Species Information: C. elegans

Name C. elegans
NCBI Taxonomy ID

C. elegans strains available at the CGC

Strain Genotype Description
NM4397 jsIs973 III; ptrn-1(js1286) X. jsIs973 [mec-7p::mRFP + unc-119(+)] III. Strong RFP cytosolic marker for mechanosensory neurons (Zheng et al. 2011, PMID 21115607).
NM4422 jsIs973 III; oxIs12 ptrn-1(tm5597) X. jsIs973 [mec-7p::mRFP + unc-119(+)] III.  oxIs12 [unc-47p::GFP + lin-15(+)] X. oxIs12 integration maps at +2.0 on X. Strong RFP cytosolic marker for mechanosensory neurons (Zheng et al. 2011, PMID 21115607). Expression of GFP in all GABAergic neurons.
DMS303 nIs590 V. nIs590 [fat-7p::fat-7::GFP + lin15(+)] V.  Derived by X-ray integration of waEx15. The fat-7::fat-7::GFP translational reporter is activated by 15C or acdh-11 loss-of-function. See Ma et al., Cell. 2015 May 21; 161(5): 1152–1163.
DMS441 acdh-11(n5878) III; nIs590 V. nIs590 [fat-7p::fat-7::GFP + lin15(+)] V.  The fat-7::fat-7::GFP translational reporter is activated constitutively by loss of acdh-11 function. acdh-11(n5878) is a 366 bp deletion. Reference: Ma et al., Cell. 2015 May 21; 161(5): 1152–1163.
DMS640 nIs470 IV. nIs470 [cysl-2p::GFP + myo-2p::mCherry] IV.  The cysl-2::GFP reporter is activated by HIF-1 in egl-9, rhy-1 or vhl-1 loss-of-function mutants.
KX54 ifg-1(cxTi9279) II; bcIs39 V. bcIs39 [lim-7p::ced-1::GFP and lin-15(+)] V.  Temperature-sensitive germ cell apoptosis leading to infertility at 25 C. Apoptosing germ cells are decorated by CED-1::GFP in the gonad. Loss of p170 form of IFG-1 (but not p130 IFG-1) confirmed by Western blot. Escaping eggs are fertilized and embryonic lethal. Mos-1 transposon insertion previously described as ifg-1::mos-1(cxP9279) II; confirmed by triple primer PCR with 5’-ACCAAACTGGGCAAACAAAG-3’, 5’-GCTCAATTCGCGCCAAACTATG-3’, and 5’-CTTCCTGAAATTTGGTTTAACAGT-3’. Homozygous ifg-1::mos yields only 444 bp product. Outcrossed heterozygotes yield both 353 bp (wild type ifg-1) and 444 bp products.
KX84 ced-3(n2452) IV; bcIs39 V. bcIs39 [lim-7p::ced-1::GFP and lin-15(+)] V.  Resistant to germ cell apoptosis. No apoptosing germ cells are decorated by CED-1::GFP in the gonad. ced-3 deletion confirmed by genomic triple primer PCR with 5’-AGTTCACCGTGACAGCGTCTCTTC-3’, 5’-CGATTACGACTTGAACTGTATCCGA-3’, and 5’-TCTTGTGTAAACGAGATTTGCAATG-3’. Homozygous ced-3(n2452) yields only 1,110 bp product. Outcrossed heterozygotes yield both 1,411 bp (wild type ced-3) and 1,110 bp products. [NOTE: (11-05-2019) A user has reported this strain exhibits temperature-sensitive sterility when raised at 25C.]
KX89 ced-4(n1162) III; bcIs39 V. bcIs39 [lim-7p::ced-1::GFP and lin-15(+)] V.  Resistant to germ cell apoptosis. No apoptosing germ cells are decorated by CED-1::GFP in the gonad. Genetically identical to KX87 (Contreras, V., et al, 2011).
