Strain Information
| Name | JK6099 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | lag-2(q1049[lag-2::3xV5]) V. |
| Description | 3xV5 tag inserted at C-terminus of endogenous lag-2 locus. Hermaphrodite DTC stain well. Animals are fertile and look wild-type. tracr + crRNA targeting the C-terminus of LAG-2 was injected with Cas-9 protein and repair template to insert a 3xV5 tag, following Fire and Seydoux lab RNP co-CRISPR strategy (with unc-58 crRNA and repair oligo to mark edited offspring). |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x2 |
| Made by | Sarah Crittenden |
| Laboratory | JK |
| Reference | none |
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