| CB3610 |
mig-6(e1931)/dpy-11(e224) V. |
Heterozygotes are WT and segregate WT, Dpys, and steriles. mig-6(e1931) hermaphrodites are sterile, with failure of DTC migrations. Gonad sterile, disorganized, otherwise hermaphrodites appear normal. Strain is imperfectly balanced - check for correct segregation of Dpy and Mig from WT hermaphrodites. |
| NK2442 |
mig-6(qy37[mNG+loxP::mig-6]) V. |
Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. mNeonGreen is inserted at the N-terminus right before a putative proprotein convertase cleavage site and Western analysis indicates most of the mNeonGreen is cut from the MIG-1 protein and is diffuse in the extracellular fluid. See Figure S1 in Keeley et al., Dev Cell. 2020 Jul 6;54(1):60-74.e7. doi: 10.1016/j.devcel.2020.05.022. PMID: 32585132 |
| NK2557 |
mig-6(qy73[mig-6::mNG+loxP]) V. |
Superficially wild-type. Specifically tags the long isoform of mig-6. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NW1313 |
mig-6(ev700) V. |
Abnormal distal tip cell migration detectable as clear patches in anterior and posterior. Partially penetrant embryonic arrest and early larval lethal. |
| NW1314 |
mig-6(ev701) V. |
Abnormal distal tip cell migration detectable as clear patches in anterior and posterior. Partially penetrant embryonic arrest and early larval lethal. |
| NW1619 |
dpy-11(e224) mig-6(ev700) V/eT1 (III;V). |
Heterozygotes display abnormal distal tip cell migration detectable as clear patches in anterior and posterior. Heterozygotes segregate WT, Dpy, abnormal DTC migration, Unc, and dead eggs. Maintain by picking wild-type. |
| NW1623 |
dpy-11(e224) mig-6(ev788) V/eT1 (III;V). |
Heterozygotes display partially penetrant (~10%) abnormal distal tip cell migration detectable as clear patches in anterior and posterior. Heterozygotes segregate WT, abnormal DTC migration, Unc, and dead eggs. Maintain by picking wild-type. |
| NW1625 |
dpy-11(e224) mig-6(e1931) V/eT1 (III;V). |
Heterozygotes are wild-type and segregate WT, Dpy-Ste Unc, and dead eggs. Maintain by picking wild-type. |
| NW1675 |
mig-6(oz113) V/nT1 [qIs51] (IV;V). |
Heterozygotes are wild-type and GFP+. Heterozygotes segregate WT GFP+, sterile GFP- homozygotes, and dead eggs. Maintain by picking GFP+ wild-type. |
| QC104 |
etIs2 III; mig-6(et4) V. |
etIs1 [ric-19p::ric-19::GFP + rol-6(su1006). Low levels of ric19::GFP fusion protein expression driven by the ric-19 promoter in all neurons except for strong expression in the pharyngeal M2 neurons. 100% of worms have a twisted pharynx, which is easily seen as a twisted appearance of the M3 neurons. |
| QC133 |
mig-6(sa580) V. |
sa580 is a G-to-A transition that changes a glycine at position 965 to a glutamate in MIG-6. Reference: Jafari M, et al. (2010) Genetics 186:969-982. |