Laboratory Information

NameOD View on WormBase
Allele designationlt
HeadKaren Faye Oegema
InstitutionLudwig Institute for Cancer Research
Address 9500 Gilman Drive
CMM East, Room 3071
La Jolla 92093
United States
Website http://oegemadesailab.org/
Gene classes ani  cpar  czw  dgtr  hyls  kbp  mis  mvb  noca  perm  rod  ska  spdl  trcs 
tsg  yop  zwl  cdgs  cmt  cnep  nepr  tbca  tbcb  tbcc  tbcd  tbce 

Strains contributed by this laboratory

Strain Genotype Species Description
OD1 unc-119(ed3) III; ltIs1. C. elegans ltIs1[pIC22; pie-1 promoter::knl-3::GFP + unc-119(+)].
OD10 unc-119(ed3) III; ltIs6. C. elegans ltIs6 [pIC35; pie-1p::kbp-5::GFP-TEV-STag + unc-119(+)].
OD11 unc-119(ed3) III; ltIs7. C. elegans ltIs7 [(pIC41) pie-1p::kbp-4::GFP::TEV-STag + unc-119(+)].
OD13 unc-119(ed3) III; ltIs9. C. elegans ltIs9 [pie-1p::kbp-3::GFP::TEV-S Tag + unc-119(+)].
OD139 unc-119(ed3) III; ltIs37 IV; qaIs3502. C. elegans ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. qaIs3502[pie-1::YFP::LMN-1 + unc-119(+)].
OD141 unc-119(ed3) III; ltIs37 IV; qaIs3546. C. elegans ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. qaIs3546 [pie-1p::GFP::npp-8 + unc-119(+)]; npp-8 is CeNup155.
OD142 unc-119(ed3) III; ltIs78. C. elegans ltIs78 [(pKO5) pie-1::GFP::TEV::Stag::air-1 spliced coding + unc-119(+)].
OD176 unc-119(ed3) III; ltIs103. C. elegans ltIs103 [(pAA212) pie-1p::GFPLAP::cav-1 + unc-119(+)].
OD177 unc-119(ed3) III; ltIs104. C. elegans ltIs104 [(pAA277) pie-1p::GFP::LAP::vps-37 + unc-119(+)].
OD178 unc-119(ed3) III; ltIs105. C. elegans ltIs105 [(pAA280) pie-1p::GFP::LAP::MVB-12 + unc-119(+)].
OD179 unc-119(ed3) III; ltIs79; pwIs116. C. elegans ltIs79 [(pAA196) pie-1p::mCherry::rab-5 + unc-119(+)]. pwIs116 [rme-2p::rme-2::GFP::rme-2 3'UTR + unc-119(+)]. Maintain at 20-25C to reduce silencing of the array.
OD2442 ltSi794 II; unc-119(ed3) III. C. elegans ltSi794 [dpy-7p::vhhGFP4::zif-1::unc-54 3'UTR + Cbr-unc-119(+)] II. This transgenic strain mediates hypodermis-specific degradation of GFP-tagged proteins; can be combined with endogenous locus GFP-tagging or rescue of a null mutant with a GFP fusion to examine hypodermis-specific functions of target genes. Reference: Wang S, et al. Elife. 2015 Sep 15;4:e08649. Wang S, et al. http://biorxiv.org/content/early/2017/01/30/104398
OD27 unc-119(ed3) III; ltIs14. C. elegans ltIs14 [(pASM05) pie-1p::GFP-TEV-STag::air-2 + unc-119(+)].
OD2768 ltSi910 II; unc-119(ed3) III. C. elegans ltSi910 [elt-2p::vhhGFP4::zif-1::operon-linker::mCherry::his-11::tbb-2 3'UTR + Cbr-unc-119(+)] II. This transgenic strain mediates intestine-specific degradation of GFP-tagged proteins; can be combined with endogenous locus GFP-tagging or rescue of a null mutant with a GFP fusion to examine intestine-specific functions of target genes. Reference: Wang S, et al.  Development. 2017 Jun 15. pii: dev.150094. doi: 10.1242/dev.150094.
OD2769 ltSi911 II; unc-119(ed3) III. C. elegans Reference: Wang S, et al.  Development. 2017 Jun 15. pii: dev.150094. doi: 10.1242/dev.150094.
OD2770 ltSi912 II; unc-119(ed3) III. C. elegans ltSi912 [myo-3p::vhhGFP4::zif-1::operon-linker::mCherry::his-11::tbb-2 3'UTR + Cbr-unc-119(+)] II. This transgenic strain mediates body wall muscle-specific degradation of GFP-tagged proteins; can be combined with endogenous locus GFP-tagging or rescue of a null mutant with a GFP fusion to examine body wall muscle-specific functions of target genes. Reference: Wang S, et al.  Development. 2017 Jun 15. pii: dev.150094. doi: 10.1242/dev.150094.
OD2771 ltSi913 II; unc-119(ed3) III. C. elegans Control strain for OD2770
OD2772 ltSi914 II; unc-119(ed3) III. C. elegans ltSi914 [osm-6p::vhhGFP4::zif-1::operon-linker::mCherry::his-11::tbb-2 3'UTR + Cbr-unc-119(+)] II. This transgenic strain mediates sensory neuron-specific degradation of GFP-tagged proteins; can be combined with endogenous locus GFP-tagging or rescue of a null mutant with a GFP fusion to examine sensory neuron-specific functions of target genes. Reference: Wang S, et al.  Development. 2017 Jun 15. pii: dev.150094. doi: 10.1242/dev.150094.
