Strain Information
| Name | PS8634 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | col-135(sy1435) IV. |
| Description | Superficially wild-type. CRISPR/Cas9 engineered STOP-IN null mutant of col-135. Universal 43bp-long knock-in insertion with 3-frame stop codon (STOP-IN cassette). left flanking sequence: CCAGCGCTCGGGTATAATATTCGATATCCCTCCT right flanking sequence: ATGAACCAAATCGACAATATGGCCCATATTCGAATG inserted sequence between the two flanking sequence (STOP-IN cassette): GGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAgctagc. sgRNA: TTGTCGATTTGGTTCATAGG Method Reference: G3 (Bethesda). |
| Mutagen | Crispr/Cas9 |
| Outcrossed | xNo |
| Made by | Heenam Park/Mandy Tan |
| Laboratory | PS |
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