Strain Information
| Name | OD2359 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | fzy-1(lt20::loxP)/mIn1[mIs14 dpy-10(e128)] II. |
| Description | CRISPR/Cas9 engineered deletion of fzy-1 in which the fzy-1 coding sequence was replaced by LoxP. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are wild-type with relatively dim pharyngeal GFP signal, and segregate wild-type dim GFP (heterozygotes), Dpy bright GFP (mIn1 homozygotes), and non-GFP fzy-1 homozygotes (larval arrest). Pick wild-type with dim GFP and check for correct segregation of progeny to maintain. gRNA sequence: Ggacgcacgcccggtagtgc Reference: Kim T, et al. Genes Dev. 2017 Jun 1;31(11):1089-1094. doi: 10.1101/gad.302067.117. PMID: 28698300. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x6 |
| Made by | Pablo Lara-Gonzalez |
| Laboratory | OD |
| Reference | PMID: 28698300 |
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