Strain Information
| Name | JK6059 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | glp-1(q1035[*q1000]) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). |
| Description | Pick GFP+ to maintain. CRISPR-engineered RAM mutations in endogenous glp-1 locus with C-terminal 4xV5 tag. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q1035 homozygotes (sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Derived by modification of parental strain JK5933 glp-1(q1000). |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x2 |
| Made by | Sarah Crittenden |
| Laboratory | JK |
| Reference | unpublished |
Sign in
or
register an account if you want to order this strain.