Strain Information
| Name | OH17582 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | daf-16(ot853[daf-16::mNG::AID]) I; otSi2 II; daf-2(e1370) III. |
| Description | otSi2 [ges-1p::TIR1(F79G)::mRuby::unc-54 3'UTR + Cbr-unc-119(+) *ieSi61] II. Temperature-senstive daf(c): maintain at 15C. Intestine-specific TIR1 sequence in ieSi61 allele was edited to TIR1(F79G) using CRISPR/Cas9 to make it compatible with AID2. [TCC GTC GAG CTC AAG GGA AAG CCA CAC TTC] edited to [AGT GTC GAA TTG AAG GGA AAG CCA CAC GGA]. This strain can be used to deplete DAF-16 specifically from the intestine with the modified auxin 5-Ph-IAA. Constitutive dauer formation at 25 C due to daf-2(e1370). Reference: Sural S, et al. bioRxiv 2025.01.06.631508; doi: https://doi.org/10.1101/2025.01.06.631508. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Surojit Sural |
| Laboratory | OH |
| Reference | Reference: Sural S, et al. bioRxiv 2025.01.06.631508; doi: https://doi.org/10.1101/2025.01.06.631508. |
Sign in
or
register an account if you want to order this strain.