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Strain Species Genotype Add
CB1597 C. elegans unc-103(e1597) III. Show Description
Semidominant. Paralyzed Unc. Prone to spontaneous suppression.
MT2509 C. elegans unc-103(n500) III. Show Description
Semi-dominant Unc.
MT2633 C. elegans unc-103(n500n1211) III. Show Description
Non-Unc.
MT2635 C. elegans unc-103(e1597n1213) III. Show Description
Intragenic revertant.
MT2636 C. elegans unc-103(e1597n1214) III. Show Description
Revertant. WT phenotype.
PS4230 C. elegans unc-103(sy557) III; him-5(e1490) V. Show Description
Semi-dominant. 60% of adult males will have protruding spicules; Prc (protraction constitutive) phenotype. Larval animals and adult hermaphrodites do not display any gross abnormal phenotypes. Prc males have a mating efficiency of 0. Non-Prc males have a mating efficiency of 3. Do not distribute this strain; other labs should request it from the CGC.
CG289 C. elegans unc-103(n1213) pha-1(e2123) III; him-5(e1490) V;rgEx81. Show Description
rgEx81[pha-1(+) + unc-103 promoter E::unc-103 genomic DNA]. Maintain at 20C.
CG490 C. elegans unc-103(n1213) III; egl-2(rg4) him-5(e1490) V; rgEx173. Show Description
rgEx173 [unc-103ep::egl-2(cDNA) + gtl-1p::CFP]. Pick animals with cyan fluorescence in their intestines. rgEx173 rescues food deprivation's ability to suppress unc-103(n1213)-induced male sex muscle seizures.
CG499 C. elegans daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx178. Show Description
rgEx178 [lev-11p::daf-2(+) + lev-11p::GFP]. rgEx178 rescues food deprivation suppression of unc-103(n1213)-induced spicule protraction.
CG500 C. elegans daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx180. Show Description
rgEx180 [aex-3p::daf-2(+) + tnt-4p::GFP]. rgEx180 reduces unc-103(n1213)-induced spicule protraction, and rescues constitutive dauer formation.
CG625 C. elegans unc-103(n1213) pha-1(e2123) III; him-5(e1490) V; rgEx235. Show Description
rgEx235 [plc-3p::YFP + pha-1(+)]. Expresses YFP from a 4.4bk upstream plc-3 promoter. Maintain at 20C.
CG640 C. elegans daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx247. Show Description
rgEx247 [hsp-16p::daf-2(+) + lev-11p::GFP]. rgEx247 restores food-deprivation suppression of unc-103(n1213)-induced spicule protraction.
CG644 C. elegans pha-1(e2123) III; him-5(e1490) V; rgEx251. Show Description
rgEx251[pha-1(+) + osm-12 promoter:: unc-103(gf)]. Maintain at 20C.
VC4911 C. elegans unc-103(gk5979[loxP + Pmyo-2::GFP::unc-54 3' UTR + Prps-27::neoR::unc-54 3' UTR + loxP]) III. Show Description
Homozygous viable. Deletion of 7590 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Please refer to supporting documents linked to the strain name in the CGC Strain Information display. Left flanking sequence: ACGTTTCCGAGTCACTTTGCTCAAACACCC. Right flanking sequence: AGGAAGTGTAGAAATATTGAATGATGATAA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.
LX1918 C. elegans vsIs164 lite-1(ce314) lin-15B&lin-15A(n765) X. Show Description
vsIs164 [unc-103p(E)::GCaMP5 + unc-103p(E)::mCherry + lin-15(+)] X. Integrated transgene using unc-103e promoter to drive GCaMP5 and mCherry expression in vulval muscles; useful for visualizing and quantitating calcium influx in vulval muscle cells. Reference: Collins K, et al. Elife. 2016 Nov 16;5. pii: e21126. doi: 10.7554/eLife.21126.