Laboratory Information
| Name | GS View on WormBase |
|---|---|
| Allele designation | ar |
| Head | Iva Greenwald |
| Institution | Columbia University, New York, NY |
| Address | Columbia University Schapiro Building Room 212 530 West 120th Street New York 10027 United States |
| Website | http://cpmcnet.columbia.edu/dept/greenwaldlab/ |
| Gene classes | alx dsl evl hop lgx lrp lrx lst sel sum wwp ccar |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| GS10037 | arSi159 [rps-27p::GFP(flexon)::H2B::unc-54 3'UTR + Cbr-unc-119(+)] II; unc-119(ed3) III. | C. elegans | arSi159 [rps-27p::GFP(LoxP-flexon-LoxP)::H2B::unc-54 3'UTR + Cbr-unc-119(+)] II. Inserted into ttTi5605 (II). The first intron of GFP was replaced with a Flexon containing two loxP sites. High level GFP::H2B expression requires Cre expression from a second transgene. Reference: Wittes J & Greenwald I. (2024). New Flexon-based reagents for tissue-specific Auxin-Inducible Degradation and for characterizing Cre and Flp drivers in C. elegans. microPublication Biology. 10.17912/micropub.biology.001315. |
| GS107 | unc-32(e189) lin-12(n676n930) III; dpy-11(e224) sel-9(ar22) V. | C. elegans | DpyUnc. At 25C ar22 recessively suppresses the Egl defect of n676n930. At 15C ar22 dominantly suppresses the 2 AC-Egl phenotype of n676n930. Suppresses the vulval lineage defects and proximal mitosis defects of lin-12. Do not distribute this strain; other labs should request it from the CGC. |
| GS1214 | sel-12(ar171) unc-1(e538) X. | C. elegans | Egl. Unc. Do not distribute this strain; other labs should request it from the CGC. |
| GS1369 | emb-5(hc61) III. | C. elegans | Temperature sensitive maternal effect lethal if shifted from 15C to 25C at the L4 and later stages; sterile if it gets past embryogenesis before being shifted. Maintain at 15C. MJ61 contains a lin-12 allele (ar170) which results in 2 anchor cells. GS1369 was derived by outcrossing MJ61 to remove ar170. Do not distribute this strain; other labs should request it from the CGC. Added 10/2005: Zhuoyu Ni in Sylvia Lee's lab did not find the published missense mutation in this strain. It does have the ts sterile/lethal phenotype still. |
| GS1692 | unc-4(e120) II; arDp2 (II;f). | C. elegans | Pick non-Uncs to maintain. arDp2 is derived from mnC1, hence carries dpy-10(e128) and possibly unc-52(e444). arDp2 is a free duplication but does not pass at a high frequency. arDp2 is not stable as a balancer. It is prone to recombination; pick non-Unc and check for segregation of unc-4 and WT in progeny. Do not distribute this strain; other labs should request it from the CGC. |
| GS1829 | sel-2(n655) III. | C. elegans | Do not distribute this strain; other labs should request it from the CGC. |
| GS1894 | sel-12(ar131) X. | C. elegans | Egl. Do not distribute this strain; other labs should request it from the CGC. |
| GS1953 | sel-2(ar219) III. | C. elegans | Do not distribute this strain; other labs should request it from the CGC. |
| GS2381 | sel-5(ok149) III. | C. elegans | WT. Suppresses the egg-laying defect of lin-12(n302). Do not distribute this strain; other labs should request it from the CGC. |
| GS2447 | hop-1(ar179) I. | C. elegans | Do not distribute this strain; other labs should request it from the CGC. |
| GS2735 | spr-2(ar211) dpy-20(e1282) IV. | C. elegans | Dpy. Suppresses presenilin genes. Do not distribute this strain; other labs should request it directly from the CGC. |
| GS2744 | spr-2(ar199) dpy-20(e1282) IV. | C. elegans | Dpy. Suppresses presenilin genes. Do not distribute this strain; other labs should request it directly from the CGC. |
| GS3012 | spr-1(ar200) V. | C. elegans | ar200 does not display any apparent phenotype. It suppresses the Egl defect of both sel-12(ar171) and sel-12(ar131), and displays genetic interactions with various lin-12 and glp-1 alleles. Do not distribute this strain; other labs should request it from the CGC. |
