| BA901 |
spe-27(it110) dpy-20(e1282) IV. |
Temperature sensitive Dpy. Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps; males fertile. Spermatids arrest with spikes in pronase; form normal pseudopods in TEA. |
| BA925 |
spe-26(hc138) dpy-20(e1282) IV. |
Temperature-sensitive. Fertile and weak Dpy at 15C. Partially fertile at 20C. Sterile at 25C. Twitcher. Spermatogenesis arrests at the spermatocyte stage. |
| BA947 |
spe-27(hc161) IV. |
Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps; males fertile. |
| BA959 |
spe-29(it127) dpy-20(e1282) IV. |
Homozygous male/hermaphrodite line. Males are fertile. Hermaphrodites are sterile, but slightly leaky producing a few progeny (at 25C - ts not tested). In pronase, spermatids from males activate to form many long spikes, terminating at this stage. A very few (1-3 per worm) activate to form normal-looking, motile spermatozoons. |
| BA962 |
spe-29(it127) IV. |
Hermaphrodites are sterile; Males are fertile. Hermaphrodites lay oocytes (produce a few fertile eggs and many oocytes). Produce viable progeny when mated to males. Hermaphrodites produce 10X more self progeny at 20C (2.5/herm) than at either 16C or 25C. |
| BA963 |
spe-27(it132) IV. |
Temperature sensitive spe-27 allele. Hermaphrodites are sterile at 25C but produce 30-40 progeny/hermaphrodite at 16C. Males are fertile. |
| BA966 |
spe-27(it132) unc-22(e66) IV. |
Temperature sensitive spe-27 allele. Hermaphrodites sterile at 25C; hermaphrodites produce 30-40 progeny/hermaphrodite at 16C. Males cannot mate due to the unc-22 mutation. Maintain at 15C. |
| BA968 |
spe-29(it127) unc-24(e138) IV. |
Unc. Leaky sterile at 20C; tighter at 16C and 25C. Easier to propagate as if male-female strain. |
| BA969 |
spe-6(hc163) III; spe-27(it132) dpy-20(e1282) IV. |
Dpy. spe-6(hc163) is a recessive suppressor of spe-27(it132). spe-6(hc163) also suppresses spe-8, spe-12, spe-29 and other spe-27 alleles. Causes precocious spermatid activation. Fertile between 15-25C. |
| BA970 |
spe-6(hc163) III; spe-27(it132) unc-22(e66) IV. |
Twitcher Unc. spe-6(hc163) is a recessive suppressor of spe-27(it132). spe-6(hc163) also suppresses spe-8, spe-12, spe-29 and other spe-27 alleles. Causes precocious spermatid activation. Fertile between 15-25C. |
| BA971 |
spe-27(it132) dpy-20(e1282) IV. |
Temperature sensitive spe-27 allele. Leaky sterile at 20C. Males at 20C produce spermatids that form spikes in pronase. Males are fertile. Temperature sensitive dpy-20 allele. Maintain at 15C. |
| BA975 |
spe-6(hc163) III; spe-29(it127) dpy-20(e1282) IV. |
spe-6(hc163) suppresses the self-sterility of spe-29 in this strain. Self-sterile at 25C. |
| BA979 |
dpy-5(e61) spe-12(hc76) I; spe-6(hc163) III. |
spe-6(hc163) suppresses hermaphrodite self-sterility of hc76. Dpy. |
| BA984 |
spe-6(hc163) dpy-18(e364) III. |
Dpy. spe-6(hc163) suppresses self-sterility of spe-8, spe-12, spe-27, and spe-29 mutants. Causes precocious spermatide activation. Fertile between 15-25C. |
| BA989 |
spe-6(hc163) III; spe-27(it132) IV. |
spe-6(hc163) suppresses the ts self-sterile phenotype of spe-27(it132). Also suppresses spe-8, spe-12, and spe-29. Causes precocious spermatid activation. Lays eggs and oocytes. Males are weakly fertile. Fertile between 15-25C. |
| BA1013 |
spe-6(hc49) vab-7(e1562)/qC1 [dpy-19(e1259) glp-1(q339)] III; spe-27(it132) IV. |
Male/hermaphrodite line. Maintain at 15C to insure maintenance of male/hermaphrodite line. |
| BA1044 |
spe-27(it132) spe-29(it127) IV; him-5(e1490) V. |
Non-conditional hermaphrodite sterile. Males fertile. Self-progeny produced after mating. |
| BA1051 |
spe-12(hc76) I; spe-29(it127) IV; him-5(e1490) V. |
Self-sterile hermaphrodites; fertile males. Mated hermaphrodites produce self and outcross progeny. Self progeny are 33% males. |
| BA1061 |
dpy-18(e364) spe-6(hc49) ale-1(mc14)/qC1 [dpy-19(e1259) glp-1(q339)] III. |
Heterozygotes are WT and segregate WT, Sterile Dpys (Dpy is temperature sensitive), and dead eggs. ale-1 is a recessive embryonic lethal. |
| BA1069 |
F26F4.8(hc180) III. |
Deletion allele of F26F4.8. Homologous to Zn finger of Drosophila ovo transcription factor. Developmental defects in hind gut, germline and vulva. HES primers 106/107 outer and HES 108/109 inner. |
| BA1070 |
cdh-5(hc181) IV. |
