Strain Information
| Name | UJ2015 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | rab-3(miz237[4xwrmScarlet11::rab-3b]) II. |
| Description | 4xwrmScarlet11 tag inserted inserted at 5' end of rab-3b locus using CRISPR/Cas9. Insertion confirmed by sequencing. wrmScarlet11::RAB-3B shows smaller RAB-3 puncta compared to over-expression reporters. The fusion protein is likely non-functional as the strain shows strong aldicarb resistance; however, the localization pattern of 4×wrmScarlet::RAB-3 is similar to that of transgenically expressed RAB-3. 4×wrmScarlet11::rab-3b strain also labels rab-3a; it is possible that 4×wrmScarlet11 inserted in the middle of rab-3a isoform disrupts the functions of RAB-3 in neurotransmission. Reference: Kurashina M, et al. Genetics. 2025 Mar 17;229(3):iyae214. doi: 10.1093/genetics/iyae214. PMID: 39708832. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Mizuki Kurashina |
| Laboratory | UJ |
| Reference | Kurashina M, et al. Genetics. 2025 Mar 17;229(3):iyae214. doi: 10.1093/genetics/iyae214. PMID: 39708832. |
Sign in
or
register an account if you want to order this strain.