Laboratory Information

NameXE View on WormBase
Allele designationwp
HeadMarc Hammarlund
InstitutionYale University
Address BCMM 439
295 Congress Ave
New Haven 06519-1418
United States
Gene classes arch  rtcb  tsen 

Strains contributed by this laboratory

Strain Genotype Species Description
PHX3311 casy-1(syb3311[casy-1::gfp11x7]) II. C. elegans Split-GFP tag inserted into endogenous casy-1 locus by SunyBiotech using CRISPR/Cas9 with two guide RNAs simultaneously. Reference: Ding C, et al. Elife. 2022 Mar 14;11:e73557. PMID: 35285800.
XE1002 unc-70(s1502) V; oxIs12 X. C. elegans oxIs12 [unc-47p::GFP + lin-15(+)]. Unc. Reference: Hammarlund M, et al. J Cell Biol. 2000 May 15;149(4):931-42.
XE1007 oxIs12 X. C. elegans oxIs12 [unc-47p::GFP + lin-15(+)] X. Reference: Byrne AB, et al. Neuron. 2014; 81(3):561-73.
XE1024 daf-2(e1370) III; oxIs12 X. C. elegans oxIs12 [unc-47p::GFP] X. GFP expression in GABA neurons. Higher axon regeneration in aged animals. Reference: Byrne AB, et al. Neuron. 2014; 81(3):561-73.
XE1026 sup-17(n316); oxIs12. C. elegans oxIs12 [unc-47p::GFP] X. GFP expression in GABA neurons. Neuron. 2012 Jan 26;73(2):268-78.
XE1037 geIs3 I; dpy-11(e224) V; oxIs12 X. C. elegans geIs3 [sir-2.1(+) + rol-6(su1006)] I. oxIs12 [unc-47p::GFP] X. GFP expression in GABA neurons. Rollers. Dpy. Reference: Byrne AB, et al. Neuron. 2014; 81(3):561-73.
XE1057 eri-1(mg366) IV; rde-1(ne219) V; lin-15B(n744) X. C. elegans Reference: Firnhaber C & Hammarlund M. PLoS Genet. 2013 Nov;9(11):e1003921.
XE1065 sup-17(n316) I; oxIs12 X ; wpEx16. C. elegans oxIs12 [unc-47p::GFP] X. GFP expression in GABA neurons. wpEx16 [unc-47p::sup-17 + myo-2p::GFP]. Pick animals with GFP+ pharynx to maintain array. GABAergic expression of SUP-17 from wpEx16 rescues sup-17(n316). Reference: El Bejjani R, Hammarlund M. Neuron. 2012; 73(2):268-78.
XE1089 sup-17(n316) I; oxIs12 X; wpEx22. C. elegans oxIs12 [unc-47p::GFP] X. GFP expression in GABA neurons. wpEx22 [myo-3p::sup-17 + myo-2p::GFP]. Pick animals with GFP+ pharynx to maintain array. Muscular expression of SUP-17 from wpEx22 rescues sup-17(n316). Reference: El Bejjani R, Hammarlund M. Neuron. 2012; 73(2):268-78.
XE1090 sup-17(n316) I; oxIs12 X; wpEx30. C. elegans oxIs12 [unc-47p::GFP] X. GFP expression in GABA neurons. wpEx30 [pdi-2p::sup-17 + myo-2p::GFP]. Pick animals with GFP+ pharynx to maintain array. Hypodermal expression of SUP-17 from wpEx30 rescues sup-17(n316). Reference: El Bejjani R, Hammarlund M. Neuron. 2012; 73(2):268-78.
XE1108 daf-16(mu86) I; oxIs12 X. C. elegans oxIs12 [unc-47p::GFP + lin-15(+)] X. Dauer defective. Short lived. Reference: Byrne AB, et al. Neuron. 2014; 81(3):561-73.
XE1109 daf-16(mu86) I; daf-2(e1370) III; oxIs12 X. C. elegans oxIs12 [unc-47p::GFP + lin-15(+)] X. Reference: Byrne AB, et al. Neuron. 2014; 81(3):561-73.
