Strain Information
| Name | MCJ217 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | mir-35(cdb2 cdb4) II; egl-1(cdb97) V. |
| Description | Superficially wild type. Seed mutation of mir-35 was made by two rounds of CRISPR/Cas9 editing. cdb2 is a 50 bp deletion of the mir-35 locus. cdb4 was created by successive homology-directed repair of the disrupted mir-35 locus with a protospacer to preserve the secondary structure of the primary and precursor hairpin. egl-1(cdb97) contains engineered mutations in mir-35 binding site in the 3’UTR region making the sequence complementary to the mir-35(cdb4) variant. Reference: Yang B, et al. Genes Dev. 2020 Sep 1;34(17-18):1227-1238. PMID: 32820039 |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x2 |
| Made by | Bing Yang |
| Laboratory | MCJ |
| Reference | B. Yang, M. Schwartz, K. McJunkin. (2020) In vivo CRISPR screening for phenotypic targets of the mir-35-42 family in C. elegans. Genes & Development 34:1227-1238. |
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