Species Information: Caenorhabditis elegans

Name Caenorhabditis elegans
NCBI Taxonomy ID 6239

Caenorhabditis elegans strains available at the CGC

Strain Genotype Description
BA901 spe-27(it110) dpy-20(e1282) IV. Temperature sensitive Dpy. Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps; males fertile. Spermatids arrest with spikes in pronase; form normal pseudopods in TEA.
BA925 spe-26(hc138) dpy-20(e1282) IV. Temperature-sensitive. Fertile and weak Dpy at 15C. Partially fertile at 20C. Sterile at 25C. Twitcher. Spermatogenesis arrests at the spermatocyte stage.
BA947 spe-27(hc161) IV. Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps; males fertile.
BA959 spe-29(it127) dpy-20(e1282) IV. Homozygous male/hermaphrodite line. Males are fertile. Hermaphrodites are sterile, but slightly leaky producing a few progeny (at 25C - ts not tested). In pronase, spermatids from males activate to form many long spikes, terminating at this stage. A very few (1-3 per worm) activate to form normal-looking, motile spermatozoons.
BA962 spe-29(it127) IV. Hermaphrodites are sterile; Males are fertile. Hermaphrodites lay oocytes (produce a few fertile eggs and many oocytes). Produce viable progeny when mated to males. Hermaphrodites produce 10X more self progeny at 20C (2.5/herm) than at either 16C or 25C.
BA963 spe-27(it132) IV. Temperature sensitive spe-27 allele. Hermaphrodites are sterile at 25C but produce 30-40 progeny/hermaphrodite at 16C. Males are fertile.
BA966 spe-27(it132) unc-22(e66) IV. Temperature sensitive spe-27 allele. Hermaphrodites sterile at 25C; hermaphrodites produce 30-40 progeny/hermaphrodite at 16C. Males cannot mate due to the unc-22 mutation. Maintain at 15C.
BA968 spe-29(it127) unc-24(e138) IV. Unc. Leaky sterile at 20C; tighter at 16C and 25C. Easier to propagate as if male-female strain.
BA969 spe-6(hc163) III; spe-27(it132) dpy-20(e1282) IV. Dpy. spe-6(hc163) is a recessive suppressor of spe-27(it132). spe-6(hc163) also suppresses spe-8, spe-12, spe-29 and other spe-27 alleles. Causes precocious spermatid activation. Fertile between 15-25C.
BA970 spe-6(hc163) III; spe-27(it132) unc-22(e66) IV. Twitcher Unc. spe-6(hc163) is a recessive suppressor of spe-27(it132). spe-6(hc163) also suppresses spe-8, spe-12, spe-29 and other spe-27 alleles. Causes precocious spermatid activation. Fertile between 15-25C.
BA971 spe-27(it132) dpy-20(e1282) IV. Temperature sensitive spe-27 allele. Leaky sterile at 20C. Males at 20C produce spermatids that form spikes in pronase. Males are fertile. Temperature sensitive dpy-20 allele. Maintain at 15C.
BA975 spe-6(hc163) III; spe-29(it127) dpy-20(e1282) IV. spe-6(hc163) suppresses the self-sterility of spe-29 in this strain. Self-sterile at 25C.
BA979 dpy-5(e61) spe-12(hc76) I; spe-6(hc163) III. spe-6(hc163) suppresses hermaphrodite self-sterility of hc76. Dpy.
BA984 spe-6(hc163) dpy-18(e364) III. Dpy. spe-6(hc163) suppresses self-sterility of spe-8, spe-12, spe-27, and spe-29 mutants. Causes precocious spermatide activation. Fertile between 15-25C.
BA989 spe-6(hc163) III; spe-27(it132) IV. spe-6(hc163) suppresses the ts self-sterile phenotype of spe-27(it132). Also suppresses spe-8, spe-12, and spe-29. Causes precocious spermatid activation. Lays eggs and oocytes. Males are weakly fertile. Fertile between 15-25C.
BA1013 spe-6(hc49) vab-7(e1562)/qC1 [dpy-19(e1259) glp-1(q339)] III; spe-27(it132) IV. Male/hermaphrodite line. Maintain at 15C to insure maintenance of male/hermaphrodite line.
BA1044 spe-27(it132) spe-29(it127) IV; him-5(e1490) V. Non-conditional hermaphrodite sterile. Males fertile. Self-progeny produced after mating.
BA1051 spe-12(hc76) I; spe-29(it127) IV; him-5(e1490) V. Self-sterile hermaphrodites; fertile males. Mated hermaphrodites produce self and outcross progeny. Self progeny are 33% males.
BA1061 dpy-18(e364) spe-6(hc49) ale-1(mc14)/qC1 [dpy-19(e1259) glp-1(q339)] III. Heterozygotes are WT and segregate WT, Sterile Dpys (Dpy is temperature sensitive), and dead eggs. ale-1 is a recessive embryonic lethal.
BA1069 F26F4.8(hc180) III. Deletion allele of F26F4.8. Homologous to Zn finger of Drosophila ovo transcription factor. Developmental defects in hind gut, germline and vulva. HES primers 106/107 outer and HES 108/109 inner.