XE1142 wpIs14 X. C elegans wpIs14 [unc-17p::KillerRed + unc-122p::GFP] X. X-Linked based on out-cross data. KillerRed expression in acetylcholine neurons. KillerRed is a red fluorescent protein and photosensitizer that efficiently generates reactive oxygen species (ROS) when activated by light. Reference: Williams DC, et al. Cell Rep. 2013 Oct 31;5(2):553-63.
XE1150 wpIs15 X. C. elegans wpIs15 [unc-47p::KillerRed] X. Slight Unc. KillerRed is a red fluorescent protein and photosensitizer that efficiently generates reactive oxygen species (ROS) when activated by light. wpIs15 produces a Shrinker phenotype after illumination by white light for 2 hrs. Reference: Williams DC, et al. Cell Rep. 2013 Oct 31;5(2):553-63.
XE1158 juIs76 II; wpIs15 X. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. GFP expression in GABAergic motor neurons. wpIs15 [unc-47p::KillerRed] X. Slight Unc. KillerRed is a red fluorescent protein and photosensitizer that efficiently generates reactive oxygen species (ROS) when activated by light. wpIs15 produces a Shrinker phenotype after illumination by white light for 2 hrs. Reference: Williams DC, et al. Cell Rep. 2013 Oct 31;5(2):553-63.
XE1220 wpEx76. C elegans wpEx76 [myo-3p::tom20::tdKillerRed + unc-122p::GFP]. Pick GFP+ to maintain. KillerRed is a red fluorescent protein and photosensitizer that efficiently generates reactive oxygen species (ROS) when activated by light. wpEx76 carries a tandem dimer version of KillerRed targeted to the mitochondria by the addition of a tom-20 targeting sequence (mito-tdKillerRed). Reference: Williams DC, et al. Cell Rep. 2013 Oct 31;5(2):553-63.
XE1235 juIs76 II; slt-1(eh15) X. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Reference: Edwards TJ & Hammarlund M. Cell reports. 2014; 8(1):272-83.
XE1236 juIs76 II; sdn-1(ok449) X. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Reference: Edwards TJ & Hammarlund M. Cell reports. 2014; 8(1):272-83.
XE1269 juIs76 II; rib-2(gk318) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Homozygous viable or sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP gk318 homozygotes (often sterile, lays eggs that don't hatch; some eggs hatch and develop to fertile adulthood). Homozygous hT2 [bli-4 let-? qIs48] inviable. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Pick WT with pharyngeal GFP and check for correct segregation of progeny to maintain. Reference: Edwards TJ & Hammarlund M. Cell reports. 2014; 8(1):272-83.
XE1311 mkk-4(ju91) X; vtIs7. C. elegans vtIs7 [dat-1p::GFP]. GFP expression in dopaminergic neurons. This strain was generated by crossing the mkk-4(jk91) mutation into the vtIs7 background. Reference: Gonzalez-Hunt CP, et al. PLoS One. 2014 Dec 8;9(12):e114459.
XE1347 wpIs39 X. C.elegans wpIs39 [unc-47p:mCherry] X. mCherry expression in GABA neurons. Reference: Byrne AB, et al. Elife. 2016 Oct 4;5. pii: e12734.
XE1375 wpIs36 I; wpSi1 II; eri-1(mg366) IV; rde-1(ne219) V; lin-15B(n744) X. C. elegans wpIs36 [unc-47p::mCherry] I. wpSi1 [unc-47p::rde-1::SL2::sid-1 + Cbr-unc-119(+)] II. Maintain at 15-20C, sterile at 25C. GABAergic neuron-specific RNAi strain. Sensitivity to feeding RNAi is limited to the GABAergic neurons; all other tissues are resistant to RNAi. Superficially wild type with mCherry fluorescence in the GABA motor neurons. Reference: Firnhaber C & Hammarlund M. PLoS Genet. 2013 Nov;9(11):e1003921.
XE1464 daf-16(mu86) I; daf-2(e1370) III; wpSi9. C. elegans wpSi9 [spl-1p::GFP::daf-16A + Cbr-unc-119(+)]. Reference: Byrne AB, et al. Neuron. 2014; 81(3):561-73.