| GS304 | evl-16(ar93)/unc-11(e47) dpy-5(e61) I. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. |
| GS305 | evl-17(ar94)/dpy-5(e61) unc-13(e51) I. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. |
| GS307 | dpy-5(e61) cye-1(ar95)/unc-13(e51) I. | C. elegans | Heterozygotes are WT and segregate WT, Uncs and Sterile Dpys which have an everted vulva. ar95 previously called evl-10(ar95). See also WBPaper00004382. Do not distribute this strain; other labs should request it from the CGC. |
| GS311 | unc-36(e251) evl-14(ar97)/dpy-17(e164) unc-32(e189) III. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. |
| GS314 | evl-3(ar100)/dpy-10(e128) unc-4(e120) II. | C. elegans | Heterozygotes are WT and segregate WT, Steriles with and Everted Vul and DpyUncs. Do not distribute this strain; other labs should request it from the CGC. |
| GS316 | unc-36(e251) evl-14(ar96)/dpy-17(e164) unc-32(e189) III. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. |
| GS3165 | spr-1(ar205) V. | C. elegans | ar205 does not display any apparent phenotype. It suppresses the Egl defect of both sel-12(ar171) and sel-12(ar131). Do not distribute this strain; other labs should request it from the CGC. |
| GS318 | evl-3(ar99)/dpy-10(e128) unc-4(e120) II. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. |
| GS320 | unc-36(e251) evl-8(ar102)/dpy-17(e164) unc-32(e189) III. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Unc Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. |
| GS326 | evl-20(ar103)/dpy-10(e128) unc-4(e120) II. | C. elegans | Heterozygotes are WT and segregate WT, Steriles with and Everted Vul and DpyUncs. See also WBPaper00005256. Do not distribute this strain; other labs should request it from the CGC. |
| GS327 | apc-1(ar104)/dpy-10(e128) unc-4(e120) II. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Segregates males, so may have a him- mutation? ar104 previously called evl-22 and mat-2. Do not distribute this strain; other labs should request it from the CGC. |
| GS328 | unc-36(e251) evl-19(ar98)/dpy-17(e164) unc-32(e189) III. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. |
| GS357 | unc-42(e270) arDf1 V/nT1 [unc-?(n754) let-?] (IV;V). | C. elegans | Heterozygotes are Unc and segregate Uncs and dead eggs. nT1 carries a dominant Unc mutation and a recessive lethal mutation. Do not distribute this strain; other labs should request it from the CGC. |
| GS3582 | unc-4(e120) II; arIs92. | C. elegans | arIs92[egl-17p::NLS-CFP-LacZ + unc-4(+) + ttx-3::GFP]. Do not distribute this strain; other labs should request it from the CGC. |
| GS3662 | dsl-1(ok810) IV. | C. elegans | WT phenotype. Do not distribute this strain; other labs should request it from the CGC. |
| GS367 | evl-23(ar106)/dpy-13(e184) unc-24(e138) IV. | C. elegans | Heterozygotes are WT and segregate WT, Steriles with and Everted Vul and DpyUncs. Do not distribute this strain; other labs should request it from the CGC. |
| GS368 | evl-13(ar107)/dpy-13(e184) unc-24(e138) IV. | C. elegans | Heterozygotes are WT and segregate WT, Steriles with and Everted Vul and DpyUncs. Do not distribute this strain; other labs should request it from the CGC. |
| GS369 | evl-7(ar108)/dpy-13(e184) unc-24(e138) IV. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. |
| GS370 | evl-12(ar109)/dpy-11(e224) unc-42(e270) V. | C. elegans | Heterozygotes are WT and segregate WT, Steriles with an Everted Vul and DpyUncs. Received new stock 10/2003. Do not distribute this strain; other labs should request it from the CGC. |
