| MOS88 |
him-5(e1490) V; etyIs1. |
etyIs1 [gpa-13p::FLPase + sra-6p::FRT::mCherry::StopCodon::FRT::GCaMP6s]. Him. Integrated ASH-specific calcium sensor transgene. Reference: Pechuk V., et al. 2022, Current Biology 32, 1–14. https://doi.org/10.1016/j.cub.2022.08.038 |
| MOS370 |
him-5(e1490) V; lite-1(ce314) X; fsIs22; ljIs114. |
fsIs22 [osm-5p::fem-3::mCherry + unc-122p::GFP]. ljIs114 [gpa-13p∷FLPase + sra-6p∷FRT::mCherry::StopCodon::FRT∷ChR2∷YFP]. Him. Pan-ciliated masculinization with ChR2 specifically in ASH. Reference: Pechuk V., et al. 2022, Current Biology 32, 1–14. https://doi.org/10.1016/j.cub.2022.08.038 |
| MOS374 |
him-5(e1490) V; etyEx106. |
etyEx106 [sra-6p::Cx36::YFP + flp-18p::Cx36::mCherry + unc-122p::GFP]. Pick animals with GFP+ coelomocytes to maintain. Artificial gap-junction between ASH and AVA. Reference: Pechuk V., et al. 2022, Current Biology 32, 1–14. https://doi.org/10.1016/j.cub.2022.08.038 |
| JMC201 |
alg-1(tor137[GFP::3xFLAG::alg-1b]) X. |
GFP and 3xFLAG tags inserted into endgonenous alg-1 locus; specifically tags b-isoform. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC203 |
alg-2(tor139[GFP::3xFLAG::alg-2b]) II. |
GFP and 3xFLAG tags inserted into endgonenous alg-2 locus; specifically tags b-isoform. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC205 |
alg-3(tor141[GFP::3xFLAG::alg-3]) IV. |
GFP and 3xFLAG tags inserted into endgonenous alg-3 locus. Reference: Charlesworth AG, et al. Nucleic Acids Res. 2021 Sep 7;49(15):8836-8865. PMID: 34329465 |
| JMC207 |
alg-4(tor143[GFP::3xFLAG::alg-4]) III. |
GFP and 3xFLAG tags inserted into endgonenous alg-4 locus. Reference: Charlesworth AG, et al. Nucleic Acids Res. 2021 Sep 7;49(15):8836-8865. PMID: 34329465 |
| JMC209 |
alg-5(tor145[GFP::3xFLAG::alg-5]) I. |
GFP and 3xFLAG tags inserted into endgonenous alg-5 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC211 |
ergo-1(tor147[GFP::3xFLAG::ergo-1a]) V. |
GFP and 3xFLAG tags inserted into endgonenous ergo-1 locus; specifically tags a-isoform. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC213 |
prg-1(tor149[GFP::3xFLAG::prg-1]) I. |
GFP and 3xFLAG tags inserted into endgonenous prg-1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC217 |
rde-1(tor153[GFP::3xFLAG::rde-1]) V. |
GFP and 3xFLAG tags inserted into endgonenous rde-1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC219 |
wago-1(tor113[GFP::3xFLAG::wago-1]) I. |
GFP and 3xFLAG tags inserted into endgonenous wago-1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC273 |
wago-1(tor163[3xFLAG::wago-1] |
3xFLAG tag inserted into endgonenous wago-1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC221 |
ppw-2(tor115[GFP::3xFLAG::ppw-2]) I. |
GFP and 3xFLAG tags inserted into endgonenous ppw-2 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC223 |
wago-4(tor117[GFP::3xFLAG::wago-4]) II. |
GFP and 3xFLAG tags inserted into endgonenous wago-4 locus. Reference: Lev I, et al. Curr Biol. 2019 Sep 9;29(17):2880-2891.e4. PMID: 31378614 |
| JMC225 |
ppw-1(tor119[GFP::3xFLAG::ppw-1c]) I. |
GFP and 3xFLAG tags inserted into endgonenous ppw-1; specifically tags c-isoform. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC227 |
sago-2(tor121[GFP::3xFLAG::sago-2c]) I. |
