Strain Information

Name PHX530   View On Wormbase
Species C. elegans
Genotypenlp-11(syb530) II.
DescriptionSuperficially wild-type. syb530 is a 2042 bp deletion of the entire nlp-11 gene. Flanking sequences:tatttctcctattgagtgcaaaaaagagtgaaa-acatcaacaaataaaataccatacca
acgagt
Primers for genotyping: Fwd: gtcctcaccattcccctagg Interal (fwd): TCTGATCGACGCTGGAAAGA Rev: gaataggaagagggcggagg PCR product: WT 264 bp / syb530 -- ; WT 2551 bp / syb530 509 bp. Reference: Konietzka J, et al. Curr Biol. 2020 Jan 6;30(1):1-16.e13. doi: 10.1016/j.cub.2019.10.048. PMID: 31839447
Mutagen
Outcrossedx0
Made bySunyBiotech
Laboratory HBR
Reference Konietzka et al. (2019), Epidermal Growth Factor signaling promotes sleep through a combined series and parallel neural circuit, Current Biology (accepted, but not published yet. DOI will be sent after publication process is finished)
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