XE1474 wpSi6 II; eri-1(mg366) IV; rde-1(ne219) V; lin-15B(n744) X. C. elegans wpSi6 [dat-1p::rde-1::SL2::sid-1 + Cbr-unc-119(+)] II. Maintain at 15-20C, sterile at 25C. Superficially wild-type. Dopaminergic neuron-specific RNAi strain. Sensitivity to feeding RNAi is limited to the dopaminergic neurons; all other tissues resistant. Reference: Firnhaber C & Hammarlund M. PLoS Genet. 2013 Nov;9(11):e1003921.
XE1481 juIs76 II; sdn-1(ev697) X. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Reference: Edwards TJ & Hammarlund M. Cell reports. 2014; 8(1):272-83.
XE1489 wpEx146. C. elegans wpEx146 [dat-1p::MYR::tdKillerRed + dat-1p::GFP]. Pick GFP+ to maintain. KillerRed expression in acetylcholine neurons. KillerRed is a red fluorescent protein and photosensitizer that efficiently generates reactive oxygen species (ROS) when activated by light. wpEx146 carries a tandem dimer version of KillerRed targeted to the plasma membrane through the addition of a myristoylation tag (mry-tdKillerRed). Reference: Williams DC, et al. Cell Rep. 2013 Oct 31;5(2):553-63.
XE1511 eat-2(ad465) II; oxIs12 X. C. elegans oxIs12 [unc-47p::GFP + lin-15(+)] X. Abnormal feeding. Slow, regular pumping. Reference: Byrne AB, et al. Neuron. 2014; 81(3):561-73.
XE1520 daf-16(mu86) I; daf-2(e1370) III; oxIs12 X; muEx268. C. elegans oxIs12 [unc-47p::GFP + lin-15(+)] X. muEx268 [ges-1p::GFP::daf-16(cDNA) + odr-1::RFP]. Pick RFP+ to maintain. Reference: Byrne AB, et al. Neuron. 2014; 81(3):561-73.
XE1527 daf-16(mu86) I; daf-2(e1370) III; oxIs12 X; wpEx169. C. elegans oxIs12 [unc-47p::GFP + lin-15(+)] X. wpEx169 [rgef-1p::daf-16A + rgef-1p::daf-16F + myo-2p::GFP]. Pick pharyngeal GFP+ to maintain. Reference: Byrne AB, et al. Neuron. 2014; 81(3):561-73.
XE1528 daf-2(e1370ts); daf-16(mu86); oxIs12[Punc-47:GFP, lin-15+]; wpEx170[Prgef-1::daf-16A + Prgef-1::daf-16F + Pmyo-2::GFP], C. elegans daf-2(e1370ts); daf-16(mu86); oxIs12[Punc-47:GFP, lin-15+]; wpEx170[Prgef-1::daf-16A + Prgef-1::daf-16F + Pmyo-2::GFP],
XE1529 daf-2(e1370ts); daf-16(mu86); oxIs12[Punc-47:G- FP, lin-15+]; wpEx171[Pspl-1::daf-16A + Pspl-1::daf-16F + Pmyo-2::GFP], C. elegans daf-2(e1370ts); daf-16(mu86); oxIs12[Punc-47:G- FP, lin-15+]; wpEx171[Pspl-1::daf-16A + Pspl-1::daf-16F + Pmyo-2::GFP],
XE1530 daf-2(e1370ts); daf-16(mu86); oxIs12[Punc-47:GFP, lin-15+]; wpEx172[Pspl-1::daf-16A + Pspl-1::daf-16F + Pmyo-2::GFP], C.elegans daf-2(e1370ts); daf-16(mu86); oxIs12[Punc-47:GFP, lin-15+]; wpEx172[Pspl-1::daf-16A + Pspl-1::daf-16F + Pmyo-2::GFP],
XE1550 wpIs59. C elegans wpIs59 [unc-47p::MYR::tdKillerRed]. KillerRed is a red fluorescent protein and photosensitizer that efficiently generates reactive oxygen species (ROS) when activated by light. wpIs59 carries a tandem dimer version of KillerRed targeted to the plasma membrane through the addition of a myristoylation tag (mry-tdKillerRed). Reference: Williams DC, et al. Cell Rep. 2013 Oct 31;5(2):553-63.
