Strain Information
| Name | ZM8614 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | hpIs372; hpIs365. |
| Description | hpIs372 [acr-5p::tomm-20::miniSOG::SL2::wCherry + lin-15(+)]. hpIs365 [unc-25p::GCaMP3::wCherry + lin-15(+)]. Maintain in the covered box to avoid unnecessary exposure to ambient light. Stimulation with blue light (460 nm LED light for 30 min at 4 Hz with 2 mW/mm2) induces mitochondrial-miniSOG ablation of B-class motor neurons. During neuron ablation, it is recommended to keep the lid of the plate open and use a heat dissipator to keep the air cool. Red fluorescence in motor neurons. Reference: Lu Y, et al. 2022. Current Biology (In Press). |
| Mutagen | |
| Outcrossed | x0 |
| Made by | Asuka Guan |
| Laboratory | ZM |
| Reference | Lu Y, Ahamed T, Mulcahy B, Witvliet D, Guan SA, Hung W, Meng J, Wen Q, Samuel ADT, Zhen M.*. Extrasynaptic signaling enables an asymmetric juvenile motor circuit to produce a symmetric gait. Current Biology. In Press |
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