BA1070 cdh-5(hc181) IV. Deletion allele of F08B4.2. Homologous to Drosophila fat cadherin. Primers HES 114/115 outer and HES 116/117 inner.
BA1073 dyf-5(hc183) I. Deletion allele of M04C9.5 (mak-kinase). No visible phenotype in hermaphrodites. Primers HES 186/187 outer and HES 188/189 inner.
BA1083 F43G6.6(hc184) II. Deletion allele of F43G6.6. No obvious phenotype in hermaphrodites. HES primers 162/163 outer and 164/165 inner.
BA1084 F09C12.2(hc185) II. Deletion allele of F09C12.2. No obvious phenotype in hermaphrodites. HES primers 122/123 outer and HES 124/125 inner.
BA1090 cav-2(hc191) V. No visible phenotype in hermaphrodites. Deletion allele of C56A3.7.
BA1091 C35D10.2(hc192) III. No visible phenotype in hermaphrodites. Primes HES 146/146 and HES 148/149.
BA1093 snf-10(hc194) V. Y32F6A.2 No visible phenotype in hermaphrodites. Primers: outer HES 22/228 and inner HES 229/230.
BB1 dcr-1(ok247)/unc-32(e189) III. Heterozygotes are WT and segregate WT heterozygote, Uncs (unc-32 homozygotes), and Steriles (dcr-1 homozygotes).
BB2 adr-1(gv6) I. Defective chemotaxis to volatile odorant. Low percentage (about 6%) have protruding vulva.
BB3 adr-2(gv42) III. Defective chemotaxis to volatile odorant.
BB4 adr-1(gv6) I; adr-2(gv42) III. Defective chemotaxis to volatile odorant. Low percentage (about 6%) have protruding vulva.
BB19 adr-1(tm668) I. Reduced lifespan, chemotaxis defective, co-suppression of transgenes in somatic cells. Maintain under normal conditions. Reference: Hundley HA, et al. RNA. 2008 Oct;14(10):2050-60.
BB21 adr-1(tm668) I; adr-2(ok735) III. Reduced lifespan, chemotaxis defective, co-suppression of transgenes in somatic cells. Maintain under normal conditions. Reference: Hundley HA, et al. RNA. 2008 Oct;14(10):2050-60.
BB22 adr-2(gv42) rde-4(ne299) III. RNAi deficient. Maintain under normal conditions.
BB23 adr-1(gv6) I; adr-2(gv42) III; rde-1(ne219) V. RNAi deficient. Maintain under normal conditions. Reference: Tonkin LA & BassBL. Science. 2003 Dec 5;302(5651):1725. Knight SW & Bass BL. Mol Cell. 2002 Oct;10(4):809-17.
BB24 adr-1(gv6) I; adr-2(gv42) rde-4(ne299) III. RNAi deficient. Maintain under normal conditions. Reference: Tonkin LA & BassBL. Science. 2003 Dec 5;302(5651):1725.
BB92 dcr-1(ok247) III; uuEx18. uuEx18 [dcr-1(wild-type) + dpy-30::mCherry]. Superficially wild-type. Reference: Welker N, et al. (2010) RNA 16:893-903.
BB93 dcr-1(ok247) III; uuEx19. uuEx19 [dcr-1(K39A) + dpy-30::mCherry]. Temperature-sensitive, sterile at 25C. Reference: Welker N, et al. (2010) RNA 16:893-903.
BB94 dcr-1(ok247) III; uuEx20. uuEx20 [dcr-1(D145N) + dpy-30::mCherry]. Temperature-sensitive, sterile at 25C. Reference: Welker N, et al. (2010) RNA 16:893-903.
BB95 dcr-1(ok247) III; uuEx21. uuEx21 [dcr-1(G492R) + dpy-30::mCherry]. Temperature-sensitive, sterile at 25C. Reference: Welker N, et al. (2010) RNA 16:893-903.
BC18 unc-22(s13) IV. Twitcher Unc. Heterozygotes twitch in 1% Nicotine. Recessive.
BC21 unc-22(s8) IV. Twitcher Unc. Recessive. Heterozygotes twitch in 1% Nicotine.
BC23 unc-22(s7) IV. Twitcher Unc. Recessive. Heterozygotes twitch in 1% Nicotine
BC96 unc-22(s16) IV. Twitcher Unc. Recessive. Heterozygotes twitch in 1% Nicotine.
BC107 bli-4(e937) dpy-14(e188) I. Blistered. Dpy (ts).
BC110 dpy-14(e188) unc-13(e51) let-85(s142)/unc-15(e73) I. Heterozygotes are WT and segregate WT, Unc and DpyUncLets. Lethal at L1. Maintain by picking WT.
BC112 dpy-14(e188) unc-13(e51) let-82(s85)/unc-15(e73) I. Heterozygotes are WT and segregate more WT, paralyzed Unc, and DpyUncLet (DpyUnc Larvae are abnormal). Maintain by picking WT.
BC115 dpy-14(e188) unc-13(e51) let-81(s88)/unc-15(e73) I. Heterozygotes are WT and segregate more WT, paralyzed Unc and DpyUncLethals. The DpyUncLets are abnormal larvae and die in early larval development. Pick WT to maintain.
BC119 blmp-1(s71) I. Dpy.
BC123 dpy-14(e188) unc-13(e51) let-84(s91)/unc-15(e73) I. Heterozygotes are WT and segregate more WT, paralyzed Unc and DpyUncLet. The DpyUncs are abnormal larvae that die in late larval development. Pick WT to maintain.