Strain Information
| Name | CP217 View On Wormbase |
|---|---|
| Species | C. briggsae |
| Genotype | Cbr-msrp-1(nm86) nmDf3 I; mfIs29. |
| Description | mfIs29 [Cel-lip-1::GFP + Cel-myo-2::GFP]. Strong GFP expression in pharynx and weak GFP expression in various somatic and germline tissues. nmDf3 is an 8411 bp deletion that removes Cbr-msrp-2, Cbr-msrp-3, Cbr-msrp-4, Cbr-msrp-6, and Cbr-msrp-6. Removal of all six Cbr-msrp paralogs has no apparent reproductive phenotypes. [NOTE: Van Goor J, et al. (2005) incorrectly described the nmDf3 as a 5807 bp deletion.] To confirm presence of nm86 mutation, use primers AT130+AT131 (WT: 184 nt, nm86: 224 nt). AT130: CGAAATAATTGAACCTACCAAGA. AT131: CACTCTCTCTGACTGCAAACG. To confirm presence of deletion, use primers AT72+AT73 (WT: 11213 nt, nmDf3: 2802) and AT20+AT75 (WT: 855 nt, nmDf3: no product). AT72: GTACGACGGATAGAGTGTGAT. AT73: CTGTGGGATTATGAAAAGACTC. AT20: AAAAGTAAAACATACCGATCACA. AT75: CAGCAGCAACCTTAGAACAT. Reference: Van Goor J, et al. Curr Biol. 2025 35:1-7. |
| Mutagen | CRISPR-Cas9 |
| Outcrossed | x3 |
| Made by | Asan Turdiev |
| Laboratory | CP |
| Reference | Van Goor, J., Turdiev, A., Speir, S.J., Manning, J., and Haag, E.S. (2025). Male secreted short glycoproteins link sperm competition to the reproductive isolation of species. Current Biology 35, 1-7. |
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