Species Information: Caenorhabditis elegans

Name Caenorhabditis elegans
NCBI Taxonomy ID 6239

Caenorhabditis elegans strains available at the CGC

Strain Genotype Description
AA1 daf-12(rh257) X. daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele. Occasional abnormal dauers under exhausted conditions.
AA6 daf-12(rh84) X. daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele.
AA10 daf-12(rh286) X. Weak heterochronic phenotypes in seam, intestine, somatic gonad. Class V allele.
AA18 daf-12(rh61rh412) X. daf-d. Weak heterochronic phenotypes in seam, somatic gonad and intestine. Class III allele.
AA34 daf-12(rh61) X. daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele.
AA82 daf-12(rh284) X. Gonadal lead cell Mig. Weak heterochronic phenotype in intestine. Weakly daf-c at 25C. Class V allele.
AA83 daf-12(rh62rh157) X. daf-d. Strong heterochronic phenotypes in seam and intestine. Weak heterochronic phenotypes in somatic gonad. Class II allele.
AA85 daf-12(rh285) X. Strong heterochronic phenotypes in seam, somatic gonad, and intestine. Weakly daf-c at 15C. Class IV allele.
AA86 daf-12(rh61rh411) X. Daf-d, weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele.
AA87 daf-12(rh273) X. Daf-c, gonadal Mig, weak heterochronic phenotypes in intestine and seam. Class VI allele.
AA88 daf-12(rh193) X. Strong heterochronic phenotypes in seam, somatic gonad, and intestine. Heterochronic phenotypes less penetrant at 15C. Weakly daf-c at 25C. Class IV allele.
AA89 daf-12(rh274) X. daf-c. Gonadal Mig. Weak heterochronic phenotypes in intestine. Class VI allele.
AA107 nhr-48(ok178) X. ZK662.3 Homozygous. No obvious phenotype. Outer left primer sequence: TCTGAAGTTTGTGAGCCGTG. Outer right primer sequence: AGCGCCTAGATGAGCAACAT. Inner left primer sequence: TCCGTTGAATGCCATCTGTA. Inner right primer sequence: GGACGATGCACATGAGTTTG. Inner primer PCR product length: 3324 bp. Deletion size: 1956 bp.
AA120 dhIs26. dhIs26 [daf-12a::GFP + lin-15(+)]. DAF-12::GFP localized primarily in nucleus, except during mitosis. Expressed widely in most cells including tissues modified for dauer formation or by stage from embryo to adult, but most elevated and widespread during L2.
AA278 dhIs59. dhIs59 [Topo::daf-9::GFP + lin-15(+)]. Perinuclear expression in a ventral pair of bilateral neurons identified as the IL1Vs or URAVs in the anterior ganglia. By mid-L2, expression in the cytoplasm of the hypodermis, the syncitial epidermis, but absent from midline, epidermal seam cells. Levels peak around the L2 molt and diminish during L4. In some cases, transient expression seen in the L3 vulval blast cells. Also expressed within the hermaphrodite spermatheca starting in late L4 larvae and continuing eve in old adults. In males, expression in IL1V/URAVs and hypodermis but not somatic gonad. In dauer larvae, strong expression in IL1V/URAV and specifically extends into axonal but not dendritic processes. In post-dauer stages, expression in a pattern similar to reproductively growing animals, except expression is absent in the hypodermis. Grow at 20C. May still contain lin-15(n765) mutation in the background.
AA292 daf-36(k114) V. Mig on low cholesterol. Single daf-c at 27C, weak Mig. Strong expression in intestine at all stages. Grow at 20C.
AA408 din-1(dh127) II. daf-d, suppresses daf-12(rh61) daf-12(rh274) gonadal migration defects.
AA411 din-1(dh149) II. daf-d, suppresses daf-12(rh61) daf-12(rh274) gonadal migration defects.
AA426 dre-1(dh99) V. Precocious fusion of seam cells one stage earlier (prior to L3 molt); impenetrant gonadal migration defects; SynMig on daf-12 RNAi.
AA699 din-1(hd36) II. non-Daf. Temperature-sensitive phenotypes: at 20C half of the animals are egg-laying defective with occasional mispositioned gonadal arms; at 25C, 18% arrest as embryos: those animals that hatch usually display variable morphology defects in body and pharynx; nearly all animals that live to adults are small, clear, slightly uncoordinated, constipated, and virtually sterile. Maintain at 20C or below.
AA776 cyp-44A1(ok216) II.
AA790 lin-15B&lin-15A(n765) X; dhEx343. dhEx343 [din-1p::din-1E::GFP + lin-15(+)]. din-1s::GFP is detected in hypodermis, seam, intestine, and somatic gonad including the distal tip cells. din-1s is also expressed in neurons, vulval precursors, body wall muscle, pharynx, and all tissues with heterochronic phenotypes or remodeled during dauer. Expression is first detected in a few nuclei by the comma stage of embryogenesis. By hatching, din-1s was widely expressed, albeit weakly. Overall expression in most tissues is detected at various levels into adult and in dauer larvae. Animals with the array are GFP+ and non-Muv. Animals which have lost the array are Muv and non-GFP. din-1p::din-1E::GFP was produced by cloning into Fire Lab vector L3781.
