Strain Information
| Name | JK5796 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | lst-1(q869) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). |
| Description | Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q869 homozygotes (viable and somewhat fertile). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. q869 is a deletion of the entire lst-1 coding sequence as well as 139 bp upstream of M71 start codon and 228 bp downstream of the coding sequence. Reference: Haupt KA, et al. Development. 2019 Oct 17;146(20):dev181644. doi: 10.1242/dev.181644. PMID: 31515205 |
| Mutagen | Crispr/Cas9 |
| Outcrossed | x7 |
| Made by | Kim Haupt |
| Laboratory | JK |
| Reference | Haupt KA, Enright AL, Ferdous AS, Kershner A, Shin H, Wickens M, and Kimble J. (2019) The molecular basis of LST-1 self-renewal activity and its control of stem cell pool size. Development, 146(20). [PDF] [PubMed] |
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