| OH11025 |
C. elegans |
otIs252; otEx4965. Show Description
otEx4965 [tbx-38p::mCherry::unc-54 3'UTR + ttx-3::mCherry]. Maintain otEx4965 by picking animals with mCherry in the AIY neurons. otIs252 [lsy-6(fosmid)::YFP + rol-6(su1006)]. Rollers. YFP expression in ASEL.
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| OH11030 |
C. elegans |
otIs379 otIs317. Show Description
otIs379 [cho-1(-3006/-2642)::GFP + rol-6(su1006)]. otIs317 [mgl-1::mCherry + pha-1(+)]. otIs379 appears to be linked to otIs317. Rollers.
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| OH11053 |
C. elegans |
ntIs1 otIs305 otIs355 V. Show Description
ntIs1 [gcy-5p::GFP + lin-15(+)] V. otIs305 [hsp-16.2p::che-1::3xHA::BLRP + rol-6(su1006)] V. otIs355 [rab-3::tagRFP] V. Maintain at 15-20C. Rollers. Reference: Tursun B, et al. Science. 2011 Jan 21;331(6015):304-8.
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| OH11054 |
C. elegans |
pha-1(e2123) III; him-8(e1489) IV; ntIs1 V; otIs305; otEx4445. Show Description
ntIs1 [gcy-5p::GFP + lin-15(+)] V. otIs305 [hsp-16.2p::che-1::3xHA::BLRP + rol-6(su1006)]. otEx4445 [snb-1::NLS::RFP + pBX]. Rollers. Maintain by picking RFP+ Rollers. Maintain at 20C to maintain extrachromosomal array. Reference: Tursun B, et al. Science. 2011 Jan 21;331(6015):304-8.
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| OH11061 |
C. elegans |
otIs381 V. Show Description
otIs381 [ric-19p(prom6)::2xNLS::GFP + elt-2::DsRed] V. Pan-neuronal GFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
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| OH11092 |
C. elegans |
pha-1(e2123) III; otEx5012. Show Description
otEx5012 [lsy-6p::YFP::unc-54 3'UTR + ttx-3p::mCherry + pha-1(+)]. Maintain at 25C; pick mCherry(+).
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| OH11094 |
C. elegans |
pha-1(e2123) III; otEx5014. Show Description
otEx5014 [lsy-6p::YFP::lsy-6 3' sequence + ttx-3p::mCherry + pha-1(+)]. Maintain at 25C; pick mCherry(+).
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| OH11096 |
C. elegans |
pha-1(e2123) III; otEx5016. Show Description
otEx5016 [lsy-6 300bp 3'::lsy-6p::YFP::unc-54 3'UTR + ttx-3p::mCherry + pha-1(+)]. Maintain at 25C; pick mCherry(+). Some rescued worms do not have ttx-3::mCherry expression.
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| OH11098 |
C. elegans |
pha-1(e2123) III; otEx5018. Show Description
otEx5018 [lsy-6p::YFP::lsy-6(300bp 3')::unc-54 3'UTR + ttx-3:mCherry + pha-1(+)]. Maintain by picking animals with mCherry in the AIY neurons. Maintain at 25C to select for pha-1(+) array.
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| OH11101 |
C. elegans |
otEx5021. Show Description
otEx5021 [lsy-6(fosmid - delta 300 bp 3')::GFP + ttx-3::mCherry]. Maintain by picking animals with mCherry expression in the AIY neurons.
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| OH11102 |
C. elegans |
lsy-6(ot71) otIs3 V; otEx5022. Show Description
otEx5022 [lsy-6(fosmid - delta 150 bp 3') + ttx-3::mCherry]. otIs3 [gcy-7p::GFP + lin-15(+)] V. Maintain by picking animals with mCherry expression in the AIY neurons. Fosmid with 150 bp deletion does not rescue ASE asymmetry.
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| OH11104 |
C. elegans |
lsy-6(ot71) otIs3 V; otEx5024. Show Description
otEx5024 [lsy-6(fosmid) + ttx-3::mCherry]. Maintain otEx5024 by picking animals with mCherry in the AIY neurons. otIs3 [gcy-7p::GFP + lin-15(+)] V. Integrated from adEx1288; genetically mapped between 3.05 m.u. (T19B10) and 5.86 m.u. (AH10) on V. GFP expression appears in ASEL and the excretory cell in adult animals.
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| OH11107 |
C. elegans |
otEx5027. Show Description
otEx5027 [lsy-6(fosmid - delta 3 kb 3')::YFP + ttx-3::mCherry]. Maintain by picking animals with mCherry expression in the AIY neurons.