GFP and 3xFLAG tags inserted into endgonenous sago-2; specifically tags c-isoform. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC229 |
sago-1(tor123[GFP::3xFLAG::sago-1]) V. |
GFP and 3xFLAG tags inserted into endgonenous sago-1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC231 |
hrde-1(tor125[GFP::3xFLAG::hrde-1]) III. |
GFP and 3xFLAG tags inserted into endgonenous hrde-1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC233 |
wago-10(tor127[GFP::3xFLAG::wago-10]) V. |
GFP and 3xFLAG tags inserted into endgonenous wago-10 locus. Reference: Charlesworth AG, et al. Nucleic Acids Res. 2021 Sep 7;49(15):8836-8865. PMID: 34329465 |
| JMC248 |
wago-10(tor162[3xFLAG::wago-10] V. |
3xFLAG tag inserted into endgonenous wago-10 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC235 |
wago-11(tor129[GFP::3xFLAG::wago-11]) II. |
GFP and 3xFLAG tags inserted into endgonenous wago-11 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC237 |
nrde-3(tor131[GFP::3xFLAG::nrde-3]) X. |
GFP and 3xFLAG tags inserted into endgonenous nrde-3 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC247 |
nrde-3(tor134[3xFLAG::nrde-3]) X. |
3xFLAG tag inserted into endgonenous nrde-3 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC193 |
sago-2(tor135) I. |
Null allele of sago-2; detectable by PCR/restriction digest. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC192 |
wago-10(tor133) V. |
Null allele of wago-10. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC151 |
csr-1(tor160[csr-1 exon1::GFP::FLAG (IV:7957568)]) IV . |
GFP and 3xFLAG tags inserted into first exon of endgonenous csr-1 locus (IV:7957568); specifically tags a-isoform. |
| JMC164 |
csr-1(tor67[csr-1 exon2::GFP::FLAG IV:7958598]csr- 1(mg660[G120*]) IV) IV . |
Null allele of csr-1 with GFP and 3xFLAG tags inserted into second exon of endgonenous csr-1 locus. tor67 would normally tag both long and short isoforms, but the mg660 allele introduces a stop codon into the first exon so the long isoform is not made and only tagged CSR-1B isoform is produced. |
| JMC101 |
csr-1(tor67[gfp::3xflag::csr-1]) IV . |
GFP and 3xFLAG tags inserted into second exon of endgonenous csr-1 locus; tags both isoforms. Reference: Ouyang JPT, et al. Dev Cell. 2019 Sep 23;50(6):716-728.e6. PMID: 31402283 |
| JMC135 |
vsra-1(tor94[GFP::3xFLAG::vsra-1] I. |
GFP and 3xFLAG tags inserted into endgonenous vsra-1/C04F12.1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC327 |
vsra-1(tor170[3xflag::vsra-1]) I. |
3xFLAG tag inserted into endgonenous vsra-1/C04F12.1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| JMC245 |
alg-4(tm1184) III; csr-1(tor67[csr-1 exon2::gfp::3xflag (IV:7958598)], csr-1(mg660[G120*])) alg-3(tm1155) IV; wago-10(tor133) V. |
Quadruple mutant of four spermatogenesis-specific ago genes. Reference: Charlesworth AG, et al. Nucleic Acids Res. 2021 Sep 7;49(15):8836-8865. PMID: 34329465 |
| JMC288 |
wago-5(tor166[GFP::3xFLAG::wago-5]) II. |
GFP and 3xFLAG tags inserted into endgonenous wago-5 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 |
| OH17019 |
otIs825. |
otIs825 [degl-1p::GFP + unc-122p::GFP]. Reference: Cros C & Hobert O. bioRxiv 2022.04.19.488792; doi: https://doi.org/10.1101/2022.04.19.488792. |
| OH17217 |
otIs846. |
