Strain Information
| Name | MAS94 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | mei-1(ct46) unc-13(e1091) I; unc-119(ed3) III; abcIs3. |
| Description | abcIs3 [pie-1p::ebp-2::GFP + unc-119(+)]. Embryonic-lethal at 25ºC due to dominant gain-of-function mutation mei-1(ct46), and Uncoordinated due to unc-13 mutation. Maternal expression of EBP-2::GFP microtubule end-binding protein. EBP-2 encodes an EB1-like protein (end-binding) that, in early embryos, locates to the growing tips of microtubules. A strong fluorescent signal localizes to the centrosomes of early embryos due to a high concentration of polymerizing microtubules. mei-1(ct46)-based ectopic microtubule severing causes mitotic spindle defects in the early embryo. References: Gusnowski EM, Srayko M. J Cell Biol. 2011 Aug 8;194(3):377-86. Tegha-Dunghu J, et al. Methods Mol Biol. 2014;1136:103-16. |
| Mutagen | Bombardment |
| Outcrossed | x0 |
| Made by | Eva Gusnowski |
| Laboratory | MAS |
Sign in
or
register an account if you want to order this strain.