KX110 ced-9(n1653) mab-5(mu14) III; bcIs39 V. bcIs39 [lim-7p::ced-1::GFP and lin-15(+)] V.  Temperature sensitive induction of germ cell apoptosis at 25 C. Large number of germ cells are decorated by CED-1::GFP within 48 h. Activates CED-3-mediated cleavage of IFG-1, visualized by western blot (Contreras, V., et al, 2011).
KP3814 nuIs152 II. nuIs152 [unc-129p::GFP::snb-1 + ttx-3p::mRFP] II.
KP3972 nuIs184 X. nuIs184 [unc-129p::apt-4::GFP + myo-2p::GFP] X.
JK2157 lag-2(q411) V; qEx319. qEx319 [lag-2::cMyc + rol-6(su1006)].  Rollers.  Extrachromosomal array carrying myc-tagged LAG-2 protein partially rescues lag-2(null).  About 70% of progeny are Lag.  Survivors are Rollers.  Reference: Crittenden et al., Mol Biol Cell. 2006 Jul;17(7):3051-61.
DG4105 lfor-1(tn1652) lfor-2(tn1653) II. Apparently wild type
MG589 xsSi3 II. xsSi3 [GFP::utrophin + Cbr-unc-119(+)].  Superficially wild-type.  Carries a germline-expressed actin probe derived from the calponin homology domain of utrophin.  
DG4158 spn-4(tn1699[spn-4::gfp::3xflag]) V. Apparently wild type
KP4211 nuIs214 III. nuIs214 [unc-129p::itsn-1::GFP + myo-2p::GFP] III.
KP5445 nuIs165. nuIs165 [unc-129p::unc-10::GFP + myo-2p::GFP] II.
DG4190 cdc-25.3(tn1712[gfp::3xflag::cdc-25.3]) III. Superficially wild type
DG4213 meg-1(tn1724[gfp::3xflag::meg-1]) X. Superficially wild type
DG4215 puf-5(tn1726[gfp::3xflag::puf-5]) II. Superficially wild type
DG4218 cpg-1(tn1728[mng::3xflag::cpg-1]) III. Superficially wild type
DG4222 pos-1(tn1730[gfp::3xflag::pos-1]) V. Superficially wild type
DG4228 orc-1(tn1732[mNeonGreen::3xflag::orc-1]) III. Superficially wild type
DG4230 gla-3a(tn1734[gfp::3xflag::gla-3a]) I. Superficially wild type
DG4233 pqn-59(tn1737[gfp::3xflag::pqn-59]) I. Superficially wild type
DG4269 mex-3(tn1753[gfp::3xflag::mex-3]) I. Superficially wild type
DG4392 cyb-3(tn1755[gfp::3xflag::cyb-3]) V. Although this strain is maintainable as a homozygote it produces many dead embryos (~80%) and has a low viable brood size (~24 ± 18). Thus, the GFP tag compromises CYB-3 function.
CGC53 mIn1 [dpy-10(e128) umnIs43]/unc-4(e120) II. umnIs43 [myo-2p::mKate2 + NeoR, II: 11755713 (intergenic)] II. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2, Unc-4 non-mKate2, and Dpy mKate2+ mIn1 homozygotes. Maintain by picking wild-type mKate2+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::mKate2 transgene into mIn1 balancer in parental strain DR1785 using CRISPR/Cas9.
CF12 rol-6(e187) II; lin-22(n372) IV; him-5(e1490) V. Rollers. lin-22 and him-5 mutations affect neuroblast formation from epidermal precursor cell V5.
CF26 lin-22(mu2) IV; him-5(e1490) V. Alae-to-ray transformation similar to that of n372 animals.
CF31 lin-22(mu5) IV; him-5(e1490) V. Alae-to-ray transformation similar to that of n372 animals.
CF36 lin-22(n372) unc-33(e204) IV. Desorption
CF65 mab-5(e2088) III; lin-22(n372) IV; him-5(e1490) V. mab-5 mutation affects ectodermal and mesodermal lineages. lin-22 and him-5 mutations affect neuroblast formation from the epidermal precursor cell V5.