XE1551 wpIs39 X; wpIs9. C. elegans wpIs39 [unc-47p:mCherry] X. wpIs9 [unc-47p::DLK-1::mini-GFP + ccGFP]. mCherry expression in GABA neurons and GFP expression in coelomocytes. Over-expression of dlk-1. Reference: Byrne AB, et al. Elife. 2016 Oct 4;5. pii: e12734.
XE1552 pmk-3(ok169) IV; wpIs39 X; wpIs9. C. elegans wpIs39 [unc-47p:mCherry] X. wpIs9 [unc-47p::DLK-1::mini-GFP + ccGFP]. mCherry expression in GABA neurons and GFP expression in coelomocytes. Over-expression of dlk-1. Reference: Byrne AB, et al. Elife. 2016 Oct 4;5. pii: e12734.
XE1559 daf-18(mg198) IV; oxIs12 X. C. elegans oxIs12 [unc-47p::GFP + lin-15(+)] X. Reference: Byrne AB, et al. Neuron. 2014; 81(3):561-73.
XE1581 wpSi10 II; eri-1(mg366) IV; rde-1(ne219) V; lin-15B(n744) X. C. elegans wpSi10 [unc-17p::rde-1::SL2::sid-1 + Cbr-unc-119(+)] II. Maintain at 15-20C, sterile at 25C. Superficially wild-type. Cholinergic neuron-specific RNAi strain. Sensitivity to feeding RNAi is limited to the cholinergic neurons; all other tissues resistant. Reference: Firnhaber C & Hammarlund M. PLoS Genet. 2013 Nov;9(11):e1003921.
XE1582 wpSi11 II; eri-1(mg366) IV; rde-1(ne219) V; lin-15B(n744) X. C. elegans wpSi11 [eat-4p::rde-1::SL2::sid-1 + Cbr-unc-119(+)] II. Maintain at 15-20C, sterile at 25C. Superficially wild-type. Glutamatergic neuron-specific RNAi strain. Sensitivity to feeding RNAi is limited to the glutamatergic neurons; all other tissues resistant. Reference: Firnhaber C & Hammarlund M. PLoS Genet. 2013 Nov;9(11):e1003921.
XE1583 wpIs36 I; wpSi1 II; eri-1(mg366) IV; rde-1(ne219) V; lin-15B(n744) X; wpEx180. C. elegans wpIs36 [unc-47p::mCherry] I. wpSi1 [unc-47p::rde-1::SL2::sid-1 + Cbr-unc-119(+)] II. wpEx180 [sur-5p::sur-5::GFP::NLS]. Derived by micro-injection of of XE1375 with sur-5p::GFP transgene. Pick GFP+ to maintain. Reference: Firnhaber C, & Hammarlund M. PLoS Genet. 2013 Nov;9(11):e1003921.
XE1593 daf-16(mu86) I; wpSi14 II; daf-2(e1370) III. C. elegans wpSi14 [rgef-1p::GFP::daf-16A + Cbr-unc-119(+)] II. Reference: Byrne AB, et al. Neuron. 2014 Feb 5;81(3):561-73. doi: 10.1016/j.neuron.2013.11.019. PMID: 24440228
XE1677 sqv-5(n3611) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); juIs76 II. C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Homozygous viable or sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP n3611 homozygotes (Sqv Mel). Homozygous hT2 [bli-4 let-? qIs48] inviable. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Pick WT with pharyngeal GFP and check for correct segregation of progeny to maintain. Reference: Edwards TJ & Hammarlund M. Cell reports. 2014; 8(1):272-83.
XE1680 juIs76 II; sdn-1(zh20) slt-1(eh15) X. C.elegans juIs76 [unc-25p::GFP + lin-15(+)] II. Reference: Edwards TJ & Hammarlund M. Cell reports. 2014; 8(1):272-83.