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| OH11111 |
C. elegans |
lsy-6(ot71) otIs3 V; otEx5028. Show Description
otIs3 [gcy-7p::GFP + lin-15(+)] V. otEx5028 [lsy-6p::lsy-6(hairpin) + ttx-3::mCherry].
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| OH11113 |
C. elegans |
lsy-6(ot71) otIs3 V; otEx5030. Show Description
otIs3 [gcy-7p::GFP + lin-15(+)] V. otEx5030 [lsy-6p::lsy-6(hairpin)::lsy-6 1kb 3' + ttx-3::mCherry].
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| OH11115 |
C. elegans |
otIs286. Show Description
otIs386 [lsy-6(fosmid - delta 150 bp 3')::GFP + ttx-3::mCherry].
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| OH11117 |
C. elegans |
otIs306, otEx4963. Show Description
otEx4963 [lsy-6p(fosmid delta 150bp downstream)::GFP + ttx-3:mCherry]. Maintain otEx4963 by picking animals with mCherry in the AIY neurons. otIs306 [hsp-16.2::che-1::3xHA + rol-6(su1006)]. Rollers.
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| OH11119 |
C. elegans |
otIs377; otEx4945. Show Description
otIs377 [myo-3p::mCherry]. otEx4945 [hsp16-2p::hlh-1::2xFLAG + rol-6(su1006)]. Rollers. Maintain at 15-20C; pick Rollers. Both otIs377 and otEx4945 were generated as complex arrays with digested bacterial genomic DNA.
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| OH11124 |
C. elegans |
otIs388 pha-1(e2123) III. Show Description
otIs388 [eat-4(fosmid)::SL2::YFP::H2B + pha-1(+)] III. Reference: Serrano-Saiz E, et al. Cell 2013. Oct; 155(3):659-73.
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| OH11152 |
C. elegans |
otIs392. Show Description
otIs392 [eat-4(prom6)::GFP + ttx-3::DsRed]. Reference: Serrano-Saiz E, et al. Cell 2013. Oct; 155(3):659-73.
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| OH11157 |
C.elegans |
pha-1(e2123) III; otIs393. Show Description
otIs393 [ift-20p::NLS::tagRFP + pha-1(+)]. Maintain at 25C to retain array. Pan-sensory neuronal GFP expression. Reference: Masoudi N, et al. PLoS Biol. 2018 Apr 19;16(4):e2004979.
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| OH11166 |
C. elegans |
vab-3(ot569); otIs138. Show Description
otIs138 [ser-2(prom3)::GFP + rol-6(su1006)]. Rollers. Reference: Serrano-Saiz E, et al. Cell 2013. Oct; 155(3):659-73.
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| OH11411 |
C. elegans |
pha-1(e2123) III; otEx5174. Show Description
otEx5174 [unc-31(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for array. Pan-neuronal nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
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| OH11412 |
C. elegans |
pha-1(e2123) III; otEx5175. Show Description
otEx5175 [maco-1(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for array. Pan-neuronal nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
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| OH11416 |
C. elegans |
otIs419. Show Description
otIs419 [snb-1p(prom17)::2xNLS::GFP + elt-2::DsRed2]. Ubiquitous GFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
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| OH11462 |
C. elegans |
swsn-2.2(ok3161) I; otEx5094. Show Description
otEx5094 [swsn-2.2p::swsn-2.2::mChopti + ttx-3::GFP]. Pick GFP+ to maintain. ok3161 homozygotes arrest in early larval development. otEx5094 rescues lethality, but animals are still somewhat sick at 25C.
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| OH11496 |
C. elegans |
otEx5208. Show Description
otEx5208 [inx-17(fosmid)::GFP + rol-6(su1006)]. Strain does not roll well, but GFP expression is easily detected. GFP tag inserted into fosmid WRM0619cH12.
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| OH11620 |
C. elegans |
otIs429; otIs337. Show Description
otIs429 [pag-3(fosmid)::mChOpti + ttx-3::GFP]. otIs337 [unc-86(fosmid)::NLS:::YFP::H2B + ttx-3::mCherry].
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| OH11630 |
C. elegans |
lsy-27(ot108) II; ntIs1 V. Show Description
ntIs1 [gcy-5p::GFP + lin-15(+)] V. 2 ASER.
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| OH11738 |
C. elegans |
otIs439. Show Description
otIs439 [lad-2p::GFP + pha-1(+)].
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| OH11746 |
C. elegans |
pha-1(e2123) III; otIs447 IV. Show Description
otIs447 [unc-3p::mCherry + pha-1(+)] IV. DA/DB/VA/VB motor neuron class-specific red fluorescent reporter. Reference: Kerk SY, et al. Neuron 2017 (in press).
