JDW699 |
col-14(wrd271[col-14::mNG::3xFLAG]) IV. |
Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-14 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
JDW733 |
mlt-8(wrd278[mlt-8::mNG::3xFLAG]) II. |
Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous mlt-8 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
JDW734 |
col-12(wrd279[col-12::mNG::3xFLAG]) V. |
Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-12 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
JDW735 |
col-41(wrd280[col-41::mNG::3xFLAG]) X. |
Modular linker::mNeonGreen::3xFLAG::linker tag inserted 6 bp before the stop codon in the endogenous col-41 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
JDW736 |
clec-180(wrd281[clec-180::mNG::3xFLAG]) IV. |
Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous clec-180 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
JDW737 |
mlt-10(wrd282[mlt-10::mNG::3xFLAG]) II. |
Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous mlt-10 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
JDW740 |
acn-1(wrd285[acn-1::mNG::3xFLAG]) X. |
Modular linker::mNeonGreen::3xFLAG::linker tag inserted internally in the first exon of the endogenous acn-1 locus by CRISPR. Will produce a linker::mNG::3xFLAG::linker fusion after the 32nd amino acid. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
JDW742 |
cpg-7(wrd287[cpg-7:::mNG::3xFLAG]) II. |
Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous cpg-7 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
JDW764 |
col-125(wrd300[col-125::mNG::3xFLAG]) IV. |
Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-125 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
JDW765 |
col-103(wrd301[col-103::mNG::3xFLAG]) IV. |
Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-103 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
JDW766 |
piit-1(wrd302[piit-1::mNG::3xFLAG]) V. |
Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous piit-1 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
JDW769 |
lon-8(wrd305[lon-8::mNG::3xFLAG]) V. |
Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous lon-8 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
JDW708 |
nas-37(wrd106 wrd251[nas-37::mScarlet::2xOLLAS]) X. |
linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous nas-37 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd106. |
JDW780 |
col-125(wrd300 wrd312[col-125::mScarlet::2xOLLAS]) IV. |
linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous col-125 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd300. |
JDW781 |
col-103(wrd301 wrd313[col-103::mScarlet::2xOLLAS]) IV. |
linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous col-103 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd301. |
JDW782 |
piit-1(wrd302 wrd314[piit-1::mScarlet::2xOLLAS]) V. |
linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous piit-1 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd302. |
JDW783 |
ctsa-1.1(wrd303 wrd315[ctsa-1.1::mScarlet::2xOLLAS]) II. |
linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous ctsa-1.1 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd303. |
JDW785 |
lon-8(wrd305 wrd317[lon-8::mScarlet::2xOLLAS]) V. |
linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous lon-8 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd305. |
JDW788 |
col-166(wrd319[col-166::mNG::3xFLAG]) X. |
Modular linker::mNeonGreen::3xFLAG::linker tag inserted internally to produce a translational fusion after amino acid 120 in the endogenous col-166 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
NK3030 |
qySi229 I. |
qySi229 [cdh-3p::lmp-1::mNG] I. MosSCI single copy insertion. Anchor cell specific expression of lysosomal protein lmp-1. Superficially wild-type. |
NK2864 |
qySi180 I. |
qySi180 [lin-29p::GFP::CAAX] I. MosSCI single copy insertion. Anchor cell specific expression of CAAX motif. Superficially wild-type. |
NK3080 |
cpIs91 II; sbp-1(qy94[mNG::sbp-1]) III. |
cpIs91 [lag-2p::2x mKate2::PLC(delta)PH::3xHA::tbb-2 3'UTR LoxN] II. sbp-1 locus endogenously tagged with mNG at the N-terminus. Superficially wild-type. |
NK2636 |
fasn-1(qy98[fasn-1::mNG]) I. |
