Caenorhabditis Genetics Center (CGC)

Rearrangement Information

NamenT1 View on WormBase
DescriptionRecommended use: General balancing, strain maintenance, mutant screens. reciprocal translocation:right arm of IV and left arm of V; N300 Handling: Easy to manipulate. Heterozygous males mate well. Vulvaless homozygous hermaphrodites completely unable to mate. Cause of vulvaless phenotype unknown. Translocation may break down spontaneously, but analysis of such events is lacking. Heterozygous strains occasionally begin to segregate large numbers of sick-looking progeny while appearing to remain heterozygous, or they occasionally begin to give larger broods (Schein and Baillie, unpublished results). nT1[qIs51] carries an integrated pharyngeal GFP element, and is homozygous inviable; this variant appears to be transferred to male cross progeny more frequently than to hermaphrodite cross progeny. Summary: Translocation, moderately well characterized, very stable. Very effective balancer for right portion of LG IV from right end through unc-17, and for left portion of LG V from left end through unc-76.
Genetic positiongenetic position unknown or not listed
Genomic positiongenomic coordinates unknown or not listed

Strains carrying this rearrangement

Strain Genotype Species Description
AMH50 juIs76; bec-1(ok691) IV/nT1 [qIs51] (IV;V). C. elegans juIs76 [unc-25p::GFP + lin-15(+)] II.  Heterozygotes are wild-type with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-pharyngeal GFP bec-1 homozygotes. Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain.
AP36 mep-1(ok421)/nT1 [qIs51] (IV;V). C. elegans qIs51 [myo-2p::GFP + pes-10p::GFP + F22B7.9p::GFP]. Heterozygotes are wild-type GFP+ and segregate non-GFP ok421 homozygotes, wild-type GFP+ heterozygotes, and arrested nT1[qIs51] aneuploids. Pick wild-type GFP+ and check for correct segregation of progeny to maintain. Received new stock 12/02.
ATD1 unc-119(ed3) III; sqt-3(sc8) par-1(b274) V/nT1[unc-?(n754) let-?] (IV;V); zuIs45 V. C. elegans zuIs45 [nmy-2p::nmy-2::GFP + unc-119(+)] V. Balanced heterozygotes are Unc and segregate Unc (heterozygotes), Rol Par (sqt-3 par-1 homozygotes; maternal effect lethal), and dead eggs (nT1 homozygotes). NMY-2::GFP is expressed in the germline and somatic gonad. Cross of JJ1473 and KK288. Unknown if unc-119(ed3) is still present or homozygous in background. Reference: Small LE & Dawes AT. Mol Biol Cell. 2017 Aug 1;28(16):2220-2231.
AV106 spo-11(ok79) IV/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygotes are Unc and segregate Uncs (heterozygotes), non-Unc spo-11 homozygotes, and dead eggs (nT1 homozygotes). spo-11 homozygotes produce an average of ~200 fertilized eggs but only about 0.1 progeny survive to adulthood. When mated to N2 males, spo-11 homozygotes will produce at least 5-10 cross progeny.
AV112 mre-11(ok179) IV/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygotes are Unc and segregate Uncs (heterozygotes), non-Unc mre-11 homozygotes, and dead eggs (nT1 homozygotes). mre-11 homozygotes produce about 200 fertilized eggs but only about 2-3% of these eggs survive to adulthood (this mutation cannot be maintained in a homozygous condition). Occasionally non-Unc progeny that do not demonstrate the mre-11(ok179) mutant phenotype arise when grown in large liquid cultures. mre-11 is the predicted gene ZC302.1
AV115 msh-5(me23) IV/nT1 [unc-?(n754) let-?] (IV;V). C. elegans Heterozygotes are Unc and segregate Uncs (heterozygotes), non-Unc msh-5 homozygotes, and dead eggs (nT1 homozygotes). msh-5 homozygotes give 97.9% dead eggs; of those that hatch, 42% are male.
AV157 spo-11(me44)/nT1 [unc-?(n754) let-? qIs50] (IV;V). C. elegans Balanced heterozygotes are GFP+ Unc and segregate GFP+ Unc (heterozygotes), non-GFP non-Unc spo-11(me44) homozygotes, and dead eggs (nT1 homozygotes). spo-11(me44) homozygotes are viable and produce more than 90% dead eggs (a large fraction of the survivors are males — strong Him phenotype); cytologically they lack chiasmata in diakinesis-stage oocytes and lack RAD-51 foci. Maintain by picking Unc.