| GS3754 | sel-13(ok303) III; arIs51 IV; sel-7(n1253) unc-3(e151) X. | C. elegans | arIs51[cdh-3::GFP]. sel-13=T04A8.10. Reported strong daf-c; raise at 15 C (personal communication to the CGC). Do not distribute this strain; other labs should request it from the CGC. |
| GS3798 | arIs99. | C. elegans | arIs99 [dpy-7p::2Xnls::YFP]. Reference: Myers TR & Greenwald I, Dev Cell. 2005 Jan;8(1):117-23. Do not distribute this strain; other labs should request it from the CGC. |
| GS384 | fos-1(ar105)/dpy-11(e224) unc-42(e270) V. | C. elegans | Heterozygotes are WT and segregate WT, Steriles with an Everted Vul and DpyUncs. Do not distribute this strain; other labs should request it from the CGC. ar105 previously called evl-5(ar105). |
| GS387 | unc-36(e251) evl-14(ar112)/dpy-17(e164) unc-32(e189) III. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. |
| GS401 | let-418(ar113)/unc-46(e177) dpy-11(e224) V. | C. elegans | Heterozygotes are WT and segregate WT, Steriles with an Everted Vul and DpyUncs. ar113 pka evl-11(ar113). Do not distribute this strain; other labs should request it from the CGC. |
| GS402 | let-418(ar114)/unc-46(e177) dpy-11(e224) V. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. ar114 pka evl-11(ar114). Do not distribute this strain; other labs should request it from the CGC. |
| GS404 | evl-4(ar116)/dpy-10(e128) unc-4(e120) II. | C. elegans | Heterozygotes are WT and segregate WT, Steriles with an Everted Vul and DpyUncs. Do not distribute this strain; other labs should request it from the CGC. |
| GS408 | evl-2(ar119)/unc-85(e1414) dpy-10(e128) II. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted Vulva. CGC rec'd new stock from Iva Greenwald 5/2000. The unc-85 dpy-10 chromosome had been lost from this stock; for some reason it was difficult to maintain as a heterozygote. Do not distribute this strain; other labs should request it from the CGC. |
| GS416 | evl-6(ar120)/dpy-13(e184) unc-24(e138) IV. | C. elegans | Heterozygotes are WT (Dpyish) and segregate WT (Dpyish), DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. |
| GS419 | evl-3(ar118)/dpy-10(e128) unc-4(e120) II; him-5(e1467) V. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Throws males. Do not distribute this strain; other labs should request it from the CGC. |
| GS422 | evl-21(ar122) unc-36(e251)/unc-93(e1500) dpy-17(e164) III. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. |
| GS4249 | unc-4(e120) II; arIs82. | C. elegans | arIs82 [lin-12::GFP + egl-17p::lacZ + unc-4(+)]. Reference: Shaye DD, Greenwald I. Nature. 2002 Dec 12;420(6916):686-90. Do not distribute this strain; other labs should request it from the CGC. |
| GS431 | evl-24(ar124)/dpy-13(e184) unc-24(e138) IV. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. |
| GS433 | evl-4(ar101)/dpy-10(e128) unc-4(e120) II. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. |
| GS4335 | arIs41 II. | C. elegans | arIs41 [lin-12::GFP + rol-6(su1006)]. Rollers. Reference: Levitan D, Greenwald I. Development. 1998 Aug;125(16):3101-9. Do not distribute this strain; other labs should request it from the CGC. |
| GS454 | evl-9(ar121)/dpy-5(e61) unc-13(e51) I. | C. elegans | Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. |
| GS457 | evl-18(ar117) dpy-17(e164)/unc-36(e251) III. | C. elegans | Heterozygotes are WT and segregate WT, Uncs and Sterile Dpys which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. |
| GS460 | evl-15(ar126) dpy-20(e1282)/unc-26(e205) IV. | C. elegans | Heterozygotes are WT and segregate WT, Uncs and DpySteriles with an everted vulva. Recombines. Do not distribute this strain; other labs should request it from the CGC. |
| GS4815 | arIs98. | C. elegans | arIs98 [apx-1p::2xNLS::YFP + ceh-22p::GFP + dpy-20(+)]. References: Li J, Greenwald I. Curr Biol. 2010 Oct 26;20(20):1875-9. Karp X, Greenwald I. Proc Natl Acad Sci USA. 2013 Feb 5;110(6):2181-6. Do not distribute this strain; other labs should request it from the CGC. |
