Strain Information
| Name | JK6541 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | gld-1(q1243) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). |
| Description | Pick GFP+ to maintain. CRIPSR-engineered modification of gld-1 FBEa and FBEa* in gld-1 3'UTR. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q1243 homozygotes (sterility/reduced fertility). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Increase in distal GLD-1 protein levels and decrease in proximal GLD-1 protein levels. Qiu et al., in preparation. |
| Mutagen | Crispr/Cas9 |
| Made by | Sarah Crittenden |
| Laboratory | JK |
| Reference | Qiu et al., in preparation |
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