OD2773 ltSi915 II; unc-119(ed3) III. C. elegans ltSi915 [osm-6p::zif-1::operon-linker::mCherry::histone::tbb-2 3'UTR + Cbr-unc-119(+)] II.  Control strain for OD2772, which mediates ciliated sensory neuron-specific degradation of GFP-tagged proteins.  Superficially wild type.  Reference: Wang S, et al.  Development. 2017 Jun 15. pii: dev.150094. doi: 10.1242/dev.150094.
OD2984 ltSi953 II; unc-119(ed3) III. C. elegans ltSi953 [mec-18p::vhhGFP4::zif-1::operon-linker::mKate::tbb-2 3'UTR + Cbr-unc-119(+)] II. This transgenic strain mediates touch neuron-specific degradation of GFP-tagged proteins; can be combined with endogenous locus GFP-tagging or rescue of a null mutant with a GFP fusion to examine touch neuron-specific functions of target genes. Reference: Wang S, et al. http://biorxiv.org/content/early/2017/01/30/104398
OD3 unc-119(ed3) III; ltIs24. C. elegans ltIs24 [(pAZ132) pie-1p::GFP::tba-2 + unc-119(+)].
OD31 unc-119(ed3) III; ltIs22. C. elegans ltIs22[pPM3; pie-1::GFP-TEV-STag::KNL-2 + unc-119(+)].
OD4027 ltSi448 II; unc-119(ed3) III; ltIs37 csr-1(tm892) IV. C. elegans ltSi448 [csr-1p::GFP::csr-1(re-encoded; GFP inserted after aa #5 of isoform b) + Cbr-unc-119(+)] II. ItIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. The csr-1 coding sequence in the transgene was re-encoded for RNAi-resistance (see Gerson-Gurwitz A, et al. Cell. 2016, Supplementary Figure S3A). [NOTE: it is not known if unc-119(ed3) is still present in the background of this strain.] Derived from strain OD1764; transgene rescues csr-1, so the balancer is not needed to maintain transgenic strain. Reference: Gerson-Gurwitz A, et al. Cell. 2016 Apr 7;165(2):396-409.
OD4028 ltSi240 II; unc-119(ed3) III; csr-1(tm892) IV. C. elegans ltSi240 [csr-1p::csr-1(re-encoded) + Cbr-unc-119(+)] II. The csr-1 coding sequence in the transgene was re-encoded for RNAi-resistance (see Gerson-Gurwitz A, et al. Cell. 2016, Supplementary Figure S3A). [NOTE: it is not known if unc-119(ed3) is still present in the background of this strain.] Derived from strain OD1463; transgene rescues csr-1, so the balancer is not needed to maintain transgenic strain. Reference: Gerson-Gurwitz A, et al. Cell. 2016 Apr 7;165(2):396-409.
OD56 unc-119(ed3) III; ltIs37 IV. C. elegans ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. his-58 genomic sequence is inserted at Spe I site. Robust expression of transgene in early embryos and germ line; some expression in somatic cells also detectable. Maintain under normal conditions. Reference: McNally et al., JCB (2006).
OD58 unc-119(ed3) III; ltIs38. C. elegans ltIs38 [pie-1p::GFP::PH(PLC1delta1) + unc-119(+)].
OD61 unc-119(ed3) III; ltIs41. C. elegans ltIs41[pAA5; pie-1::GFP-TEV-STag::CAR-1; unc-119(+)].
OD62 unc-119(ed3) III; ltIs42. C. elegans ltIs42[pAA19; pie-1::GFP-TEV-Stag::CAR-1deltaN; unc-119(+)].
OD63 unc-119(ed3) III; ltIs43/+. C. elegans ltIs43[pAA26; pie-1::GFP-TEV-STag::ZEN-4; unc-119(+)]. Insertion only viable when heterozygous. Pick non-Unc worms to maintain.
OD7 unc-119(ed3) III; ltIs3. C. elegans ltIs3[pIC31; pie-1 promoter::hcp-1::GFP-TEV-STag + unc-119(+)].
OD73 unc-119(ed3) III; ltIs38; ltIs24. C. elegans ltIs38 [pie-1p::GFP::PH(PLC1delta1) + unc-119(+)]. ltIs24 [pAZ132; pie-1::GFP::tba-2 + unc-119(+)].
OD76 unc-119(ed3) III; ltIs75. C. elegans ltIs75 [(pSK5) pie-1::GFP::TEV-STag::LacI + unc-119(+)].
OD8 unc-119(ed3) III; ltIs4. C. elegans ltIs4 [(pIC32) pie-1p::mis-12::GFP::TEV-STag + unc-119(+)].
OD83 unc-119(ed3) III; ltIs37 IV; qaIs3507. C. elegans ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. qaIs3507 [pie-1::GFP::lem-2 + unc-119(+)].
OD9 unc-119(ed3) III; ltIs5. C. elegans ltIs5 [(pIC36) pie-1p::kbp-1::GFP::TEV-STag + unc-119(+)].
OD95 unc-119(ed3) III; ltIs37 IV; ltIs38. C. elegans ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. ltIs38 [pie-1p::GFP::PH(PLC1delta1) + unc-119(+)]. Superficially wild-type. Maintain under normal conditions. Reference: McNally et al., JCB (2006).
TH27 unc-119(ed3) III; ddIs6 V. C. elegans ddIs6 [tbg-1::GFP + unc-119(+)] V.
TH32 unc-119(ed3) ruIs32 III; ddIs6 V. C. elegans ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. ddIs6 [tbg-1::GFP + unc-119(+)] V.
This laboratory hasn't submitted any alleles to the CGC.