Strain Information
| Name | SSM491 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | ubc-9(iow97[3xFLAG::ubc-9]) IV/nT1[qIs51] (IV;V). |
| Description | Heterozygotes are wild-type with pharyngeal-expressed GFP, and segregate wild-type GFP+(pharynx) heterozygotes, arrested nT1[qIs51] homozygotes, and viable non-GFP(pharynx) ubc-9(iow97[3xFLAG::ubc-9]) homozygotes. Maintain the strain by picking wild-type GFP+ worms and checking for correct segregation of progeny. iow97 was created by CRISPR/Cas9 insertion of a 3xflag tag at the N-terminus of the endogenous ubc-9 locus; however, the tagged protein is not fully functional. SSM491 is a replacement for SSM291: analysis shows that in all parameters tested, SSM491 is identical to SSM291, which was genetically unstable and prone to breaking down. Reference: Reichman R, et al. Genetics. 2018 Apr;208(4):1421-1441. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x1 |
| Made by | Emily Koury |
| Laboratory | SSM |
| Reference | Reichman, Shi, Malone and, Smolikove: Genetics. 2018 Apr;208(4):1421-1441. |
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