Search Strains

stIs12013 [Y48E1B.7.1::H1-wCherry + unc-119(+)].

stIs12022 [ZK909.4::H1-wCherry + unc-119(+)].

stIs12024 [ZK867.1c::H1-wCherry + unc-119(+)].

stIs12061 [mxl-3::H1-wCherry + unc-119(+)].

stIs12063 [Y5F2A.4::H1-wCherry + unc-119(+)].

stIs12065 [B0414.2::H1-wCherry + unc-119(+)].

ceh-20(st12164[ceh-20::GFP + loxP + unc-119(+) + loxP].

zfh-2(st12167[zfh-2::GFP + loxP + unc-119(+) + loxP] I.

ceh-10(st12170[ceh-10::GFP + loxP + unc-119(+) + loxP]) III.

egl-44(st12173[egl-44::GFP+loxP+unc-119(+) loxP]) II.

hlh-26(st12174[hlh-26::GFP + loxP + unc-119(+) + loxP] II.

ast-1(st12175[ast-1::GFP + loxP + unc-119(+) + loxP] II.

lin-26(st12178[lin-26::GFP + loxP + unc-119(+) + loxP] II.

Vul and ts Unc. Maintain at 15C. Becomes sterile at 20C.

stIs20022 [Cbr-his-72::GFP::pie-1 3'UTR + Cbr-unc-119(+)]. C. briggsae with fluorescently labeled (GFP) histones. Reference: Zhou Z, et al. Genetics. 2010 Mar; 184(3): 853–863. doi: 10.1534/genetics.109.110270 PMID: 20008572.

stIs20025 [Cbr-his-72::mCherry::pie-1 3'UTR + Cbr-unc-119(+)]. C. briggsae with fluorescently labeled (mCherry) histones. Reference: Zhou Z, et al. Genetics. 2010 Mar; 184(3): 853–863. doi: 10.1534/genetics.109.110270 PMID: 20008572.

stIs20032 [Cbr-myo-2p::GFP + Cbr-unc-119(+)] V. Transgene inserted into RW20000 Cbr-unc-119. Insertion site is between 0.14 and 4.32 Mb on Chromosome V. Reference: Bi Y, et al. PLoS Genet. 2015 Feb 18;11(2):e1004993.

stIs20105 [Cbr-myo-2p::GFP + Cbr-unc-119(+)] IV. Transgene inserted into RW20000 Cbr-unc-119. Insertion site is between 5.64 and 12.05 Mb on Chromosome IV. Reference: Bi Y, et al. PLoS Genet. 2015 Feb 18;11(2):e1004993.

stIs20107 [Cbr-myo-2p::GFP + Cbr-unc-119(+)] X. Transgene inserted into RW20000 Cbr-unc-119. Insertion site is between 6.02 and 11 Mb on Chromosome X. Reference: Bi Y, et al. PLoS Genet. 2015 Feb 18;11(2):e1004993.

stIs20116 [Cbr-myo-2p::GFP + Cbr-unc-119(+)] V. Transgene inserted into RW20000 Cbr-unc-119. Insertion site is between 12.03 and 17.28 Mb on Chromosome V. Reference: Bi Y, et al. PLoS Genet. 2015 Feb 18;11(2):e1004993.

stIs20120 [Cbr-myo-2p::GFP + Cbr-unc-119(+)] X. Transgene inserted into RW20000 Cbr-unc-119. Insertion site is between 1.59 and 5.19 Mb on Chromosome X. Reference: Bi Y, et al. PLoS Genet. 2015 Feb 18;11(2):e1004993. Yan, Cheung, et al. PLoS ONE 2012 Aug 31 7(8): e43770.

stIs20127 [Cbr-myo-2p::GFP + Cbr-unc-119(+)] X. Transgene inserted into RW20000 Cbr-unc-119. Insertion site is between 4.51 and 5.51 Mb on Chromosome X. Reference: Bi Y, et al. PLoS Genet. 2015 Feb 18;11(2):e1004993.

stIs20133 [Cbr-myo-2p::GFP + Cbr-unc-119(+)] V. Transgene inserted into RW20000 Cbr-unc-119. Insertion site is between 0 and 0.61 Mb on Chromosome V. Reference: Bi Y, et al. PLoS Genet. 2015 Feb 18;11(2):e1004993.

stIs20134 [Cbr-myo-2p::GFP + Cbr-unc-119(+)] V. Transgene inserted into RW20000 Cbr-unc-119. Insertion site is between 8.98 and 12.03 Mb on Chromosome V. Reference: Bi Y, et al. PLoS Genet. 2015 Feb 18;11(2):e1004993.

stIs20144 [Cbr-myo-2p::GFP + Cbr-unc-119(+)] V. Transgene inserted into RW20000 Cbr-unc-119. Insertion site is between 4.66 and 7.75 Mb on Chromosome V. Reference: Bi Y, et al. PLoS Genet. 2015 Feb 18;11(2):e1004993.

