Strain Information
| Name | RAF2181 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | ieSi57 II; daf-2(bch-40[degron::3xFLAG::STOP::SL2::SV40::degron::wrmScarlet::egl-13NLS]) unc-119(ed3) III. |
| Description | ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Degron tag inserted into endogenous daf-2 locus. ieSi57 is a single-copy transgene insertion into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. This strain can be used for auxin-inducible degradation (AID) of target proteins in somatic tissues. Reference: Venz R, et al. Elife. 2021 Sep 10;10:e71335. doi: 10.7554/eLife.71335. PMID: 34505574. |
| Mutagen | No mutagen |
| Outcrossed | x4 |
| Made by | Iskra Katic |
| Laboratory | LSD |
| Reference | Richard Venz, Tina Pekec, Iskra Katic, Rafal Ciosk, Collin Yvès Ewald (2021) End-of-life targeted degradation of DAF-2 insulin/IGF-1 receptor promotes longevity free from growth-related pathologies eLife 10:e71335 https://doi.org/10.7554/eLife.71335 |
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