Deletion allele of F08B4.2. Homologous to Drosophila fat cadherin. Primers HES 114/115 outer and HES 116/117 inner. |
| BA1073 |
dyf-5(hc183) I. |
Deletion allele of M04C9.5 (mak-kinase). No visible phenotype in hermaphrodites. Primers HES 186/187 outer and HES 188/189 inner. |
| BA1083 |
F43G6.6(hc184) II. |
Deletion allele of F43G6.6. No obvious phenotype in hermaphrodites. HES primers 162/163 outer and 164/165 inner. |
| BA1084 |
F09C12.2(hc185) II. |
Deletion allele of F09C12.2. No obvious phenotype in hermaphrodites. HES primers 122/123 outer and HES 124/125 inner. |
| BA1090 |
cav-2(hc191) V. |
No visible phenotype in hermaphrodites. Deletion allele of C56A3.7. |
| BA1091 |
C35D10.2(hc192) III. |
No visible phenotype in hermaphrodites. Primes HES 146/146 and HES 148/149. |
| BA1093 |
snf-10(hc194) V. |
Y32F6A.2 No visible phenotype in hermaphrodites. Primers: outer HES 22/228 and inner HES 229/230. |
| BB1 |
dcr-1(ok247)/unc-32(e189) III. |
Heterozygotes are WT and segregate WT heterozygote, Uncs (unc-32 homozygotes), and Steriles (dcr-1 homozygotes). |
| BB2 |
adr-1(gv6) I. |
Defective chemotaxis to volatile odorant. Low percentage (about 6%) have protruding vulva. |
| BB3 |
adr-2(gv42) III. |
Defective chemotaxis to volatile odorant. |
| BB4 |
adr-1(gv6) I; adr-2(gv42) III. |
Defective chemotaxis to volatile odorant. Low percentage (about 6%) have protruding vulva. |
| BB19 |
adr-1(tm668) I. |
Reduced lifespan, chemotaxis defective, co-suppression of transgenes in somatic cells. Maintain under normal conditions. Reference: Hundley HA, et al. RNA. 2008 Oct;14(10):2050-60. |
| BB21 |
adr-1(tm668) I; adr-2(ok735) III. |
Reduced lifespan, chemotaxis defective, co-suppression of transgenes in somatic cells. Maintain under normal conditions. Reference: Hundley HA, et al. RNA. 2008 Oct;14(10):2050-60. |
| BB22 |
adr-2(gv42) rde-4(ne299) III. |
RNAi deficient. Maintain under normal conditions. |
| BB23 |
adr-1(gv6) I; adr-2(gv42) III; rde-1(ne219) V. |
RNAi deficient. Maintain under normal conditions. Reference: Tonkin LA & BassBL. Science. 2003 Dec 5;302(5651):1725. Knight SW & Bass BL. Mol Cell. 2002 Oct;10(4):809-17. |
| BB24 |
adr-1(gv6) I; adr-2(gv42) rde-4(ne299) III. |
RNAi deficient. Maintain under normal conditions. Reference: Tonkin LA & BassBL. Science. 2003 Dec 5;302(5651):1725. |
| BB92 |
dcr-1(ok247) III; uuEx18. |
uuEx18 [dcr-1(wild-type) + dpy-30::mCherry]. Superficially wild-type. Reference: Welker N, et al. (2010) RNA 16:893-903. |
| BB93 |
dcr-1(ok247) III; uuEx19. |
uuEx19 [dcr-1(K39A) + dpy-30::mCherry]. Temperature-sensitive, sterile at 25C. Reference: Welker N, et al. (2010) RNA 16:893-903. |
| BB94 |
dcr-1(ok247) III; uuEx20. |
uuEx20 [dcr-1(D145N) + dpy-30::mCherry]. Temperature-sensitive, sterile at 25C. Reference: Welker N, et al. (2010) RNA 16:893-903. |
| BB95 |
dcr-1(ok247) III; uuEx21. |
uuEx21 [dcr-1(G492R) + dpy-30::mCherry]. Temperature-sensitive, sterile at 25C. Reference: Welker N, et al. (2010) RNA 16:893-903. |
| BC18 |
unc-22(s13) IV. |
Twitcher Unc. Heterozygotes twitch in 1% Nicotine. Recessive. |
| BC21 |
unc-22(s8) IV. |
Twitcher Unc. Recessive. Heterozygotes twitch in 1% Nicotine. |
| BC23 |
unc-22(s7) IV. |
Twitcher Unc. Recessive. Heterozygotes twitch in 1% Nicotine |
| BC96 |
unc-22(s16) IV. |
Twitcher Unc. Recessive. Heterozygotes twitch in 1% Nicotine. |
| BC107 |
bli-4(e937) dpy-14(e188) I. |
Blistered. Dpy (ts). |
| BC110 |
dpy-14(e188) unc-13(e51) let-85(s142)/unc-15(e73) I. |
Heterozygotes are WT and segregate WT, Unc and DpyUncLets. Lethal at L1. Maintain by picking WT. |
| BC112 |
dpy-14(e188) unc-13(e51) let-82(s85)/unc-15(e73) I. |
Heterozygotes are WT and segregate more WT, paralyzed Unc, and DpyUncLet (DpyUnc Larvae are abnormal). Maintain by picking WT. |
| BC115 |
dpy-14(e188) unc-13(e51) let-81(s88)/unc-15(e73) I. |
Heterozygotes are WT and segregate more WT, paralyzed Unc and DpyUncLethals. The DpyUncLets are abnormal larvae and die in early larval development. Pick WT to maintain. |
| BC119 |
blmp-1(s71) I. |
Dpy. |
| BC123 |
dpy-14(e188) unc-13(e51) let-84(s91)/unc-15(e73) I. |
Heterozygotes are WT and segregate more WT, paralyzed Unc and DpyUncLet. The DpyUncs are abnormal larvae that die in late larval development. Pick WT to maintain. |