| NM4397 |
jsIs973 III; ptrn-1(js1286) X. |
jsIs973 [mec-7p::mRFP + unc-119(+)] III. Strong RFP cytosolic marker for mechanosensory neurons (Zheng et al. 2011, PMID 21115607). |
| NM4422 |
jsIs973 III; oxIs12 ptrn-1(tm5597) X. |
jsIs973 [mec-7p::mRFP + unc-119(+)] III. oxIs12 [unc-47p::GFP + lin-15(+)] X. oxIs12 integration maps at +2.0 on X. Strong RFP cytosolic marker for mechanosensory neurons (Zheng et al. 2011, PMID 21115607). Expression of GFP in all GABAergic neurons. |
| DMS303 |
nIs590 V. |
nIs590 [fat-7p::fat-7::GFP + lin15(+)] V. Derived by X-ray integration of waEx15. The fat-7::fat-7::GFP translational reporter is activated by 15C or acdh-11 loss-of-function. See Ma et al., Cell. 2015 May 21; 161(5): 1152–1163. |
| DMS441 |
acdh-11(n5878) III; nIs590 V. |
nIs590 [fat-7p::fat-7::GFP + lin15(+)] V. The fat-7::fat-7::GFP translational reporter is activated constitutively by loss of acdh-11 function. acdh-11(n5878) is a 366 bp deletion. Reference: Ma et al., Cell. 2015 May 21; 161(5): 1152–1163. |
| DMS640 |
nIs470 IV. |
nIs470 [cysl-2p::GFP + myo-2p::mCherry] IV. The cysl-2::GFP reporter is activated by HIF-1 in egl-9, rhy-1 or vhl-1 loss-of-function mutants. |
| KX54 |
ifg-1(cxTi9279)
II; bcIs39 V. |
bcIs39 [lim-7p::ced-1::GFP and lin-15(+)] V. Temperature-sensitive germ cell apoptosis leading to infertility at 25 C. Apoptosing germ cells are decorated by CED-1::GFP in the gonad. Loss of p170 form of IFG-1 (but not p130 IFG-1) confirmed by Western blot. Escaping eggs are fertilized and embryonic lethal. Mos-1 transposon insertion previously described as ifg-1::mos-1(cxP9279)
II; confirmed by triple primer PCR with 5’-ACCAAACTGGGCAAACAAAG-3’, 5’-GCTCAATTCGCGCCAAACTATG-3’, and 5’-CTTCCTGAAATTTGGTTTAACAGT-3’. Homozygous ifg-1::mos yields only 444 bp product. Outcrossed heterozygotes yield both 353 bp (wild type ifg-1) and 444 bp products. |
| KX84 |
ced-3(n2452) IV; bcIs39 V. |
bcIs39 [lim-7p::ced-1::GFP and lin-15(+)] V. Resistant to germ cell apoptosis. No apoptosing germ cells are decorated by CED-1::GFP in the gonad. ced-3 deletion confirmed by genomic triple primer PCR with 5’-AGTTCACCGTGACAGCGTCTCTTC-3’, 5’-CGATTACGACTTGAACTGTATCCGA-3’, and 5’-TCTTGTGTAAACGAGATTTGCAATG-3’. Homozygous ced-3(n2452) yields only 1,110 bp product. Outcrossed heterozygotes yield both 1,411 bp (wild type ced-3) and 1,110 bp products. [NOTE: (11-05-2019) A user has reported this strain exhibits temperature-sensitive sterility when raised at 25C.] |
| KX89 |
ced-4(n1162)
III; bcIs39 V. |
bcIs39 [lim-7p::ced-1::GFP and lin-15(+)] V. Resistant to germ cell apoptosis. No apoptosing germ cells are decorated by CED-1::GFP in the gonad. Genetically identical to KX87 (Contreras, V., et al, 2011). |
| KX110 |
ced-9(n1653)
mab-5(mu14) III; bcIs39 V. |
bcIs39 [lim-7p::ced-1::GFP and lin-15(+)] V. Temperature sensitive induction of germ cell apoptosis at 25 C. Large number of germ cells are decorated by CED-1::GFP within 48 h. Activates CED-3-mediated cleavage of IFG-1, visualized by western blot (Contreras, V., et al, 2011). |
| KP3814 |
nuIs152 II. |
nuIs152 [unc-129p::GFP::snb-1 + ttx-3p::mRFP] II. |
| KP3972 |
nuIs184 X. |
nuIs184 [unc-129p::apt-4::GFP + myo-2p::GFP] X. |
| JK2157 |
lag-2(q411) V; qEx319. |