AV271 him-3(me80)/nT1 [unc-?(n754) let-? qIs50] (IV;V). C. elegans Balanced heterozygotes are GFP+ Unc and segregate GFP+ Unc (heterozygotes), non-GFP non-Unc him-3(me80) homozygotes, and dead eggs (nT1 homozygotes). him-3(me80) homozygotes are viable and non-Unc. They produce more than 85% dead eggs and a large fraction (11%) of the survivors are males (Him phenotype). Cytologically they exhibit a reduced level of HIM-3 loading and fewer stretches of SYP-1 than WT. In diakinesis-stage oocytes, they contain a mixture of bivalents and univalents. Maintain by picking Unc.
AV276 syp-2(ok307) V/nT1 [unc-?(n754) let-?(m435)] (IV;V). C. elegans Balanced heterozygotes are Unc and segregate Unc (heterozygotes), non-Unc syp-2(ok307) homozygotes, and dead eggs (nT1 homozygotes). syp-2(ok307) homozygotes are viable and non-Unc. They produce 96% dead eggs and 44% males; cytologically they lack chiasmata in diakinesis-stage oocytes, exhibit persistent polarized nuclear organization during earlier meiotic prophase, lack synaptonemal complexes, and exhibit unstable pairing of homologous chromosomes.
AV307 syp-1(me17) V/nT1 [unc-?(n754) let-? qIs50] (IV;V). C. elegans Balanced heterozygotes are GFP+ Unc and segregate GFP+ Unc (heterozygotes), non-GFP non-Unc syp-1(me17) homozygotes, and dead eggs (nT1 homozygotes). syp-1(me17) homozygotes produce 95% dead embryos and 38% males. Cytologically they lack chiasmata in diakinesis-stage oocytes, exhibit persistent polarized nuclear organization during earlier meiotic prophase, lack synaptonemal complexes, and exhibit unstable pairing of homologous chromosomes. qIs50 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter (F22B7.9) driving GFP in the intestine.
AV473 rad-50(ok197) V/nT1 [qIs51] (IV;V). C. elegans qIs51 [myo-2p::GFP + pes-10p::GFP + F22B7.9p::GFP]. Heterozygotes are wild-type GFP+ and segregate non-GFP ok197 homozygotes (viable, sterile), wild-type GFP+ heterozygotes, and arrested nT1[qIs51] aneuploids. rad-50 homozygotes are viable, produce more than 95% dead eggs and a large fraction of the survivors are male (Him phenotype). Pick wild-type GFP+ and check for correct segregation of progeny to maintain. Reference: Hayashi M, et al. PLoS Genet. 2007 Nov;3(11):e191.
BC1201 sDf19/nT1 IV; +/nT1 V. C. elegans sDf19 has a dominant twitcher phenotype, and is homozygous lethal-usually arresting as embryos, but occasionally will hatch. Heterozygotes are twitchers and segregate twitchers, Vulvaless and dead eggs (or lethal early larvae). This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC1216 sDf21 dpy-4/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC1217 sDf22/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC1227 sDf23/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.
BC1291 let-93(s734) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul, UncLet and dead eggs. Lethal mid-larval. Maintain by picking WT.
BC1548 unc-22(s7) let-67(s214)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul and Twitcher Steriles. The sterility can be rescued by male sperm.
BC1907 let-68(s1081) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul and LetUncTwitcher. Lethal ??. Maintain by picking WT.
BC1912 let-652(s1086) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Egl, dead eggs and UncLets. Lethal early larval. Maintain by picking WT.
BC2001 let-98(s1117) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul, UncLet and dead eggs. Lethal late larval. Maintain by picking WT.
BC2003 let-96(s1112) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul, Lethal Twitchers and dead eggs. Lethal mid-larval. Maintain by picking WT.
BC2008 unc-22(s7) unc-31(e169) let-315(s1101)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul, UncLet and dead eggs. Lethal mid-larval. Maintain by picking WT.
