Strain Information
| Name | CP196 View On Wormbase |
|---|---|
| Species | C. briggsae |
| Genotype | Cbr-msrp-3(nm85) I; mfIs29. |
| Description | mfIs29 [Cel-lip-1::GFP + Cel-myo-2::GFP]. Strong GFP expression in pharynx and weak GFP expression in various somatic and germline tissues. nm85 is a likely null frameshift mutation in the sperm-expressed Cbr-msrp-3, but has no apparent reproductive phenotypes. To confirm presence of nm85 mutation, use primers AT19+AT20 (WT: 291 nt, nm85: 286 nt). AT19: AAGAAGAGAGAAACCAGAAGC. AT20: AAAAGTAAAACATACCGATCACA. Reference: Van Goor J, et al. Curr Biol. 2025 35:1-7. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x2 |
| Made by | Asan Turdiev |
| Laboratory | CP |
| Reference | Van Goor, J., Turdiev, A., Speir, S.J., Manning, J., and Haag, E.S. (2025). Male secreted short glycoproteins link sperm competition to the reproductive isolation of species. Current Biology 35, 1-7. |
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