Heterozygotes are WT and segregate WT, DpyUnc and Pats. Maintain by picking WT and checking for correction segregation of progeny.

Heterozgyotes are WT and segregate WT, Unc and Pats. Maintain by picking WT and checking for correct segregation of progeny.

ts Unc. At 15C the animals are nearly WT, until adulthood when they become sluggish. At 20C and 25C a gradual deterioration in the animal's ability to move occurs, until as adults they are almost completely paralyzed. They are sterile at 25C.

Segregates WT and Unc. Maintain by picking WT.

tjIs1[pie-1::GFP::rho-1 + unc-119(+)]. GFP-RHO-1 is found at the basement membrane location in germline and early embryos and at the anterior cortical region in one-cell embyros. Small brood size. pID3.01-rho-1. Integration site unknown.

tjIs4 [pie-1::GFP::ect-2 + unc-119(+)]. GFP-ECT-2 is found at the anterior cortex in early embyros. pID3.01-ect-2. Integration site unknown. Maintain at 25C to avoid transgene silencing.

tjIs5 [pie-1p::GFP::plk-1 + unc-119(+)]. GFP::PLK-1 is found at the mitotic centrosomes, kinetochores, and midbody in early embyros. pID3.01-plk-1. Integration site unknown. Maintain at 25C to avoid transgene silencing.

tjIs6 [pie-1p::GFP::cdc-42 + unc-119(+)]. GFP::CDC-42 localizes to basement membranes in both embryos and germline, and to the anterior cortex of one-cell embryos. pID3.01-cdc-42. Integration site unknown.

tjIs8 [pie-1p::GFP::ebp-1 + unc-119(+)]. GFP::ebp-1 is found at the tips of growing microtubules in early embryos. pID3.01-ebp-1. Integration site unknown.

tjIs54 [pie-1p::GFP::tbb-2 + pie-1p::2xmCherry::tbg-1 + unc-119(+)]. tjIs57 [pie-1p::mCherry::his-48 + unc-119(+)]. Maintain at 25C to avoid transgene silencing. Reference: Toya M, et al. Methods Cell Biol. 2010;97:359-72.

ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10047 [unc-14p::his-24::mCherry + unc-119(+)]. Reference: Liu, X, et al., Cell (2009).

ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10035 [eft-3p::HIS-24::mCherry + unc-119(+)]. Reference: Liu, X, et al., Cell (2009).

ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10077 [pha-4p(I2L)::his-24::mCherry + unc-119(+)]. Reference: Liu, X, et al., Cell (2009).

ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10088 [hlh-1p::his-24::mCherry + unc-119(+)]. Reference: Liu, X, et al., Cell (2009).

ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10079 [unc-54p::his-24::mCherry::let-858 3' UTR + unc-119(+)]. Published in Liu, X., et al., Cell, 2009. 139(6).

gaIs209 [sod-3p::his-24::mCherry + unc-119(+)]. Reference: Liu, X, et al., Cell (2009).

gaIs217 [F11E6.3p::HIS-24::mCherry + unc-119(+)]. Published in Liu, X., et al., Cell, 2009. 139(6).

gaIs218 [Y57A10C.6p::his-24::mCherry + unc-119(+)]. Published in Liu, X., et al., Cell, 2009. 139(6).

gaIs220 [col-93p::HIS-24::mCherry + unc-119(+)]. Reference: Liu, X, et al., Cell (2009).

ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10115 [egl-5p::HIS-24::mCherry + unc-119(+)]. Reference: Liu, X, et al., Cell (2009).

gaIs212 contains [F28B4.3p::his-24::mCherry + unc-119(+)]. Published in Liu, X., et al., Cell, 2009. 139(6).

ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. gaIs218 [Y57A10C.6p::HIS-24::mCherry + unc-119(+)]. Reference: Liu, X, et al., Cell (2009).

ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. gaIs222 [C54D10.3p::his-24::mCherry + unc-119(+)]. Reference: Liu, X, et al., Cell (2009).

gaIs228 [C26B9.5p::his-24::mCherry + unc-119(+)]. Published in Liu, X., et al., Cell, 2009. 139(6).

gaIs230 [mif-2p::HIS-24::mCherry + unc-119(+)]. Published in Liu, X., et al., Cell, 2009. 139(6).