fasn-1 locus endogenously tagged with mNG at the C-terminus. Superficially wild-type. |
NK2925 |
sms-1(qy198[sms-1::mNG]) IV. |
sms-1 locus endogenously tagged with mNG at the C-terminus. Superficially wild-type. |
NK2935 |
hmgr-1(qy202[mNG::hmgr-1]) III. |
hmgr-1 locus endogenously tagged with mNG at the N-terminus. Superficially wild-type. |
NK3017 |
fce-1(qy215[mNG::fce-1]) I. |
fce-1 locus endogenously tagged with mNG at the N-terminus. Superficially wild-type. |
NK2949 |
qySi205 I. |
qySi205 [lin-29p::icmt-1::mscarlet] I. MosSCI single copy insertion. Anchor cell specific expression of prenylation enzyme icmt-1. Superficially wild-type. |
NK3065 |
qySi205 I; sec-16A.1(qy234[mNG::sec16A.1]) III. |
qySi205 [lin-29p::icmt-1::mscarlet] I. MosSCI single copy insertion for anchor cell specific expression of prenylation enzyme icmt-1 and sec-16A.1 locus endogenously tagged with mNG at the N-terminus. Superficially wild-type. |
NK3066 |
rrf-3(pk1426) II; qyIs221. |
qyIs221 [cdh-3p::GFP::ced-10]. Maintain at 20C or lower. |
NK3055 |
qySi147 I; sec-16A.1(qy234[mNG::sec16A.1]) III. |
qySi147 [lin-29p::mKate2::PLC(delta)PH] I. qy234 [mNG::sec16A.1] III. sec16A.1 locus endogenously tagged with mNG at the N-terminus and MosSCI single copy insertion for anchor cell specific expression of membrane marker PLCδPH. Superficially wild-type. |
NK2943 |
hmgr-1(qy202[mNG::hmgr-1]) III; unc-6(ev400) X. |
hmgr-1 locus endogenously tagged with mNG at the N-terminus in unc-6 mutant background. |
NK2966 |
qySi205 I; unc-6(ev400) X. |
qySi205 [lin-29p::icmt-1::mScarlet] I. unc-6 mutant expressing anchor cell specific prenylation enzyme icmt-1. |
NK2929 |
fnta-1(qy199[fnta-1::mNG]) IV. |
fnta-1 locus endogenously tagged with mNG at the C-terminus. Superficially wild-type. |
NK2959 |
rrf-3(pk1426) II; sms-1(qy198[sms-1::mNG]) IV. |
Maintain at 20C or lower. sms-1 locus endogenously tagged with mNG at the C-terminus in rrf-3 mutant background. |
NK2756 |
qyIs562. |
qyIs562 [zmp-1p::zmp-1sp::sfGFP::KDEL]. Anchor cell specific expression of KDEL ER lumen marker. Superficially wild-type. |
NK2990 |
qySi205; qyIs562. |
qySi205 [lin-29p::icmt-1::mscarlet] I. qyIs562 [zmp-1p::zmp-1sp::sfGFP::KDEL]. MosSCI single copy insertion for anchor cell specific expression of prenylation enzyme icmt-1 and anchor cell specific expression of KDEL ER lumen marker. Superficially wild-type. |
NK2752 |
qyIs555. |
qyIs555 [cdh-3p::aman-2::GFP]. Anchor cell specific expression of golgi protein aman-2. Superficially wild-type. |
CHS1001 |
srh-99(yum1120) srh-100(yum1121) II. |
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. |
CHS1002 |
srh-105(yum1122) II; srh-109(yum1123) srh-111(yum1124) V. |
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. |
CHS1003 |
srh-105(yum1122) II; srh-104(yum1126) srh-102(yum1125) srh-109(yum1123) srh-111(yum1124) V. |
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. |
CHS1004 |
srh-141(yum1127) srh-142(yum1128) srh-145(yum1129) V. |
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. |
CHS1006 |
srh-190(yum1133) srh-192(yum1134) srh-193(yum1135) V. |
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. |
CHS1007 |
srh-203(yum1136) srh-204(yum1137) srh-206(yum1138) V. |
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. |
CHS1008 |
srh-213(yum1140) srh-212(yum1139) srh-218(yum1141) V. |
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. |
CHS1009 |
srh-297(yum1144) II; srh-295(yum1142) srh-296(yum1143) V. |
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. |
CHS1010 |
str-112(yum1145) str-113(yum1146) str-114(yum1147) str-115(yum1148) str-116(yum1149) str-118(yum1150) V. |
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. |
CHS1011 |
sri-1(yum1151) sri-2(yum1152) sri-4(yum1153) V. |
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. |
CHS1012 |
srbc-61(yum1154) srbc-62(yum1155) srbc-63(yum1156) srbc-65(yum1157) V. |
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. |
CHS1013 |
sro-1(yum1158) II. |
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. |
CHS1014 |
sru-47(yum1163) I; sru-7(yum1159) IV; c45h4.18(yum1162) sru-44(yum1160) sru-45(yum1161) V; sru-48(yum1164) X. |
Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. |