Caenorhabditis Genetics Center (CGC)

Search Strains

Fewer Fields See WormTagDB for other published tagged loci.
Strain Species Genotype Add
OCF15 C. elegans unc-119(ed3); ocfIs2. Show Description
ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. Stable germline and embryonic expression of ER and NE marker, useful for following NE dynamics through early development. Reference: Joseph-Strauss D, et al. Dev Biol. 2012 May 15;365(2):445-57.
OCF108 C. elegans unc-119(ed3) III; atln-1(ocf102[atln-1::GFP::3xFLAG]) IV; ocfIs2. Show Description
ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. GFP::3xFlag tag inserted at C-terminus of endogenous atln-1 locus. ocf102 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
OCF124 C. elegans unc-119(ed3) III; ItIs33; ocfIs2. Show Description
ItIs33 [pie-1p::GFP::TEV::stag::sas-6 + unc-119(+)]. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
OCF158 C. elegans unc-119(ed3) III; ltIs78; ocfIs2. Show Description
ltIs78 [pie-1p::GFP::TEV::Stag::air-1 spliced coding + unc-119(+)]. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
OCF162 C. elegans ebp-2(or1954[ebp-2::mKate2]) II; unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) ieSi38 IV; ocfIs2. Show Description
ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. mKate2 tag inserted into C-terminus of endogenous ebp2 locus. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
OCF164 C. elegans spd-5(vie26[GFP::spd-5::loxP]) I; unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) ieSi38 IV; ocfIs2. Show Description
ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. GFP tag inserted into N-terminus of endogenous spd-5 locus. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
OCF165 C. elegans spd-5(vie26[GFP::spd-5::loxP]) I; unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) IV; ocfIs2. Show Description
ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. GFP tag inserted into N-terminus of endogenous spd-5 locus. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
OCF167 C. elegans tac-1(or1955[GFP::tac-1]) II; unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) ieSi38 IV; ocfIs2. Show Description
ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. GFP tag inserted into N-terminus of endogenous tac-1 locus. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
OCF172 C. elegans unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2]) III; atln-1(ocf101[atln-1::3xFLAG::AID*]) ieSi38 IV; ltIs78; ocfIs2. Show Description
ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ltIs78 [pie-1p::GFP::TEV::Stag::air-1 spliced coding + unc-119(+)]. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. mTurqoise2 tag with linker sequence inserted into C-terminus of endogenous his-72 locus. GFP::AID* degron tag inserted at C-terminus of endogenous atln-1 locus. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
OCF176 C. elegans spd-5(vie26[GFP::spd-5::loxP]) I; unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2] III; ocfIs2. Show Description
ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. GFP tag inserted at N-terminus of endogenous spd-5 locus. mTurquoise2 tag with linker inserted at C-terminus of endogenous his-72 locus. mCherry::SP12 is a stable germline and embryonic expression of ER and NE marker. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
OCF181 C. elegans unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2] III; ltIs25; ocfIs2. Show Description
ltIs25 [pie-1p::GFP::tba-2 + unc-119(+)]. ItIs25 is a translation fusion of GFP to unspliced genomic tba-2/C47B2.3. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. mTurquoise2 tag with linker inserted at C-terminus of endogenous his-72 locus. mCherry::SP12 is a stable germline and embryonic expression of ER and NE marker. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.
OCF183 C. elegans unc-119(ed3) his-72(erb77[his-72::linker::mTurquoise2] III; ieSi38 IV; atln-1(ocf101[atln-1::3xFLAG::AID*]) IV; ltIs25; ocfIs2. Show Description
ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ltIs25 [pie-1p::GFP::tba-2 + unc-119(+)]. ItIs25 is a translation fusion of GFP to unspliced genomic tba-2/C47B2.3. ocfIs2 [pie-1p:mCherry::sp12::pie-1 3'UTR + unc-119(+)]. mTurquoise2 tag with linker inserted at C-terminus of endogenous his-72 locus. 3xFLAG::AID* tag inserted at C-terminus of endogenous atln-1 locus. mCherry::SP12 is a stable germline and embryonic expression of ER and NE marker. ocf101 guide: CCGCTGATGGACTCAGAAAA. Reference: Maheshwari R, et al. Curr Biol. 2023 Mar 13;33(5):791-806.e7. doi: 10.1016/j.cub.2022.12.059. PMID: 36693370.