 [partial myo-2 promoter::GFP + unc-119(+)]. Expresses GFP in the pharynx. pAZ119.
AZ235 unc-119(ed3) III; ruIs48. ruIs48 [pie-1::tubulin::GFP + unc-119(+)]. Homozygous expression of GFP::tubulin fusion in germline and early embryos. Insertion unmapped. pAZ147.