Strain Information
Name | OH17918 View On Wormbase |
---|---|
Species | C. elegans |
Genotype | lep-5(ot1215[lep-5p::SL1::tbb-2 5'UTR::GFP::H2B::loxP::sqt-1(d)::hygR::loxP::3xFLAG::tbb-2 3'UTR]) X. |
Description | The Null Transcriptional Reporter (NuTR) cassette was used to remove the lep-5 locus resulting in a null allele and transcriptional reporter driving expression of GFP. The cassette contains the dominant sqt-1(e1350) allele that results in roller animals. Expression of Cre (via crossing into a strain expressing a germline Cre or by injection of a Cre transgene) will result in removal of the selectable markers and result in non-roller animals. Pick Rollers to retain full transgene cassette. Males exhibit typical leptoderan tails. |
Mutagen | Crispr/Cas9 |
Outcrossed | x0 |
Made by | Sandeep Wontakal |
Laboratory | OH |
Reference | n/a |
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