Strain Information

Name OD3737   View On Wormbase
Species C. elegans
Genotypecyb-3(lt110) V/nT1 [qIs51] (IV;V).
DescriptionCRISPR/Cas9 engineered deletion of cyb-3. Heterozygotes are wild-type GFP+ and segregate mdf-2 null homozygotes (embryonic lethal), wild-type GFP+ heterozygotes, and arrested nT1[qIs51] aneuploids. Pick wild-type GFP+ and check for correct segregation of progeny to maintain. gRNA sequences: tcaggtcgacattcttggcc & gttatgggtatgagagcatt Reference: Lara-Gonzalez P, et al. Dev Cell. 2019 Nov 4;51(3):313-325.e10. doi: 10.1016/j.devcel.2019.09.005. PMID: 31588029.
MutagenCrispr/Cas9
Outcrossedx0
Made byPablo Lara-Gonzalez
Laboratory OD
Reference PMID: 31588029
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