XE1873 aex-6(sa24) I; oxIs12 X. C. elegans oxIs12 [unc-47p::GFP] X. GFP expression in GABA neurons. aex-6 a.k.a. rab-27. Reference: Sekine Y, et al. Cell reports. 2018; 23(2):415-428.
XE1890 aex-6(sa24) I; wpEx287. C.elegans wpEx287 [unc-47p::aex-6::unc-54 3'UTR + myo-2p::mCherry]. Pick mCherry+ to maintain. aex-6 a.k.a. rab-27. Reference: Sekine Y, et al. Cell reports. 2018; 23(2):415-428.
XE1892 wpEx287. C. elegans wpEx287 [unc-47p::aex-6::unc-54 3'UTR + myo-2p::mCherry]. Pick mCherry+ to maintain. aex-6 a.k.a. rab-27. Reference: Sekine Y, et al. Cell reports. 2018; 23(2):415-428.
XE1910 aex-6(sa699) I; oxIs12 X. C. elegans oxIs12 [unc-47p::GFP] X. GFP expression in GABA neurons. High regeneration. aex-6 a.k.a. rab-27. Reference: Sekine Y, et al. Cell reports. 2018; 23(2):415-428.
XE1931 ric-7(n2657) V; wpIs101. C. elegans wpIs101 [itr-1pB::GFP:::rab-3::SL2::mCherry + myo-2p::mCherry]. DA9 and its presynapses labeled. ric-7 causes defective expulsion during defecation and slightly slow growth.  Reference: Ding C & Hammarlund M. Elife. 2018 Oct 29;7. pii: e38829. doi: 10.7554/eLife.38829.
XE1935 wpIs98 I. C. elegans wpIs98 [itr-1pB::Chrimson::SL2::mCherry + odr-1p::RFP] I. Chrimson is expressed in DA9. Use 100 uM ATR in OP50 for optogenetic experiments. Reference: Ding C & Hammarlund M. Elife. 2018 Oct 29;7. pii: e38829. doi: 10.7554/eLife.38829.
XE1995 wpIs98 I; ric-7(n2657) V; wpIs103. C. elegans wpIs98 [itr-1pB::Chrimson::SL2::mCherry + odr-1p::RFP] I. wpIs103 [myo-3p::GCaMP6 + myo-2p::mCherry].  ric-7 causes defective expulsion during defecation and slightly slow growth. Chrimson is expressed in DA9. GCaMP6 expressed in body wall muscles. Use 100 uM ATR in OP50 for optogenetic experiments. Reference: Ding C & Hammarlund M. Elife. 2018 Oct 29;7. pii: e38829. doi: 10.7554/eLife.38829.
XE2043 wpIs98 unc-13(e51) I; ric-7(n2657) V. C. elegans wpIs98 [itr-1pB::Chrimson::SL2::mCherry + odr-1p::RFP] I. Reference: Ding C & Hammarlund M. Elife. 2018 Oct 29;7. pii: e38829. doi: 10.7554/eLife.38829.
XE2045 ric-7(n2657) V; ufIs92; wpIs109. C. elegans ufIs92 [unc-47p::acr-12::GFP].  wpIs109 [itr-1pB::mCherry + odr-1p::RFP]. ric-7 causes defective expulsion during defecation and slightly slow growth. ACR-12::GFP expression in GABA neurons. DA9 is labeled with mCherry. Reference: Ding C & Hammarlund M. Elife. 2018 Oct 29;7. pii: e38829. doi: 10.7554/eLife.38829.
XE2260 casy-1(wp78) II. C. elegans wp78 is a CRISPR/Cas9-engineered 11.5 kb deletion removing the entire coding region of casy-1, the C. elegans homolog of calsyntenin. casy-1(wp78) phenocopies the casy-1(wp60) point mutation and strongly suppresses PVQ degeneration in both ric-7(n2657) and miro-1(wy50180); mtx-2(wy50266) mutants. Reference: Ding C, et al. Elife. 2022 Mar 14;11:e73557. PMID: 35285800.
This laboratory hasn't submitted any alleles to the CGC.