Caenorhabditis Genetics Center (CGC)

Gene Information: ubc-9

Nameubc-9 View on WormBase
Species C. elegans
SequenceF29B9.6
Genetic positionIV:0.52 +/- 0.016 cM
Genomic positionIV: 4651044..4652447

Strains carrying this gene

Strain Genotype Description
CA649 ubc-9(tm2610) IV/nT1 [unc-?(n754) let-?] (IV;V). Heterozygotes are Unc and segregate Uncs, dead eggs, and Pvul slow growing tm2610 homozygotes. Pick Unc to maintain and check for correct segregation of progeny. Reference: Bhalla N, et al. PLoS Genet. 2008 4(10) e1000235.
SSM491 ubc-9(iow97[3xFLAG::ubc-9]) IV/nT1[qIs51] (IV;V). Heterozygotes are wild-type with pharyngeal-expressed GFP, and segregate wild-type GFP+(pharynx) heterozygotes, arrested nT1[qIs51] homozygotes, and viable non-GFP(pharynx) ubc-9(iow97[3xFLAG::ubc-9]) homozygotes. Maintain the strain by picking wild-type GFP+ worms and checking for correct segregation of progeny. iow97 was created by CRISPR/Cas9 insertion of a 3xflag tag at the N-terminus of the endogenous ubc-9 locus; however, the tagged protein is not fully functional. SSM491 is a replacement for SSM291: analysis shows that in all parameters tested, SSM491 is identical to SSM291, which was genetically unstable and prone to breaking down. Reference: Reichman R, et al. Genetics. 2018 Apr;208(4):1421-1441.