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| OH11809 |
C. elegans |
otIs450. Show Description
otIs450 [oig-1(fosmid)::SL2::GFP + rol-6(su1006)]. Rollers. Integrated fosmid-based oig-1 transcritpional reporter.
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| OH12312 |
C. elegans |
otIs388 pha-1(e2123) III; him-5(e1490) V. Show Description
otIs388 [eat-4(fosmid)::SL2::YFP::H2B + pha-1(+)] III. Him. Reference: Serrano-Saiz E, et al. Cell. 2013 Oct 24;155(3):659-73.
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| OH12412 |
C.elegans |
otIs502. Show Description
otIs502 [hlh-2(fosmid)::YFP + myo-3p::mCherry]. Broad embryonic expression; later in development transgene is expressed in DTCs, vulva, and some gland cells in pharynx. Reference: Masoudi N, et al. PLoS Biol. 2018 Apr 19;16(4):e2004979.
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| OH12499 |
C. elegans |
otEx5663. Show Description
otEx5663 [unc-30p::GFP::rab-3::unc-10 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. Synaptic GFP marker in D-type motor neurons.
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| OH12500 |
C. elegans |
otEx5664. Show Description
otEx5664 [oig-1(fosmid)::GFP + ttx-3p::mCherry]. Pick mCherry+ animals to maintain. Fosmid-based oig-1 transcritpional reporter.
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| OH12503 |
C. elegans |
otIs520 X. Show Description
otIs520 [eat-4(prom11)::GFP + ttx-3::mCherry] X. AIM is marked only with GFP, not mCherry. Can be used to isolate AIM by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).
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| OH12541 |
C. elegans |
otIs532. Show Description
otIs532 [cho-1(fosmid)::SL2::NLS::YFP::H2B]. YFP expression in cholinergic neurons. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
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| OH12543 |
C. elegans |
otIs534. Show Description
otIs534 [cho-1(fosmid)::SL2::NLS::YFP::H2B]. YFP expression in cholinergic neurons. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
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| OH12644 |
C. elegans |
otEx5765. Show Description
otEx5765 [lin-14(fosmid)::GFP + ttx-3p::mCherry]. Pick mCherry+ animals to maintain. mCherry might be difficult to see at lower magnification. Fosmid-based lin-14 transcritpional reporter; see Li and Greenwald, Current Biology 2010, Oct 26;20(20):1875-9.
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| OH127 |
C. elegans |
him-5(e1490) V; otIs14. Show Description
otIs14 [zig-3::GFP + rol-6(su1006)]. Rollers.
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| OH12734 |
C. elegans |
unc-86(n846) III; otEx5851. Show Description
otEx5851 [unc-86(fosmid)::NLS::mChopti + lin-44::YFP]. Pick YFP+ to maintain.
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| OH12737 |
C. elegans |
uIs115; otIs14; otEx5853. Show Description
uIs115 [mec-17::RFP]. otIs14 [zig-3::GFP + rol-6(su1006)]. Rollers. otEx5853 [hsp::unc-86 + ttx-3::mCherry]. Temperature-sensitive. Maintain at 15C. Pick ttx-3::mCherry-positive animals to maintain array. Expresses unc-86 ubiquitously upon heat shock.
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| OH1277 |
C. elegans |
otEx669. Show Description
otEx669 [exc-4p::GFP + rol-6(su1006)]. Maintain by picking Rollers. Transcriptional GFP fusion expresses in the excretory cell, excretory pore cell, excretory duct cell, rectal gland cell, hypodermis, seam cells, innerlabial sheath cells, and phasmid sheath cells.
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| OH128 |
C. elegans |
otIs39 II; him-5(e1490) V. Show Description
otIs39 [unc-47(delta)::GFP + lin-15(+)]. Expressed in RMEL/R, AVL, RIS, DVB, PVT and unidentified amphid sensory neuron pair.
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| OH12849 |
C. elegans |
pha-1(e2123) III; otIs356 V; otEx5886. Show Description
otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. otEx5886 [unc-57(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for array. Pan-neuronal nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
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| OH12857 |
C. elegans |
pha-1(e2123) III; otIs356 V; otEx5894. Show Description
otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. otEx5894 [egl-3(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for array. Pan-neuronal nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
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| OH12860 |
C. elegans |
pha-1(e2123) III; otIs356 V; otEx5897. Show Description
otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. Pan-neuronal nuclear RFP expression. otEx5897 [egl-21(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for array. Pan-neuronal nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
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| OH12863 |
C. elegans |
pha-1(e2123) III; otIs356 V; otEx5900. Show Description
otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. otEx5900 [tbb-4(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for array. Neuronal nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
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| OH12884 |
C. elegans |
pha-1(e2123) III; otEx5921. Show Description
otEx5921 [syd-2(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for array. Pan-neuronal nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
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