| GS4892 | arIs131 III. | C. elegans | arIs131 [lag-2p::2xNLS::YFP + ceh-22p::GFP + pha-1(+)] III. References: Li J, Greenwald I. Curr Biol. 2010 Oct 26;20(20):1875-9. Zhang X, Greenwald I. Genetics. 2011 Aug;188(4):847-58. Do not distribute this strain; other labs should request it from the CGC. |
| GS5231 | arIs116 X. | C. elegans | arIs116 [lst-5p::2xNLS::YFP + ttx-3p::GFP + pha-1(+)] X. References: Li J, Greenwald I. Curr Biol. 2010 Oct 26;20(20):1875-9. Karp X, Greenwald I. Proc Natl Acad Sci USA. 2013 Feb 5;110(6):2181-6. Do not distribute this strain; other labs should request it from the CGC. |
| GS60 | unc-32(e189) lin-12(n676n930) III. | C. elegans | Unc. Temperature sensitive hypomorphic lin-12 allele. At restrictive temperature of 25C: 100% Egl in unc-32 background (95% Egl without unc-32); 30% have 2 anchor cells, some proximal mitosis, abnormal vulval lineages but has opening; leaks some eggs; mates with males. At permissive temperature of 15C: 93% Egl(+) and 7% Egl(-) [lin-12(d)-like: no anchor cell and no vulval opening]. Do not distribute this strain; other labs should request it from the CGC. |
| GS6107 | arIs107. | C. elegans | arIs107 [mir-61p::2xNLS::YFP + ttx-3p::GFP + pha-1(+)]. References: Yoo AS, Greenwald I. Science. 2005 Nov 25;310(5752):1330-3. Do not distribute this strain; other labs should request it from the CGC. |
| GS776 | unc-32(e189) lin-12(n676n930) III; unc-42(e270) sel-11(ar39) V. | C. elegans | Unc. At 25C ar39 recessively suppresses the Egl phenotype of n676n930. At 15C a high percentage of hermaphrodites have a 0 AC-Egl phenotype. ar39 recessively suppresses vulval lineage defects and proximal mitosis. Do not distribute this strain; other labs should request it from the CGC. |
| GS807 | unc-32(e189) lin-12(n676n930) III; sqt-3(sc8) sel-1(e1948) V. | C. elegans | RollerUnc. At 25C e1948 recessively suppresses the Egl phenotype of n676n930. At 15C a high percentage of hermaphrodites have a 0 AC-Egl phenotype. e1948 recessively suppresses vulval lineage defects and proximal mitosis. sc8 previously called rol-4(sc8). See also WBPaper00002448. Do not distribute this strain; other labs should request it from the CGC. |
| GS8190 | arTi85. | C. elegans | arTi85 [lin-31p::ERK::KTR::mClover::T2A::mCherry::his-11::unc-54 3'UTR + rps-27p::NeoR::unc-54 3'UTR]. Superficially wild-type. arTi85 transgene is a single-copy transposon insertion expressing a fluorescent protein (ERK::KTR::mClover) that reports MPK-1 kinase activity in vulval precursor cells (VPCs). A nuclear histone marker is co-expressed (mCherry::H2B). Reference: de la Cova C, et al. Developmental Cell. 2017 Vol. 42(5):542-553. |
| GS8255 | arTi101. | C. elegans | arTi101 [lin-31p::ERK::KTR(S43A, T55A, S62A)::mClover::T2A::mCherry::his-11::unc-54 3'UTR + rps-27p::NeoR::unc-54 3'UTR]. Superficially wild-type. arTi101 transgene is a single-copy transposon insertion expressing a mutant, unphosphorylated form of a fluorescent protein (ERK::KTR::mClover) that reports MPK-1 kinase activity in vulval precursor cells (VPCs). A nuclear histone marker is co-expressed (mCherry::H2B). Use arTi101 as a negative control for transgene arTi85. Reference: de la Cova C, et al. Developmental Cell. 2017 Vol. 42(5):542-553. |
| GS8513 | arTi145 II. | C. elegans | arTi145 [ckb-3p::mCherry::his-58::unc-54 3'UTR] II. miniMos insertion with bright expression due to perdurance mediated by the histone; expressed in all cells from Z1/Z4 until somatic gonad blast cells divide, so labels the entire somatic gonad primordium in continuous L2 or dauer larvae. Reference: Attner et al., 2019, Current Biology 29, 1-7. |
