Strain Information
| Name | CP198 View On Wormbase |
|---|---|
| Species | C. briggsae |
| Genotype | Cbr-msrp-3(nm77[HA::Cbr-msrp-3]) I. |
| Description | nm77 is an HA epitope tag inserted into the endogenous Cbr-msrp-3 locus, two codons after the predicted signal peptide cleavage site of Cbr-MSRP-3. Anti-HA antibodies recognize the tagged Cbr-MSRP-3 glycoprotein on immunoblots, in the membranous organelles of spermatids, and on the plasma membrane of activated sperm (including pseudopod) in both males and hermaphrodites. To confirm presence of the nm77 insertion, use primers AT19+AT20 (WT: 291 nt, nm77: 318 nt). AT19: AAGAAGAGAGAAACCAGAAGC. AT20: AAAAGTAAAACATACCGATCACA. Reference: Van Goor J, et al. Curr Biol. 2025 35:1-7. |
| Mutagen | CRISPR-Cas9 |
| Outcrossed | x2 |
| Made by | Asan Turdiev |
| Laboratory | CP |
| Reference | Van Goor, J., Turdiev, A., Speir, S.J., Manning, J., and Haag, E.S. (2025). Male secreted short glycoproteins link sperm competition to the reproductive isolation of species. Current Biology 35, 1-7. |
Sign in
or
register an account if you want to order this strain.