qEx319 [lag-2::cMyc + rol-6(su1006)]. Rollers. Extrachromosomal array carrying myc-tagged LAG-2 protein partially rescues lag-2(null). About 70% of progeny are Lag. Survivors are Rollers. Reference: Crittenden et al., Mol Biol Cell. 2006 Jul;17(7):3051-61. |
| DG4105 |
lfor-1(tn1652) lfor-2(tn1653) II. |
Apparently wild type |
| MG589 |
xsSi3 II. |
xsSi3 [GFP::utrophin + Cbr-unc-119(+)]. Superficially wild-type. Carries a germline-expressed actin probe derived from the calponin homology domain of utrophin. |
| DG4158 |
spn-4(tn1699[spn-4::gfp::3xflag]) V. |
Apparently wild type |
| KP4211 |
nuIs214 III. |
nuIs214 [unc-129p::itsn-1::GFP + myo-2p::GFP] III. |
| KP5445 |
nuIs165. |
nuIs165 [unc-129p::unc-10::GFP + myo-2p::GFP] II. |
| DG4190 |
cdc-25.3(tn1712[gfp::3xflag::cdc-25.3]) III. |
Superficially wild type |
| DG4213 |
meg-1(tn1724[gfp::3xflag::meg-1]) X. |
Superficially wild type |
| DG4215 |
puf-5(tn1726[gfp::3xflag::puf-5]) II. |
Superficially wild type |
| DG4218 |
cpg-1(tn1728[mNG::3xflag::cpg-1]) III. |
mNeonGreen and Flag tags inserted at 5' end of endogenous cpg-1 locus. Superficially wild type. |
| DG4222 |
pos-1(tn1730[gfp::3xflag::pos-1]) V. |
Superficially wild type |
| DG4228 |
orc-1(tn1732[mNeonGreen::3xflag::orc-1]) III. |
Superficially wild type |
| DG4230 |
gla-3a(tn1734[gfp::3xflag::gla-3a]) I. |
Superficially wild type |
| DG4233 |
pqn-59(tn1737[gfp::3xflag::pqn-59]) I. |
Superficially wild type |
| DG4269 |
mex-3(tn1753[gfp::3xflag::mex-3]) I. |
Superficially wild type |
| DG4392 |
cyb-3(tn1755[gfp::3xflag::cyb-3]) V. |
Although this strain is maintainable as a homozygote it produces many dead embryos (~80%) and has a low viable brood size (~24 ± 18). Thus, the GFP tag compromises CYB-3 function. |
| CGC53 |
mIn1 [dpy-10(e128) umnIs43]/unc-4(e120) II. |
umnIs43 [myo-2p::mKate2 + NeoR, II: 11755713 (intergenic)] II. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2, Unc-4 non-mKate2, and Dpy mKate2+ mIn1 homozygotes. Maintain by picking wild-type mKate2+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::mKate2 transgene into mIn1 balancer in parental strain DR1785 using CRISPR/Cas9. |
| CF12 |
rol-6(e187) II; lin-22(n372) IV; him-5(e1490) V. |
Rollers. lin-22 and him-5 mutations affect neuroblast formation from epidermal precursor cell V5. |
| CF26 |
lin-22(mu2) IV; him-5(e1490) V. |
Alae-to-ray transformation similar to that of n372 animals. |
| CF31 |
lin-22(mu5) IV; him-5(e1490) V. |
Alae-to-ray transformation similar to that of n372 animals. |
| CF36 |
lin-22(n372) unc-33(e204) IV. |
Desorption |
| CF65 |
mab-5(e2088) III; lin-22(n372) IV; him-5(e1490) V. |
mab-5 mutation affects ectodermal and mesodermal lineages. lin-22 and him-5 mutations affect neuroblast formation from the epidermal precursor cell V5. |
| CF2164 |
muEx329. |
muEx329 [jnk-1::GFP + myo-3p::RFP]. Pick RFP+ to maintain. |
| CF2805 |
dop-2(vs105) V; dop-4(ok1321) dop-1(vs100) dop-3(vs106) X |
Quadruple mutant removing four different dopamine receptor genes. |
| JN2411 |
sinh-1(pe420) II. |
Chemotaxis abnormality, developmental delay, small brood size. pe420 is a 22 bp deletion in exon 1. |
| PS6843 |
syIs300 V. |