AB1 C. elegans wild isolate. Reference WBG 10(2) 140-141 and WBG 8(2) 52. Caenorhabditis elegans wild isolate. (Tc1 pattern VII).
AB2 C. elegans wild isolate. Reference WBG 10(2) 140-141 and WBG 8(2) 52. Caenorhabditis elegans wild isolate. (Tc1 pattern VIII).
AB3 C. elegans wild isolate. Reference WBG 10(2) 140-141 and WBG 8(2) 52. Caenorhabditis elegans wild isolate. (Tc1 pattern VIII).
AB4 C. elegans wild isolate. Reference WBG 10(2) 140-141 and WBG 8(2) 52. Caenorhabditis elegans wild isolate. (Tc1 pattern VIII).
ABR1 pha-1(e2123) III; staEx1. staEx1 [T20F7.6p + pha-1(+)]. Empty vector control strain. Maintain at 25 degrees. Superficially wild-type. Reference: Greer EL et al Curr Biol 2007 Oct 9;17(19):1646-56.
ABR4 pha-1(e2123) III; staEx4. staEx4 [T20F7.6p(R81Q)::T20F7.6 + pha-1(+)]. Constitutively active T20f7.6 promoter construct (CA3). Maintain at 25 degrees. Superficially wild-type with increased lifespan and stress resistance. Reference: Greer EL et al Curr Biol 2007 Oct 9;17(19):1646-56.
ABR5 unc-119(ed3) III; staIs1. staIs1 [pie-1p::GFP + unc-119(+)]. Superficially wild-type. Maintain under normal conditions. Reference: This strain was used as the empty vector control in Greer EL et al Nature 2010 doi: 10.1038/nature09195.
ABR7 unc-119(ed3) III; staIs2. staIs2 [pie-1p::rbr-2::GFP + unc-119(+)]. Extended longevity. Maintain under normal conditions. Reference: This strain was used as LC Ppie-1::rbr-2::GFP (#3) in Greer EL et al Nature 2010 doi: 10.1038/nature09195.
ABR9 set-2(ok952) III; rbr-2(tm1231) IV. Reduced lifespan. Maintain under normal conditions. The parental rbr-2 strain was outcrossed 6x and the parental set-2 strain was outcrossed 2x. Reference: Greer EL et al Nature (2010) doi: 10.1038/nature09195.
AC68 unc-29(e1072) aph-2(zu181)/unc-13(e1091) lin-11(n566) I. Heterozygotes are WT and segregate WT, Unc Egls, and dead eggs.
AC257 ppk-3(n2668) X. Growth retardation, enlarged vacuoles (late endosomes and lysosomes) in intestine, epidermis, coelomocytes and pharynx. 27% embryonic lethality and 8% post-embryonic lethality.
AD186 egg-1(tm1071) III. Temperature sensitive sterile. Maintain at 20C. Fertility is <10% of WT at 25C.
AD189 unc-119(ed3) III; asIs2. asIs2 [pie-1p::GFP::egg-1 + unc-119(+)]. Oocyte membranes are GFP+.
AD200 unc-119(ed3) III; asIs1. asIs1 [pie-1p::GFP::egg-3 + unc-119(+)].
AD226 egg-3(tm1191)/mIn1 [mIs14 dpy-10(e128)] II. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP tm1191 homozygotes. Pick WT dim GFP and check for correct segregation of progeny to maintain.
AD238 asIs2. asIs2 [pie-1p::mCherry::egg-3].
AD265 nnIs2. nnIs2 [pie-1p::GFP::chs-1 + unc-119(+)].
AD266 egg-4(tm1508) egg-5(ok1781) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP tm1508 ok1781 homozygotes (maternal sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Parry JM, et al 2009 Current Biology 19(20):1752-7.
AD271 spe-38(eb44) I; him-5(e1490) V; asEx78. asEx78 [spe-38p::spe-38(cDNA)::spe-38 3'UTR + myo-3p::GFP]. Pick GFP+ to maintain. GFP+ worms are fertile; animals that have lost the array are sterile.
AE501 nhr-8(ok186) IV. Approx. 1.3 kb deletion - does not remove entire coding region, might not be null allele. No overt morphological or behavioral abnormalities. Homozygotes are 2-3X more sensitive to colchicine and chloroquine than are N2 animals.
AF1 +/szT1 [lon-2(e678)] I; dpy-8(e1321) unc-3(e151)/szT1 X. Heterozygotes are WT and segregate WT, DpyUnc, dead eggs and Lon males. Maintain by picking WT.
AG50 daf-7(e1372) dpy-1(e1) III. Maintain at 15C. Temperature-sensitive dauer constitutive: 100% dauers at 25C, leaky at 20C. Dpy. Crowds.
AG150 mat-2(ar104)/unc-4(e120) bli-1(e769) II. Heterozygotes are WT and segregate WT, UncBli, and Steriles which have an everted vulva. ar104 previously called evl-22.
AG151 cdc-25.1(nr2036)/dpy-5(e61) unc-13(e450) I. Heterozygotes are WT and segregate WT, DpyUncs and adult steriles. Original deletion from Axys Pharmaceuticals.
AG152 unc-85(e1414) bli-2(e768) dpy-10(e128) II. Unc and Dpy. Not Blistered: dpy-10 suppresses the appearance of the blisters.
AG165 san-1(av31) unc-13(e450) I. Unc. Reduced brood size. Larval lethal, larval arrest. Steriles. Bursts at vulva. Can be maintained as a homozygote.
AG166 mdf-2(av16) unc-17(e245) IV. Reduced brood size. Reduced hatching. Slow growth. Larval lethal. Larval arrest. Bursts at vulva. Suppresses the mat-3 one-cell arrest at 25C.
AG168 fzy-1(av15) unc-4(e120) II. Unc. Gain-of-function allele of fzy-1.