Strain Information
Name | OD3737 View On Wormbase |
---|---|
Species | C. elegans |
Genotype | cyb-3(lt110) V/nT1 [qIs51] (IV;V). |
Description | CRISPR/Cas9 engineered deletion of cyb-3. Heterozygotes are wild-type GFP+ and segregate mdf-2 null homozygotes (embryonic lethal), wild-type GFP+ heterozygotes, and arrested nT1[qIs51] aneuploids. Pick wild-type GFP+ and check for correct segregation of progeny to maintain. gRNA sequences: tcaggtcgacattcttggcc & gttatgggtatgagagcatt Reference: Lara-Gonzalez P, et al. Dev Cell. 2019 Nov 4;51(3):313-325.e10. doi: 10.1016/j.devcel.2019.09.005. PMID: 31588029. |
Mutagen | Crispr/Cas9 |
Outcrossed | x0 |
Made by | Pablo Lara-Gonzalez |
Laboratory | OD |
Reference | PMID: 31588029 |
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