syIs300 [15xUAS::(delta)pes-10::GFP::unc-54 3'UTR + ttx-3p::RFP + pBlueScript]. GFP cGAL effector. Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with transgenetic marker in next generation. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148. |
| PS6872 |
syIs302 III. |
syIs302 [15xUAS::Δpes-10::GFP::unc-54 3'UTR + ttx-3p::RFP + pBlueScript]. GFP cGAL effector. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148. |
| PS6974 |
syIs337 III. |
syIs337 [15xUAS::Δpes-10::GFP::let-858 3'UTR + ttx-3p::RFP + pBluescript]. GFP cGAL effector. Weak background fluorescence in some head neurons and the head mesodermal cell. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148. |
| PS7149 |
syIs390 X. |
syIs390 [15xUAS::Δpes-10::GFP::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder(NEB)]. GFP cGAL effector. Weak background fluorescence in some head neurons and the head mesodermal cell. [NOTE: (03/18/2020) a user has reported syIs390 does not map to LG X] Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148. |
| PS7136 |
syIs378 I. |
syIs378 [15xUAS::Δpes-10::mKate2::let-858 3'UTR + unc-122p::GFP + 1kb DNA ladder(NEB)]. mKate2 cGAL effector. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148. |
| PS7185 |
syIs406 IV. |
syIs406 [15xUAS::Δpes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript]. GFP::H2B effector. Very weak background fluorescence in first ring of intestinal cells and posterior intestinal cells. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148. |
| PS7186 |
syIs407 V. |
syIs407
[15xUAS::Δpes-10::GFP::H2B::let-858 3'UTR + ttx-3p::RFP + pBlueScript]. GFP::H2B cGAL effector. Very weak background fluorescence in first ring of intestinal cells and posterior intestinal cells. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148. |
| PS7190 |
syIs409 X. |
syIs409
[15xUAS::Δpes-10::mCherry::H2B::let-858 3'UTR + unc-122p::GFP + pBlueScript]. mCherry::H2B cGAL effector. Very weak background fluorescence in first ring of intestinal cells and posterior intestinal cells. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148. |
| PS7203 |
syIs423 V. |
syIs423
[15xUAS::Δpes-10::GCaMP6s::SL2::mKate2::let-858 3'UTR + myo-2p::NLS::mCherry + 1kb DNA ladder(NEB)]. GCaMP6s cGAL effector. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148. |
| PS7044 |
syIs341 IV. |
syIs341
[15xUAS::Δpes-10::hChR2(Y134R)::YFP::let-858 3'UTR + ttx-3p::RFP + pBlueScript]. channelrhodopsin cGAL effector. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148. |
| PS7045 |
syIs342 II. |
syIs342
[15xUAS::Δpes-10::hChR2(Y134R)::YFP::let-858 3'UTR + ttx-3p::RFP + pBlueScript]. channelrhodopsin cGAL effector. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148. |
| PS7199 |
syIs371 III. |
syIs371
[15xUAS::Δpes-10::HisCl1::SL2::GFP::let-858 3'UTR + unc-122p::GFP + 1kb DNA ladder(NEB)]. Histamine chloride channel cGAL effector. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148. |
| PS7107 |
syIs373 I. |
syIs373
[15xUAS::Δpes-10::HisCl1::SL2::GFP::let-858 3'UTR + unc-122p::GFP + 1kb DNA ladder(NEB)]. Histamine chloride channel cGAL. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148. |
| PS7108 |
syIs374 V. |
syIs374
[15xUAS::(delta)pes-10::HisCl1::SL2::GFP::let-858 3'UTR + unc-122p::GFP + 1kb DNA ladder(NEB)]. histamine chloride channel cGAL effector. Reference: Wang H, et al. Nat Methods. 2017 Feb;14(2):145-148. |