Strain Information

Name GS8752   View On Wormbase
Species C. elegans
GenotypearSi15.
DescriptionarSi15 [mex-5p::ERK::KTR(S43A, T55A, S62A)::GFP(smu-1 introns)::T2A::mCherry::his-11::tbb-2 3'UTR]. arSi15 is a single-copy CRISPR/Cas9-engineered insertion expressing a mutant, unphosphorylated form of a fluorescent protein (ERK::KTR::GFP) that reports MPK-1 kinase activity in the germline. A nuclear histone marker is co-expressed (mCherry::H2B). Use arSi15 as a negative control for transgene arSi12. Reference: de la Cova C, et al. Developmental Cell. 2017 Vol. 42(5):542-553.
MutagenCRISPR/Cas9 homologous recombination
Outcrossedx0
Made byClaire de la Cova
Laboratory GS
Reference de la Cova, C., Townley, R., Regot, S., and Greenwald, I. A real-time biosensor for ERK activity reveals signaling dynamics during C. elegans cell fate specification. Developmental Cell. 2017 Vol. 42(5):542-553. doi: 10.1016/j.devcel.2017.07.014. PMID: 28826819.
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