Strain Information

Name JK5657   View On Wormbase
Species C. elegans
Genotypetra-1(q183) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I; III).
DescriptionMaintain at 15C. Heterozygotes are WT, GFP+ in the pharynx and segregate WT (GFP+ in the pharynx), dead eggs (hT2 homozygotes) and non-GFP tra-1(q183) homozygous females. Raise strain at 15C; at 20C many heterozygotes are also feminized. tra-1(q183) is temperature-sensitive for its XX, but not its XO mutant phenotype. At 25C, both q183/q183 and q183/+ are female. At 15C, q183/q183 is still always female, but q183/+ can be hermaphroditic. Maintain by picking fertile GFP worms and checking for correct segregation, since the hT2 balancer is lost at low frequencies.
MutagenNo mutagen
Made byPeggy Kroll-Conner
Laboratory JK
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