otIs846 [egas-1p::GFP + unc-122p::GFP]. Reference: Cros C & Hobert O. bioRxiv 2022.04.19.488792; doi: https://doi.org/10.1101/2022.04.19.488792. |
| PHX4447 |
aex-2(syb4447 [aex-2::SL2::GFP::H2B)] X. |
CRISPR/Cas9 engineered tagged endogenous locus. Reference: Cros C & Hobert O. Proc Natl Acad Sci USA. 2022 Sep 13;119(37):e2206817119. doi: 10.1073/pnas.2206817119. PMID: 36067313. |
| PHX4514 |
dmsr-2(syb4514 [dmsr-2::SL2::gfp::H2B]) I. |
CRISPR/Cas9 engineered tagged endogenous locus. Reference: Cros C & Hobert O. Proc Natl Acad Sci USA. 2022 Sep 13;119(37):e2206817119. doi: 10.1073/pnas.2206817119. PMID: 36067313. |
| PHX4693 |
che-7(syb4693[che-7::SL2::gfp::H2B]) V. |
CRISPR/Cas9 engineered tagged endogenous locus. Reference: Cros C & Hobert O. bioRxiv 2022.04.19.488792; doi: https://doi.org/10.1101/2022.04.19.488792. |
| PHX5452 |
ins-1(syb5452[ins-1::SL2::gfp::H2B]) IV. |
CRISPR/Cas9 engineered tagged endogenous locus. Reference: Cros C & Hobert O. bioRxiv 2022.04.19.488792; doi: https://doi.org/10.1101/2022.04.19.488792. |
| PHX5229 |
degl-2(syb5229[degl-2::SL2::gfp::H2B]) IV. |
CRISPR/Cas9 engineered tagged endogenous locus. |
| PHX3317 |
flp-11b(syb3317[flp-11b::T2A::3XNLS::GFP]) X |
CRISPR/Cas9 engineered tagged endogenous locus. |
| PHX3242 |
flp-23(syb3242[flp-23::T2A::3xNLS::GFP]) III. |
CRISPR/Cas9 engineered tagged endogenous locus. |
| ZM9128 |
hpIs595. |
hpIs595 [acr-2(s)p::GCaMP6s::wCherry + lin-15(+)]. Red fluorescence in motor neurons. Reference: Lu Y, et al. 2022. Current Biology (In Press). |
| ZM10339 |
hpIs717; ljIs131. |
hpIs717 [acr-2(s)p::LoxP::eBFP::LoxP::Chrimson::wCherry + unc-17p::Cre + lin-15(+)]. ljIs131 [myo-3p::GCaMP3::UrSL2::tagRFP-T]. Red fluorescence in motor neurons. Reference: Lu Y, et al. 2022. Current Biology (In Press). |
| ZM7656 |
hpIs365. |
hpIs365 [unc-25p::GCaMP3::wCherry + lin-15(+)]. RFP expression in motor neurons. Reference: Lu Y, et al. 2022. Current Biology (In Press). |
| ZM9551 |
hpIs593; ljIs131. |
hpIs593 [ttr-39p::Chrimson::wCherry + lin-15(+)]. ljIs131 [myo-3p::GCaMP3::UrSL2::tagRFP-T]. D motor neuron activation and muscle relaxation upon illumination with green light. Muscle activity measured by GCaMP3. Reference: Lu Y, et al. 2022. Current Biology (In Press). |
| ZM9313 |
hpIs625; ljIs131. |
hpIs625 [ttr-39p::Arch::wCherry + lin-15(+)]. ljIs131 [myo-3p::GCaMP3::UrSL2::tagRFP-T]. Reference: Lu Y, et al. 2022. Current Biology (In Press). |
| ZM9172 |
unc-25(e156) III; ljIs131. |
ljIs131 [myo-3p::GCaMP3::UrSL2::tagRFP-T]. Shrinker. Reference: Lu Y, et al. 2022. Current Biology (In Press). |
| ZM10823 |
hpIs596; hpIs268. |
hpIs596 [acr-2(s)p::Chrimson::wCherry + lin-15(+)]. hpIs268 [unc-25p::GCaMP3si::SL2 wCherry + lin-15(+)]. Unc (linked to hpIs596). Green and red fluorescence in D-motor neurons. Very weak red fluorescence in A and B motorneurons. Reference: Lim MA, et al. eLife 2016;5:e19887. doi: 10.7554/eLife.19887. PMID: 27855782. |
| ZM9585 |
hpIs615; hpIs365. |
hpIs615 [acr-2(s)p::Arch::wCherry + lin-15(+)]. hpIs365 [unc-25p::GCaMP3::wCherry + lin-15(+)]. RFP expression in motor neurons. A and B motor neurons are inhibited and body relaxes upon illumination with green light. Reference: Lu Y, et al. 2022. Current Biology (In Press). |