Strain Information

Name JK6099   View On Wormbase
Species C. elegans
Genotypelag-2(q1049[lag-2::3xV5]) V.
Description3xV5 tag inserted at C-terminus of endogenous lag-2 locus. Hermaphrodite DTC stain well. Animals are fertile and look wild-type. tracr + crRNA targeting the C-terminus of LAG-2 was injected with Cas-9 protein and repair template to insert a 3xV5 tag, following Fire and Seydoux lab RNP co-CRISPR strategy (with unc-58 crRNA and repair oligo to mark edited offspring).
Made bySarah Crittenden
Laboratory JK
Reference none
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