Laboratory Information
| Name | DCR View on WormBase |
|---|---|
| Allele designation | ola |
| Head | Daniel Colon-Ramos |
| Institution | Yale University, New Haven, CT |
| Address | Yale School of Medicine BCMM 439 295 Congress Ave New Haven 06511 United States |
| Gene classes | pfk cima pfkb |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| BJH728 | unc-26(pek288[R216Q]) IV. | C. elegans | unc-26(pek288) is a CRISPR-engineered R216Q substitution in unc-26/synaptojanin 1 associated with early-onset Parkinsonism (EOP) (Quadri et al., 2013; Krebs et al., 2013). This allele shows abnormal focal accumulation of ATG-9 in presynaptic nerve terminals, defects in activity-induced synaptic autophagy, and defects in sustained neurotransmission and locomotory behaviors in aging animals. Reference: Yang S, et al. Neuron. 2022 Mar 2;110(5):824-840.e10. PMID: 35065714 |
| COP2412 | atg-9(ola511[delta AP]) V. | C. elegans | ola511 is aCRISPR-engineered allele deleting a conserved sorting motif in ATG-9, causing a 2- to 3-fold decrease in LGG-1-containing puncta (and therefore autophagosomes) in the AIY neurites. Reference: Yang S, et al. Neuron. 2022 Mar 2;110(5):824-840.e10. |
| CX18334 | cat-1 (ky1118 *ky1101) X. | C. elegans | Flippase-dependent cat-1 knockout. cat-1 coding sequence flanked by FRT sites, allowing cell-specific knockout of cat-1 in a Flippase-dependent manner. Upon recombination, the cat-1 coding sequence is removed and cytosolic mCherry marker is expressed. Part of SynaptoTagMe toolkit for examining cell-specific transmission. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
| DCR1017 | cima-1(wy84) IV; wyIs45 X; olaEx603. | C. elegans | wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx603 [rab-3p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx605 does not rescue AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR1023 | cima-1(wy84) IV; wyIs45 X; olaEx609. | C. elegans | wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx609 [aex-3p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx609 does not rescue AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR1078 | cima-1(wy84) IV; wyIs45 X; olaEx551. | C. elegans | wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx551 [cima-1p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx551 rescues AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR1099 | cima-1(wy84) IV; wyIs45 X, olaEx644. | C. elegans | wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx644 [ttx-3p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx642 does not rescue AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR1102 | cima-1(wy84) IV; wyIs45 X; olaEx647. | C. elegans | wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx647 [hlh-17p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx647 does not rescue AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR1288 | cima-1(wy84) IV; wyIs45 X; olaEx765. | C. elegans | wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx765 [dpy-7p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx765 rescues AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR1301 | cima-1(wy84) IV; wyIs45 X; olaEx775. | C. elegans | wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx775 [dpy-4p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx605 rescues AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR1337 | nsIs105 I; cima-1(wy84) IV; wyIs45 X; olaEx805. | C. elegans | nsIs105 [hlh-17p::GFP] I. wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx805 [hlh-17p::caspase12 + hlh-17p::caspase17 + ttx-3p::mCherry + glr-3p::mCherry + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. Ablation of CEPsh glia partially suppresses AIY presynaptic maintenance defects in cima-1(wy84) mutants. nsIs105 (hlh-17::GFP) expression labels CEPsh glia. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR1673 | olaEx987. | C. elegans | olaEx987 [ttx-3p::mCherry::rab-3 + hlh-17p::CD4::GFP1-10 + ttx-3p::CD4::GFP11 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx987 labels AIY presynaptic sites with mCherry, and AIY and CEPsh contact with GFP. oleEx987 contains GRASP (GFP Reconstitution Across Synaptic Partners) constructs using two GFP fragments, GFP1-10 and GFP11, that can reconstitute a functional GFP molecule only when they are in close proximity. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR1690 | cima-1(wy84) IV; egl-15(n484) wyIs45 X; olaEx1004. | C. elegans | wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx1004 [F25B3.3p::egl-15(5A) + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx1004 does not rescue egl-15(n484) suppression of cima-1 AIY presynaptic defects. