Strain Information
| Name | JH3861 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | meg-3(ax4502[meg-3(F705A,Y708A,Y713A, N725A)::OLLAS]) meg-4(ax3052) X. |
| Description | 20% Maternal effect sterility on average, embryonic P granule defect, RNAi insensitivity. Engineered mutations in the endogenous meg-3 locus disrupt the binding and localization of PGL proteins to P granules in the early embryo while MEG-3 itself is still forms an asymmetric cytoplasmic gradient and granules. References: Smith, J. et al. Elife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337. PMID: 27914198 Schmidt H, bioRxiv 2020.10.15.340570 (2020) doi:10.1101/2020.10.15.340570. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Helen Schmidt |
| Laboratory | JH |
| Reference | Schmidt, H., Putnam, A., Rasoloson, D. & Seydoux, G. Coordination of RNA and protein condensation by the P granule protein MEG-3. bioRxiv 2020.10.15.340570 (2020) doi:10.1101/2020.10.15.340570. |
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