Strain Information
| Name | JK4307 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | rnp-8(tm2435) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). |
| Description | Heterozygotes are WT and GFP+. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal. Do not distribute this strain; other labs should request it directly from the CGC. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. PCR using 3 primers: primer A: TCA GCC GAC AAT CTC CGA; primer B: GTA CTG ATT GTA TCC ACC GGC; primer C: GCT CAT AGA AAA GCG ATG G. WT band = 0.5 kb; heterozygote bands = 0.8 and 0.5 kb (double bands), tm2435 bands = 0.8 kb. |
| Mutagen | |
| Outcrossed | x13 |
| Made by | KW Kim |
| Laboratory | JK |
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