Laboratory Information
| Name | CP View on WormBase |
|---|---|
| Allele designation | nm |
| Head | Haag, Eric |
| Institution | University of Maryland, College Park, MD |
| Address | Bldg 413 Rm 0101J Biology/Biosciences College Park 20742 United States |
| Website | http://www.life.umd.edu/biology/faculty/haag/index.html |
| Gene classes | msrp mss |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| CP101 | Cbr-puf-2(nm66)/Cbr-dpy-?(nm4) II. | C. briggsae | Larval-lethal puf-2 deletion allele. Heterozygotes are WT (slightly Dpy) and segregate 25% Dpy, 50% wild-type heterozygotes, and 25% larval lethal (arrest L1-L2). Maintain by picking WT and checking for correct segregation of progeny. Map distance between nm4 and nm66 has not been preciely determined, but is tight enough that >90% of non-Dpy non-Lva progeny from double-heterozygotes retain the parental genotype. Reference: Liu Q & Haag ES. J Exp Zool. 2013 Part B. |
| CP157 | nmDf1 III. | C. remanei | nmDf1 removes all four tandem paralogs of the mss family (Cre-mss-1, Cre-mss-2, Cre-mss-3, and Cre-mss-4). Male sperm is less competitive than wild-type male sperm, and females have lower brood size due to inbreeding depression. Reference: Yin D, et al. Science. 2018 Jan 5;359(6371):55-61. |
| CP158 | Cre-mss-1(nm74[HA:Cre-mss-1]) III. | C. remanei | The hemaglutinin (HA) epitope tag was inserted using CRISPR/Cas9 through homologous recombination. The nine amino acid HA epitope was placed between C. remanei (EM464) MSS-1 residues 22 and 23, one residue downstream of the predicted mature N-terminus after signal peptide cleavage. NOTE: nm74 was originally described as nmIs9. Derived from parental strain SB146. Reference: Yin D, et al. Science. 2018 Jan 5;359(6371):55-61. |
| CP161 | Cbr-unc-119(nm67) III; nmIs7. | C. briggsae | nmIs7 [Cni-mss-1(+) + Cni-mss-2(+) + Cbr-myo-2::GFP + unc-119(+)]. Insertion site of transgene is not known, but it is not in LG III or X. Males with this transgene are more competitive in siring progeny; also a higher ratio of males in the population. Derived from parental strain CP99, which in turn was derived from AF16. Reference: Yin D, et al. Science. 2018 Jan 5;359(6371):55-61. |
| CP36 | Cbr-fem-2(nm27) III. | C. briggsae | XX animals have no obvious phenotype: self-fertile with normal brood size. XO animals are self-fertile hermaphrodites with low brood size and some somatic gonad defects. Cbr-fem-2/+ XO animals show late-onset germline feminization. AF16 was the parental strain. |
| CP38 | Cbr-tra-1(nm2)/Cbr-let(nm28) III. | C. briggsae | When singled, hermaphrodites should throw 2/3 hermaphrodites and 1/2 nm2 XX males. The lethal appears to balance the nm2 allele pretty well, but precise recombination mapping has not been performed. The XX males maintain their phenotypic resemblance to the unbalanced strain and are probably not fertile due to obvious gonadal deficiencies. This strain has been successfully grown at 15C and 20C. Both strains appear to have complete penetrance of the mutant phenotypes. |
| CP4 | Cbr-dpy-?(nm4) II. | C. briggsae | Dpy. Tightly linked to Cbr-tra-2 on LG II. Molecular identity unknown. Reference: Kelleher DF, et al. Genetics. 2008 Mar;178(3):1415-29. |
| CP87 | Cbr-fem-3(nm63) IV. | C. briggsae | XO are self-fertile hermaphrodites with low brood size and some somatic gonad defects. XX animals have normal brood size. |
| CP89 | Cbr-fem-2(nm27) III; Cbr-fem-3(nm63) IV. | C. briggsae | XX are self-fertile hermaphrodites. |
| CP99 | Cbr-unc-119(nm67) III. | C. briggsae | Derived from AF16. Outcrossed >6x to AF16. Unc, slightly Dpy, no dauer formation (similar to C. elegans unc-119). nm67 is a deletion (827 bp) in Cbr-119 begining in exon 1 and ending 3' of exon 4. Reference: Liu Q, et al. Development. 2012 Apr;139(8):1509-21. |
Alleles contributed by this laboratory
| Allele | Type | DNA Change | Protein Change |
|---|---|---|---|
| nm4 | Allele | ||
| nm3 | Allele | ||
| nm27 | |||
| nm2 | Allele | substitution | nonsense |
| nm63 | Allele | ||
| nm67 | Allele |