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR1710 | cima-1(wy84) IV; egl-15(n484) wyIs45 X; olaEx1015. | C. elegans | wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx1015 [dpy-7p::egl-15(5A) + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx1015 rescues egl-15(n484) suppression of cima-1 AIY presynaptic defects. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR1779 | cima-1(wy84) IV; egl-15(n484) wyIs45 X; olaEx1054. | C. elegans | wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx1054 [dpy-7p::egl-15(5A)(ecto) + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx1054 rescues egl-15(n484) suppression of cima-1 AIY presynaptic defects. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR2188 | olaEx1316. | C. elegans | olaEx1316 [ttx-3p::CD4::GFP11 + glr-3p::CD4::GFP1-10 + ttx-3p::mCherry::rab-3 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx1316 labels AIY presynaptic sites with mCherry, and AIY and RIA contact with GFP. oleEx1316 contains GRASP (GFP Reconstitution Across Synaptic Partners) constructs using two GFP fragments, GFP1-10 and GFP11, that can reconstitute a functional GFP molecule only when they are in close proximity. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR2335 | cima-1(wy84) IV; wyIs45 X; olaEx1411. | C. elegans | wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx1411 [dpy-7p::egl-15(5A)::HA + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR3682 | inx-1(tm3524) X; olaEx2136. | C. elegans | olaEx2136 [inx-1p::inx-1::SL2::GFP + unc-122p::RFP]. Pick animals with RFP+ expression in coelomocytes to maintain. Rescues the isothermal tracking phenotype of inx-1 mutants. Reference: Almoril-Porras A, et al. Cell. 2025 Jan 9;188(1):89-103.e13. doi: 10.1016/j.cell.2024.11.037. PMID: 39742807. |
| DCR3791 | pfk-1.1(ola72) X. | C. elegans | Diffuse distribution of synaptic vesicle markers (SNB-1, CAT-1, and RAB-3) under hypoxic conditions. pfk-1.1(ola72) causes C562Y missense mutation. Reference: Jang et al. Neuron. 2016 Apr 20;90(2):278-91. |
| DCR4521 | atg-9(ola274[atg-9::gfp]) V. | C. elegans | This endogenous tag on ATG-9 was made from a CRISPR event that used a method described by Dickinson et al. 2015 to efficiently insert GFP in replace of the stop codon of atg-9. Reference: Stavoe AK, et al. Dev Cell. 2016 Jul 25;38(2):171-85. |
| DCR4750 | olaIs35 X. | C. elegans | olaIs35 [ttx-3Gp::eGFP::lgg-1 + ttx-3Gp::mCherry + unc-122p::RFP]. Integrated transgene allows visualization of autophagosomes in a single neuron (AIY). "ttx-3G" refers to genomic fragment containing AIY motif described in Bertrand & Hobert Dev Cell. 2009 Apr;16(4):563-75. References: Stavoe AK, et al. Dev Cell. 2016 Jul 25;38(2):171-85. Hill SE, et al. Dev Cell. 2019 Mar 8. pii: S1534-5807(19)30104-2. |
| DCR5037 | inx-1(ola278) X; olaEx2986. | C. elegans | olaEx2986 [ttx-3p::SL2::nCRE::unc-54 3'UTR + unc-122::RFP]. Pick animals with RFP+ expression in coelomocytes to maintain. Isothermal tracking phenotype during thermotaxis. inx-1(ola278) is an engineered floxed allele of inx-1 allowing conditional knockdown using Cre recombinase. Reference: Almoril-Porras A, et al. Cell. 2025 Jan 9;188(1):89-103.e13. doi: 10.1016/j.cell.2024.11.037. PMID: 39742807. |
| DCR5765 | atg-4.2(ola316) IV. | C. elegans | Dominant negative allele of autophagy protease atg-4.2. Animals appear slightly dumpy. In neurons, autophagic vacuoles accumulate abnormally in the soma. Reference: Hill SE, et al. Dev Cell. 2019 Mar 8. pii: S1534-5807(19)30104-2. |
| DCR744 | cima-1(wy84) IV; wyIs45 X. | C. elegans | wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. AIY presynaptic maintenance defect at adult stage (synapses appears in asynaptic Zone 3 region). Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR8881 | olaEx5329. | C elegans | olaEx5329 [rab-3p::HYlight + elt-7p::mCherry]. Pick mCherry+ animals to maintain. Pan-neuronal expression of HYlight, a codon-optimized biosensor that responds to changing levels of FBP in cells. Reference: Wolfe AD, et al. Proc Natl Acad Sci U S A. 2024 Jan 16;121(3):e2314699121. doi: 10.1073/pnas.2314699121. PMID: 38198527. |
| DCR8892 | olaEx5331. | C elegans | olaEx5331 [rab-3p::HYlight-RA + elt-7p::mCherry]. Pick mCherry+ animals to maintain. Pan-neuronal expression of HYlight-RA, a reduced affinity version of the FBP biosensor that does not respond to changes in concentration of the FBP metabolite during hypoxia in vivo. Can be used as a negative control for HYlight sensor in strain DCR8881. Reference: Wolfe AD, et al. Proc Natl Acad Sci U S A. 2024 Jan 16;121(3):e2314699121. doi: 10.1073/pnas.2314699121. PMID: 38198527. |
