| PS1009 |
C. elegans |
unc-101(sy237) I; sli-1(sy143) X. Show Description
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| PS1411 |
C. elegans |
let-23(sy1) II; sli-1(sy143) X. Show Description
sli-1 is a silent suppressor of the Vul phenotypes of let-23(lf) mutants. sy1;sy143 animals are Hyperinduced. Do not distribute this strain; other labs should request it from the CGC.
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| PS2728 |
C. elegans |
sli-1(sy143) X. Show Description
Do not distribute this strain; other labs should request it from the CGC.
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| PS746 |
C. elegans |
let-23(sy97) II; sli-1(sy143) X. Show Description
sy143 suppresses sy97 viability from 15% to 100%, P12 -> P11 transformations from 27% to 14% and Vul from 100% to 3%. Do not distribute this strain; other labs should request it from the CGC.
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| PS8630 |
C. elegans |
oac-44(sy1431) I. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of oac-44.
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
left flanking sequence: GTTCTCGGATTCCACTTCTACCCAAACCAGTTTCC
right flanking sequence: CAATGGATACCTCGGAGTGGATCAgttaggtttttc
inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TACCCAAACCAGTTTCCCAA
Method Reference: G3 (Bethesda).
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| PS8632 |
C. elegans |
oac-42(sy1433) V. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of oac-42.
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
left flanking sequence: GCTTGACTTACAAGGAATTCGGGGTCTCGCCATTG
right flanking sequence: CTGCAGTGCTTCTTTATCACTTTTATCCAAAACAA
inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: ATAAAGAAGCACTGCAGCAA
Method Reference: G3 (Bethesda).
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| PS8634 |
C. elegans |
col-135(sy1435) IV. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of col-135.
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
left flanking sequence: CCAGCGCTCGGGTATAATATTCGATATCCCTCCT
right flanking sequence: ATGAACCAAATCGACAATATGGCCCATATTCGAATG
inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TTGTCGATTTGGTTCATAGG
Method Reference: G3 (Bethesda).
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| PS8636 |
C. elegans |
ins-10(sy1437) V. Show Description
Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of ins-10.
Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette).
left flanking sequence: CAAAAAACAATTCTTCTAATCTCATTCTTGCTCCTCGT
right flanking sequence: AACATTGGCTCCCAGAACAAGTGCAGCTTTTCCATTC
inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TTCTGGGAGCCAATGTTACG
Method Reference: G3 (Bethesda).
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