| GS8729 | arSi12. | C. elegans | arSi12 [mex-5p::ERK::KTR::GFP(smu-1 introns)::T2A::mCherry::his-11::tbb-2 3'UTR]. arSi12 is a single-copy CRISPR/Cas9-engineered insertion expressing a fluorescent protein (ERK::KTR::GFP) that reports MPK-1 kinase activity in the germline. A nuclear histone marker is co-expressed (mCherry::H2B). Reference: de la Cova C, et al. Developmental Cell. 2017 Vol. 42(5):542-553. |
| GS8752 | arSi15. | C. elegans | arSi15 [mex-5p::ERK::KTR(S43A, T55A, S62A)::GFP(smu-1 introns)::T2A::mCherry::his-11::tbb-2 3'UTR]. arSi15 is a single-copy CRISPR/Cas9-engineered insertion expressing a mutant, unphosphorylated form of a fluorescent protein (ERK::KTR::GFP) that reports MPK-1 kinase activity in the germline. A nuclear histone marker is co-expressed (mCherry::H2B). Use arSi15 as a negative control for transgene arSi12. Reference: de la Cova C, et al. Developmental Cell. 2017 Vol. 42(5):542-553. |
| GS878 | unc-32(e189) lin-12(n676n930) III; lon-3(e2175) sel-10(ar41) V. | C. elegans | Long. Unc. At 15C ar41 suppresses the 2 AC (anchor cell) phenotype of n676n930. (Strain GS878 does not contain sel(arX) and therefore does not suppress the Egl phenotype at 25C.) See also WBPaper00002966. Do not distribute this strain; other labs should request it from the CGC. |
| GS883 | dpy-5(e61) sel(ar40) I; unc-32(e189) lin-12(n676n930) III. | C. elegans | DpyUnc. ar40 is a semi-dominant suppressor. At 25C ar40 suppresses the Egl phenotype of ne676n930. At 15C a high percentage of hermaphrodites have a 0 AC-Egl phenotype. ar40 suppresses proximal mitosis. ar40 does not suppress vulval lineage defects. Do not distribute this strain; other labs should request it from the CGC. |
| GS8949 | hlh-2(ar623[gfp::hlh-2]) I. | C. elegans | GFP tag inserted into endogenous hlh-2 locus produces a fully-functional GFP-tagged form of HLH-2. Reference: Attner et al., 2019, Current Biology 29, 1-7. |
| GS925 | let-54(s44) unc-22(s7) IV/nT1 [unc-?(n754) let-?] (IV;V). | C. elegans | Heterozygotes are Unc and segregate Unc, dead eggs and lethal Twitchers. Lethal early larval (L1-L2). Maintain by picking Unc. Do not distribute this strain; other labs should request it from the CGC. |
| GS9402 | arTi362 | C. elegans | arTi362 [rps-27p::TIR1(lox2272-flexon-lox2272)::unc-54 3'UTR + HygroR] V (-19.98). The first intron of TIR1 was replaced with a Flexon containing two lox2272 sites. TIR1 is expressed at a high level in lineages where Cre is expressed. Reference: Wittes J & Greenwald I. (2024). New Flexon-based reagents for tissue-specific Auxin-Inducible Degradation and for characterizing Cre and Flp drivers in C. elegans. microPublication Biology. 10.17912/micropub.biology.001315. |
| GS9407 | arTi361 [rps-27p::GFP(lox2272-flexon-lox2272)::H2B::unc-54 3'UTR + NeoR] I. | C. elegans | arTi361 [rps-27p::GFP(lox2272-flexon-lox2272)::H2B::unc-54 3'UTR + NeoR] I (-12.74). First intron of GFP is replaced with a Flexon containing two lox2272 sites. GFP::H2B is expressed at a high level in lineages where Cre is expressed with a second transgene. Transgene maps to -12.74 (I). Reference: Wittes J & Greenwald I. (2024). New Flexon-based reagents for tissue-specific Auxin-Inducible Degradation and for characterizing Cre and Flp drivers in C. elegans. microPublication Biology. |
| GS9801 | rde-1(ar660) V. | C. elegans | rde-1(ar660)[rde-1(lox2272-flexon-lox2272)]. 9th intron of rde-1 replaced with a flexon - an artificial exon flanked by artificial introns that each contain a lox2272 site (a flexon). Severely reduces rde-1 function, function can be restored upon excision of the flexon by Cre recombinase. Reference: Shaffer JM & Greenwald I. Proc Natl Acad Sci U S A. 2022 Jan 18;119(3):e2117451119. doi: 10.1073/pnas.2117451119. PMID: 35027456. |