| DCR9089 | olaIs138 IV. | C elegans | olaIs138 [ttx-3p::SL2::HYlight::let-858 3'UTR + elt-7p::mCherry] IV. Expression of HYlight, a codon-optimized biosensor that responds to changing levels of FBP in cells, in the neuron pair AIY. Derived by UV/TMP insertion of the olaEx5367 transgene. Reference: Wolfe AD, et al. Proc Natl Acad Sci U S A. 2024 Jan 16;121(3):e2314699121. doi: 10.1073/pnas.2314699121. PMID: 38198527. |
| DCR9211 | unc-17(ola503[unc-17::GFP FLP-on]) IV. | C. elegans | GFP FLP-on inserted at C-terminus of endogenous unc-17 locus. Part of SynaptoTagMe toolkit for examining cell-specific cholinergic transmission. See https://elifesciences.org/reviewed-preprints/108675 for sequencing information. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
| DCR9265 | unc-17(syb7251[GFP::SL2::unc-17 FLP-on]) IV. | C. elegans | GFP::SL2 FLP-on inserted at N-terminus of endogenous unc-17 locus. Part of SynaptoTagMe toolkit for examining cell-specific cholinergic transmission. See https://elifesciences.org/reviewed-preprints/108675 for sequencing information. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
| DCR9280 | unc-47(syb7313[unc-47::GFP11]) III. | C. elegans | Split GFP11 tag inserted in endogenous unc-47 locus (internal tag). Requires co-expression of complementary GFP1-10. See https://elifesciences.org/reviewed-preprints/108675 for sequencing information. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
| DCR936 | cima-1(wy84) IV; wyIs45 X; olaEx559. | C. elegans | wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx559 [rol-6p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx559 rescues AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. |
| DCR9374 | unc-17(syb7882[unc-17::mRuby3 FLP-on]) IV. | C. elegans | mRuby3 FLP-on inserted at C-terminus of endogenous unc-17 locus. Part of SynaptoTagMe toolkit for examining cell-specific cholinergic transmission. See https://elifesciences.org/reviewed-preprints/108675 for sequencing information. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
| DCR9453 | unc-47(syb7358[unc-47::mKate2]) III. | C. elegans | mKate2 tag inserted in endogenous unc-47 locus (internal tag). Full-body expression of UNC-47::mKate2. Part of SynaptoTagMe toolkit for examining cell-specific GABAergic transmission. See https://elifesciences.org/reviewed-preprints/108675 for sequencing information. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
| DCR9575 | eat-4(syb8568[eat-4::GFP FLP-on]) III. | C. elegans | GFP FLP-on inserted at C-terminus of endogenous eat-4 locus. Part of SynaptoTagMe toolkit for examining cell-specific glutamatergic transmission. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
| DCR9643 | inx-1(tm3524) X; olaIs72; olaEx5670. | C. elegans | inx-1(tm3524) X; olaIs72 [elt-7p::mCherry + ttx-3Gp::SL2::Cx36::mCherry]; olaEx5670 [ttx-3p::GFP + unc-122p::GFP]. Pick animals with GFP+ expression in coelomocytes to maintain. Vertebrate connexin 36 (Cx36) expressed in AIY under control of cell-specific promoter fragment ttx-3G. Suppresses the isothermal tracking phenotype of inx-1. Less membrane resistance than inx-1 mutant, and similar to wild-type. Reference: Almoril-Porras A, et al. Cell. 2025 Jan 9;188(1):89-103.e13. doi: 10.1016/j.cell.2024.11.037. PMID: 39742807. |
| DCR9681 | eat-4(syb9139[eat-4::mRuby3 FLP-on]) III. | C. elegans | mRuby3 FLP-on inserted at C-terminus of endogenous eat-4 locus. Part of SynaptoTagMe toolkit for examining cell-specific glutamatergic transmission. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
| DCR9739 | unc-47(syb7849[unc-47::3xGFP11]) III. | C. elegans | Three copies of split GFP11 tag inserted in endogenous unc-47 locus (internal tag). Requires co-expression of complementary GFP1-10. See https://elifesciences.org/reviewed-preprints/108675 for sequencing information. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
| PHX6275 | unc-25(syb6275 *syb5949) III. | C. elegans | unc-25 coding sequence flanked by FRT sites, allowing cell-specific knockout of unc-25 in a Flippase-dependent manner. Upon recombination, the unc-25 coding sequence is removed and nuclear-localized mCherry marker is expressed. See https://elifesciences.org/reviewed-preprints/108675 for sequencing information. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
| PHX6990 | unc-47(syb6990[unc-47::GFP]) III. | C. elegans | GFP tag inserted in endogenous unc-47 locus (internal tag). Full-body expression of UNC-47::GFP. Part of SynaptoTagMe toolkit for examining cell-specific GABAergic transmission. See https://elifesciences.org/reviewed-preprints/108675 for sequencing information. Reference: Cuentas-Condori A, et al. bioRxiv 2025.08.18.670838; doi: https://doi.org/10.1101/2025.08.18.670838 |
This laboratory hasn't submitted any alleles to the CGC.