BC2010 let-60(s1124) unc-22(s7) unc-31(e169) IV/nT1 (IV;V). C. elegans Heterozygotes are WT and segregate WT, Vul and UncLethals. Strong allele of let-60. Unc Twitchers arrest as rod-like early larvae; rare escapers die as Vul adults.
BC2013 unc-22(s7) let-97(s1121) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, UncLet (Twitcher), Vul, and dead eggs. Lethal early larval. Maintain by picking WT.
BC2020 let-70(s1132) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul, UncLet (twitchers) and dead eggs. Lethal early larval. Maintain by picking WT. See also WBPaper00002501.
BC2021 let-289(s1133) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vuls, early larval lethals which are Unc and Twitch, and dead eggs. Maintain by picking WT. Not outcrossed!
BC2024 let-291(s1139) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vuls, early larval lethals which are Unc and Twitch, and dead eggs. Maintain by picking WT. Not outcrossed!
BC2029 let-60(s1155) unc-22(s7) unc-31(e169) IV/nT1 (IV;V). C. elegans Heterozygotes are WT and segregate WT, Vul and Twitcher UncLethals. s1155 is viable; 14% Vul.
BC2034 let-292(s1146) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vuls, early larval lethals which are Unc and Twitch, and dead eggs. Maintain by picking WT. Not outcrossed!
BC2035 unc-22(s7) unc-31(e169) let-317(s1153)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Egl, UncLet and dead eggs. Lethal at embryo-early larval. Maintain by picking WT.
BC2036 let-100(s1160) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul, UncLet and dead eggs. Lethal early larval. Maintain by picking WT.
BC2037 let-293(s1166) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are short and somewhat Egl. Offspring are short. Segregate Vuls, early larval lethals which are Unc and Twitch, and dead eggs. Not outcrossed!
BC2047 let-290(s1140) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vuls, early larval lethals which are Unc and Twitch, and dead eggs. Maintain by picking WT. Not outcrossed!
BC2049 let-658(s1149) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, UncLet, Egl and dead eggs. Maintain by picking WT.
BC2053 let-307(s1171) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Egl, Lethal Twitchers and dead eggs. Lethal mid-larval. Maintain by picking WT.
BC2060 let-311(s1195) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul, UncLet and dead eggs. Lethal late larval. Maintain by picking WT.
BC2061 let-69(s1111) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Egl, Lethal Twitchers and dead eggs. Lethal early larval. Maintain by picking WT.
BC2063 unc-22(s7) let-309(s1115) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul, UncLet and dead eggs. Lethal late larval. Maintain by picking WT.
BC2067 let-659(s1152) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, UncLet, Egl and dead eggs. Maintain by picking WT.
BC2069 let-651(s1165) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul, Lethals and dead eggs. Maintain by picking WT.
BC2070 let-294(s1103) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vuls, early larval lethals which are Unc and Twitch, and dead eggs. Maintain by picking WT. Not outcrossed!
BC2079 unc-22(s7) unc-31(e169) let-301(s1134)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul, UncLet, and dead eggs. Lethal early larval. Maintain by picking WT.
BC2088 let-295(s1175) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vuls, early larval lethals which are Unc and Twitch, and dead eggs. Maintain by picking WT. Not outcrossed!
BC2094 unc-22(s7) unc-31(e169) let-313(s1135)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul, Sterile Unc, and dead eggs. Maintain by picking WT.
BC2105 let-308(s1705) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Egl, Lethal Twitchers and dead eggs. Lethal mid-larval. Maintain by picking WT.
BC2107 unc-22(s7) unc-31(e169) let-314(s1206)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Egl, UncLet and dead eggs. Lethal at embryo-early larval. Maintain by picking WT.
BC2113 unc-22(s7) unc-31(e169) let-318(s1218)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul, Sterile Uncs and dead eggs. Maintain by picking WT.
BC2120 unc-22(s7) unc-31(e169) let-321(s1228)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Egl, Sterile Unc, and dead eggs. Maintain by picking WT.
BC2124 unc-22(s7) unc-31(e169) let-322(s1238)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, Vul, UncLets and dead eggs. Lethal mid-larval. Maintain by picking WT.
BC2126 let-657(s1254) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. C. elegans Heterozygotes are WT and segregate WT, UncLet, Vul and dead eggs. Maintain by picking twitchers (hets) in 1% nicotine.