Strain Information
| Name | CGC184 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | mir-1829.1(umn89[mir-1829.1p+::SL1::EGL-13NLS::lox2272::mScarlet-I::cMycNLS::Lox511I::let-858 3'UTR::lox2722])/tmC24 [F23D12.4(tmIs1240) unc-9(tm9719)] X. |
| Description | tmIs1240 [myo-2p::venus, X: F23D12.4] X. Nuclear mScarlet-I was inserted in place of the endogenous mir-1829.1 pre-miRNA via CRISPR/CAS9. No mScarlet-I expression is seen under standard growh conditions. Heterozygotes are wild-type GFP+ and segregate wild-type GFP+ heterozygotes, non-GFP umn88 homozygotes (arrest stage/phenotype undertemined) and Mec(Unc) GFP+ (tmC24 homozygotes). Maintain by picking wild-type GFP+. Left Flanking: TGCTAAATCCTTTGTTCAGTTATTATAAGT, Right Flanking: ACATTTTTGGATTGGCAAAGTTGTAGTACT. sgRNA: GTTCAGTTATTATAAGTAAG. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Julie Knott and Marcus L. Vargas |
| Laboratory | CGC |
| Reference | n/a |
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