Search Strains

maIs188 [mir-228p::GFP + unc-119(+)]. Wild type.

maIs189 [mir-54-56p::GFP + unc-119(+)]. Wild type.

maIs191 [mir-235p::GFP + unc-119(+)]. Wild type.

maIs196 [mir-227-80p::GFP + unc-119(+)]. Wild type.

maIs197 [mir-234p::GFP + unc-119(+)]. Wild type.

maIs206 [mir-81p::GFP + unc-119(+)]. Wild type.

maIs218 [mir-231p::GFP + unc-119(+)]. Wild type.

maIs220 [mir-360p::GFP + unc-119(+)]. Wild type.

maIs227 [mir-90p::GFP + unc-119(+)]. Wild type.

maIs229 [mir-85p::GFP + unc-119(+)]. Wild type.

maIs234 [mir-53::GFP + unc-119(+)]. Wild type.

maIs236 [mir-246p::GFP + unc-119(+)]. Wild type.

maIs251 [mir-1p::GFP + unc-119(+)]. Wild type.

maIs256 [mir-247-797p::GFP + unc-119(+)]. Wild type.

maIs261 [mir-265p::GFP + unc-119(+)] Wild type.

maIs267 [mir-266p::GFP + unc-119(+)]. Wild type.

maIs268 [mir-259p::GFP + unc-119(+)]. Wild type.

maIs276 [mir-60p::GFP + unc-119(+)]. Wild type.

maIs278 [mir-788p::GFP + unc-119(+)]. Wild type.

maIs300 [mir-82p::GFP + unc-119(+)]. Wild type.

maIs347 [mir-793p::GFP + unc-119(+)]. Wild type.

maIs348 [mir-794p::GFP + unc-119(+)]. Wild type.

maIs352 [mir-71p::GFP + unc-119(+)]. Wild type.

maEx246 (cdc-42p::GFP::H2B::cdc-42(mutated) 3`UTR + cdc-42p::mCherry::H2B::cdc-42 3`UTR + pBluescript + pIF9 unc-119(+) + pCFJ150 + pCFJ210). Pick non-Unc to maintain. Reference: Burke SL, Hammell M, Ambros V. Genetics. 2015 Jun 15.

maEx246 (cdc-42p::GFP::H2B::cdc-42(mutated) 3`UTR + cdc-42p::mCherry::H2B::cdc-42 3`UTR + pBluescript + pIF9 unc-119(+) + pCFJ150 + pCFJ210). Pick non-Unc to maintain. Reference: Burke SL, Hammell M, Ambros V. Genetics. 2015 Jun 15.

maEx247 (pat-3p::GFP::H2B::pat-3(mutated) 3`UTR + pat-3p::mCherry::H2B::pat-3 3`UTR + pBluescript + pIF9 unc-119(+) + pCFJ150 + pCFJ210). Pick non-Unc to maintain. DTC migration defects. Reference: Burke SL, Hammell M, Ambros V. Genetics. 2015 Jun 15.

maEx247 (pat-3p::GFP::H2B::pat-3(mutated) 3`UTR + pat-3p::mCherry::H2B::pat-3 3`UTR + pBluescript + pIF9 unc-119(+) + pCFJ150 + pCFJ210). Pick non-Unc to maintain. Reference: Burke SL, Hammell M, Ambros V. Genetics. 2015 Jun 15.

ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Maintain under normal conditions. Reference: Strome et al. (2001) Mol Biol Cell (6):1751-64.

ojIs7 [zyg-12A::GFP + unc-119(+)].

ojIs5 [pie-1p::GFP::dnc-1 + unc-119(+)]

ojIs57 [pie-1::GFP::dnc-2 + unc-119(+)]

ojIs47 [arp-1::GFP + unc-119(+)].

ojIs13 [zyg-12B/C::GFP + unc-119(+)].

ojIs12 [cyk-4::GFP + unc-119(+)]. Strain is not very healthy. Described as integratred array but appears to segregate Uncs. Maintain at 15-20C.

ojEx38 [pie-1::GFP::cmd-1 + unc-119(+)]. Reference: Batchelder et al. (2007) FEBS Lett 581(22):4337-41.

ojIs23 [pie-1p::GFP::C34B2.10].

ojIs31 [pie-1p::spd-3::GFP + unc-119(+)]. ojIs31 rescues spd-3(oj35) lethality at 25 C. Maintain at 25 C. Reference: Dinkelmann MV, et al., Genetics. 2007 Nov;177(3):1609-20.

ojIs34 [GFP::car-1 + unc-119(+)]. N'-tagged GFP::CAR-1 (Y18D10A.17) fusion. Labels P-granules and small cytoplasmic puncta in all cells. Bombardment with pNL1.6::GFP::unc-119 (pfj-1::pie-1 promoter driving GFP::Y18D10A.17 (N-terminal) with unc-119 rescuing fragment).

ojIs35 [pie-1::GFP::rab-11.1 + unc-119(+)].

ojIs37 [pie-1p::GFP::ugtp-1 + unc-119(+)]. Maintain under normal conditions. Reference: Bembenek J, et al. Development. (2007) 134(21):3837-48.

ojIs50 [pie-1p::GFP::air-2 + unc-119(+)]. Wild type looking worms with GFP expression in early embryos. GFP can be seen on stereo microscope in complete darkness with sharp eyes.

ojIs58 [pie-1p::sep-1::GFP + unc-119(+)] III. Maintain under normal conditions. Reference: Bembenek J, et al. Development. (2007) 134(21):3837-48.

ojEx64 [pie-1p::sep-1::GFP + unc-119(+)]. Pick wild-type. Maintain under normal conditions. Reference: Bembenek J, et al. Development. (2007) 134(21):3837-48.

ojIs69 [pie-1::mGFP::chin-1 + unc-119(+)]. N-terminal fusion of mGFP to sole predicted BE0003N10.2 ORF. Reference: Kumfer et al. (2010) Mol Biol Cell (2):266-77.

ojEx83 [pie-1p::mCherry::chin-1a + unc-119(+)]. Maintain by picking wild-type. Check occasionally for maintained mCherry expression.

ruIs38 [myo-2p(partial)::GFP + unc-119(+)] III. Derived by out-crossing AZ218 eleven times to N2.

neEx15 [myo-2::RFP + myo-2::avr-15(+) + unc-119(+)]. MosSCI recipient strain. neEx15 rescues unc-119, making the worms healthier for injection. The array is counter-selectable. Reference: Shirayama M, et al. Cell. 2012 Jul 6;150(1):65-77.

neIs21 [wago-1::3xFLAG + unc-119(+)]. Reference: Gu, et al. 2009 Mol Cell 36:234-44.

neIs20 [pie-1::3xFLAG::csr-1 + unc-119(+)]. Partially rescues sterility (60% dead embryos). Unknown if unc-119(ed3) is still present in the background. Reference: Claycomb J, et al. 2009 Cell 139:123-34.

neIs20 [pie-1::GFP::csr-1 + unc-119(+)]. Partially rescues sterility (60% dead embryos). Unknown if unc-119(ed3) is still present in the background. Reference: Claycomb J, et al. 2009 Cell 139:123-34.