CF2164 muEx329. muEx329 [jnk-1::GFP + myo-3p::RFP]. Pick RFP+ to maintain.
CF2805 dop-2(vs105) V; dop-4(ok1321) dop-1(vs100) dop-3(vs106) X Quadruple mutant removing four different dopamine receptor genes.
JN2411 sinh-1(pe420) II. Chemotaxis abnormality, developmental delay, small brood size.  pe420 is a 22 bp deletion in exon 1.
PS6843 syIs300 V. syIs300 [15xUAS::Δpes-10::GFP::unc-54 3'UTR + ttx-3p::RFP + pBlueScript].  GFP cGAL effector.  Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with transgenetic marker in next generation. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.
PS6872 syIs302 III. syIs302 [15xUAS::Δpes-10::GFP::unc-54 3'UTR + ttx-3p::RFP + pBlueScript].  GFP cGAL effector. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.
PS6974 syIs337 III. syIs337 [15xUAS::Δpes-10::GFP::let-858 3'UTR + ttx-3p::RFP + pBluescript].  GFP cGAL effector.  Weak background fluorescence in some head neurons and the head mesodermal cell.  Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.
PS7149 syIs390 X. syIs390 [15xUAS::Δpes-10::GFP::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder(NEB)].  GFP cGAL effector.  Weak background fluorescence in some head neurons and the head mesodermal cell.  [NOTE: (03/18/2020) a user has reported syIs390 does not map to LG X] Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.
PS7136 syIs378 I. syIs378 [15xUAS::Δpes-10::mKate2::let-858 3'UTR + unc-122p::GFP + 1kb DNA ladder(NEB)].  mKate2 cGAL effector.  Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.
PS7185 syIs406 IV. syIs406 [15xUAS::Δpes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript].  GFP::H2B effector.  Very weak background fluorescence in first ring of intestinal cells and posterior intestinal cells.  Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.
PS7186 syIs407 V. syIs407 [15xUAS::Δpes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript].  GFP::H2B cGAL effector.  Very weak background fluorescence in first ring of intestinal cells and posterior intestinal cells.  Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.
PS7190 syIs409 X. syIs409 [15xUAS::Δpes-10::mCherry::H2B::let-858 3'UTR + unc-122p::GFP + pBlueScript].  mCherry::H2B cGAL effector.  Very weak background fluorescence in first ring of intestinal cells and posterior intestinal cells.  Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.
PS7203 syIs423 V. syIs423 [15xUAS::Δpes-10::GCaMP6s::SL2::mKate2::let-858 3'UTR + myo-2p::NLS::mCherry + 1kb DNA ladder(NEB)].  GCaMP6s cGAL effector.  Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.
PS7044 syIs341 IV. syIs341 [15xUAS::Δpes-10::hChR2(Y134R)::YFP::let-858 3'UTR + ttx-3p::RFP + pBlueScript].  channelrhodopsin cGAL effector.  Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.
PS7045 syIs342 II. syIs342 [15xUAS::Δpes-10::hChR2(Y134R)::YFP::let-858 3'UTR + ttx-3p::RFP + pBlueScript].  channelrhodopsin cGAL effector.  Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.
PS7199 syIs371 III. syIs371 [15xUAS::Δpes-10::HisCl1::SL2::GFP::let-858 3'UTR + unc-122p::GFP + 1kb DNA ladder(NEB)].  Histamine chloride channel cGAL effector.  Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.
PS7107 syIs373 I. syIs373 [15xUAS::Δpes-10::HisCl1::SL2::GFP::let-858 3'UTR + unc-122p::GFP + 1kb DNA ladder(NEB)].  Histamine chloride channel cGAL.  Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.
PS7108 syIs374 V. syIs374 [15xUAS::Δpes-10::HisCl1::SL2::GFP::let-858 3'UTR + unc-122p::GFP + 1kb DNA ladder(NEB)].  histamine chloride channel cGAL effector.  Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148.