Search Strains

kyIs262 [unc-86::myr::GFP + odr-1::RFP] IV. acEx24 [srbc-48p::srbc-48(cDNA) + unc-122::GFP]. Maintain by picking GFP+ to maintain array. Integrated RFP expression in AWB and AWC. Bright GFP expression in coelomocytes. Reference: Kaur S & Aballay A. Cell Reports. 2020 May 19; 31(7): 107662. PMID: 32433971

kyIs262 [unc-86::myr::GFP + odr-1::RFP] IV. acEx25 [odr-1p::srbc-48::GFP]. Maintain by picking GFP+ to maintain array. Extra-chromosomal GFP expression in AWC neurons. Integrated RFP expression in AWB and AWC. acEx25 rescues srbc-48 mediated defects. Reference: Kaur S & Aballay A. Cell Reports. 2020 May 19; 31(7): 107662. PMID: 32433971

Maintain at 15C. Temperature sensitive embryonic lethal. Sterile at 25C. Congenic strain, N2 and Bergerac BE. This allele should NOT be assumed to define a gene to which someone gave the name zyg-12. This name should not be used unless someone finds a non-complementing Bristol mutation. There is no evidence at present that the ts results from a single gene defect. Has been backcrossed >6 times to Bristol strains and should only contain Bergerac DNA in the unc-85 to dpy-10 region.

Unc-stiff anterior.

Migration defective: pre VC. Variable expressivity. Reverse kinker as adult. L1 moves well. Temperature sensitive. Can be maintained at 20C.

Body muscle abnormal. Limp paralyzed or very sluggish; somewhat Egl; larvae more paralyzed. M-MATING-NO SUCCESS.

Slightly slow Unc. Movement almost wild-type. Body muscle abnormal. M-MATING++ 1-10%WT.

Unc-fainter: stops. M-MATING+++ 10-30%WT.

Slow but normal development, disorganized muscle structure, somewhat Egl.

Marginally Unc-cannot back. Lethargic. Migration pre VC defect. M-MATING+POOR <1%WT.

Marginally Unc-kinky backing. Temperature sensitive. Migration pre VC defect. M-MATING+POOR <1%WT. Can be maintained at 20C.

Bursae abnormal. Lineage abnormal. Unc-kinky backing. Unc-lurches forward. Egl. M-MATING-NO SUCCESS. Rec'd new stock from J. Hodgkin 12/4/00.

Egg-laying deficient. Lineage abnormal. Mechanosensory abnormal. M-MATING++ 1-10%WT. Serotonin def.

Unc. Very slow movement. Adults paralyzed. Eggs laid.

Body muscle abnormal. Movement almost WT. Slightly small. M-MATING+++ 10-30%WT.

HSN-. Egl. Mec.

Lon. Unc.

mup-1(e2430) is a temperature sensitive two-fold arrest lethal. Maintain at 15C. Easily scored, at restrictive temperature they look like tiny little croissants. Muv and Unc.

Unc hermaphrodites and males. Mate have variably abnormal tail anatomy. ME 0.

kyIs262 [unc-86::myr::GFP + odr-1::RFP] IV.

Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Originally had kyEx648 [unc-86 + syg-1::GFP] in this strain, but the CGC stock does not contain the array.

kyIs235 [unc-86::snb-1::YFP + unc-4p::lin-10::RFP(intron) + odr-1::RFP]. Also known as unc-86::VAMP::YFP. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.

Suppressed Unc.

DpyUnc strain.

Dpy. pag-1(ls2) is recessive and has not visible phenotype by itself. It increases the expression of certain lacZ fusion genes such as lacZmec-7, unc-86lacZ and unc-4unc-76lacZ.

fnEx4 [unc-85::GFP + rol-6(su1006)]. Rollers. GFP highly expressed in embryos, VPNs in L1, Intestine in L2, VPNs in L3-L4. Maintain by picking GFP+ rollers. Reference: Grigsby IF & Finger FP. (2008) Dev Biol 319(1):100-9.

Break points: In(unc-83 C27A7.1 In(unc-23 lon-3)) V. Covered region (Mb) 5.7 (6.5..12.2) Lon Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241.

Break points: In(unc-83 C27A7.1 In(unc-23 lon-3)) V. Covered region (Mb) 5.7 (6.5..12.2) Balancer marked with myo-2p::Venus. Lon Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241.

Break points: In(unc-83 C27A7.1 In(unc-23 lon-3)) V. Covered region (Mb) 5.7 (6.5..12.2) Balancer marked with myo-2p::mCherry. Lon Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241.

Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted Vulva. CGC rec'd new stock from Iva Greenwald 5/2000. The unc-85 dpy-10 chromosome had been lost from this stock; for some reason it was difficult to maintain as a heterozygote. Do not distribute this strain; other labs should request it from the CGC.

Point mutation W257-> stop codon. Disrupts P cell nuclear migration at 25C. This leads to an Egl, Unc worm. P cell nuclear migration is normal at 15C. hyp7 nuclear migration is normal at all temperatures.

Unc. Muv.

Him. Lethargic. Mec. Egl.

Unc. Egl. Non-Him.

Unc. Non-him.

Unc.

Unc-Lethargic. Mec. Egl. ts Dpy. n847 has a Him phenotype.

Unc. Temperature sensitive allele.

HSN-. Egl. Mec.

Muv. Unc.

Bloated. Mec. Egl. Him.

Blistered Uncs.

Segregates Unc-86 animals and dead eggs.

otIs337 [unc-86(fosmid)::NLS:::YFP::H2B + ttx-3::mCherry].

otIs429 [pag-3(fosmid)::mChOpti + ttx-3::GFP]. otIs337 [unc-86(fosmid)::NLS:::YFP::H2B + ttx-3::mCherry].

otEx5851 [unc-86(fosmid)::NLS::mChopti + lin-44::YFP]. Pick YFP+ to maintain.