| GS9820 | arTi443 | C. elegans | arTi443 [rps-27p::TIR1(F79G)(lox2272-flexon-lox2272)::unc-54 3'UTR + HygroR] V (+21.99). The first intron of TIR1(F79G) was replaced with a Flexon containing two lox2272 sites. TIR1(F79G) is expressed at a high level in lineages where Cre is expressed with a second transgene. Reference: Wittes J & Greenwald I. (2024). New Flexon-based reagents for tissue-specific Auxin-Inducible Degradation and for characterizing Cre and Flp drivers in C. elegans. microPublication Biology. 10.17912/micropub.biology.001315. |
| GS9847 | arTi452 [rps-27p::GFP(LoxP-flexon-LoxP)::H2B::unc-54 3'UTR + NeoR] I. | C. elegans | arTi452 [rps-27p::GFP(LoxP-flexon-LoxP)::H2B::unc-54 3'UTR + NeoR] I (-4.81). First intron of GFP is replaced with a Flexon containing two loxP sites. GFP::H2B is expressed at a high level in lineages where Cre is expressed with a second transgene. Reference: Wittes J & Greenwald I. (2024). New Flexon-based reagents for tissue-specific Auxin-Inducible Degradation and for characterizing Cre and Flp drivers in C. elegans. microPublication Biology. 10.17912/micropub.biology.001315. |
| GS9922 | arTi461 [rps-27p::GFP(FRT-flexon-FRT)::H2B::unc-54 3'UTR + NeoR] I. | C. elegans | arTi461 [rps-27p::GFP(FRT-flexon-FRT)::H2B::unc-54 3'UTR + NeoR] I (-3.80). The first intron of GFP was replaced with a Flexon containing two FRT sites. GFP::H2B is expressed at a high level in lineages where Flp recombiase is expressed with a second transgene. Flexon contains two FRT sites. Reference: Wittes J & Greenwald I. (2024). New Flexon-based reagents for tissue-specific Auxin-Inducible Degradation and for characterizing Cre and Flp drivers in C. elegans. microPublication Biology. 10.17912/micropub.biology.001315. |
| MT2056 | sup-10(n983) X. | C. elegans | Class 3 (neomorphic) allele. Uncoordinated, rubberband paralysis like unc-93(e1500). Suppressed by intragenic revertants and unc-93 alleles. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| ar104 | Allele | ||
| ar202 | Allele | substitution | |
| ar221 | Allele | ||
| ar102 | Allele | ||
| ar98 | Allele | ||
| ar228 | Allele | substitution | nonsense |
| ar232 | Allele | substitution | nonsense |
| ar229 | Allele | substitution | |
| ar226 | Allele | substitution | |
| ar22 | Allele | substitution | |
| ar171 | Allele | substitution | nonsense |
| ar131 | Allele | substitution | |
| ar62 | Allele | ||
| ar219 | Allele | substitution | nonsense |
| ar60 | Allele | ||
| ar179 | Allele | deletion | |
| ar465 | Allele | substitution | |
| ar466 | Allele | ||
| ar467 | Allele | ||
| ar472 | Allele | ||
| ar479 | Allele | substitution | nonsense |
| ar492 | Allele | substitution | |
| ar493 | Allele | substitution | |
| ar498 | Allele | ||
| ar506 | Allele | substitution | nonsense |
| ar211 | Allele | substitution | |
| ar199 | Allele | deletion | |
| ar200 | Allele | substitution | splice_site |
| ar93 | Allele | ||
| ar94 | Allele | ||
| ar95 | Allele | substitution | nonsense |
| ar97 | Allele | ||
| ar100 | Allele | ||
| ar96 | Allele | substitution | nonsense |
| ar205 | Allele | substitution | nonsense |
| ar99 | Allele | ||
| ar103 | Allele | substitution | nonsense |
| ar106 | Allele | ||
| ar107 | Allele | ||
| ar108 | Allele | ||
| ar109 | Allele | ||
| ar105 | Allele | substitution | nonsense |
| ar112 | Allele | substitution | splice_site |
| ar113 | Allele | substitution | nonsense |
| ar114 | Allele | substitution | nonsense |
| ar116 | Allele | ||
| ar119 | Allele | ||
| ar120 | Allele | ||
| ar118 | Allele | ||
| ar122 | Allele | ||
| ar124 | Allele | ||
| ar101 | Allele | ||
| ar90 | Allele | substitution | nonsense |
| ar121 | Allele | ||
| ar117 | Allele | ||
| ar126 | Allele | ||
| ar39 | Allele | substitution | |
| ar41 | Allele | substitution | nonsense |
| ar40 | Allele | ||
| ar170 | Allele | substitution |