Strain Information
| Name | JWW253 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | wrdSi3 II; ifet-1(dfw16[ifet-1::mScarlet-I::AID*::3xFlag]) III. |
| Description | mScarlet-I::AID*::3xFlag tags inserted at the C-terminus of the endogenous ifet-1 locus. wrdSi3 [sun-1p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Germline and early embryo-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in germline and early embryo nuclei. ifet-1 visualized by mScarlet in oocytes with inducible acute degradation when grown on 4mM auxin. No defect in hermaphrodite embryo viability. Reference: Bhatia P, et al. Life Sci Alliance. 2025 May 29;8(8):e202503387. doi: 10.26508/lsa.202503387. PMID: 40441896. |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x0 |
| Made by | Jeff Woodruff |
| Laboratory | JWW |
| Reference | Bhatia P, Tafur J, Amin R, Familiari NE, Yaguchi K, Tran VM, Bond A, Bukulmez O, Woodruff JB. Condensate-forming eIF4ET ensures adequate levels of meiotic proteins to support oocyte storage. Life Sci Alliance. 2025 May 29;8(8):e202503387. doi: 10.26508/lsa.202503387. PMID: 40441896; PMCID: PMC12122253. |
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