Strain Information
Name | JK4307 View On Wormbase |
---|---|
Species | C. elegans |
Genotype | rnp-8(tm2435) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). |
Description | Heterozygotes are WT and GFP+. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. PCR using 3 primers: primer A: TCA GCC GAC AAT CTC CGA; primer B: GTA CTG ATT GTA TCC ACC GGC; primer C: GCT CAT AGA AAA GCG ATG G. WT band = 0.5 kb; heterozygote bands = 0.8 and 0.5 kb (double bands), tm2435 bands = 0.8 kb. |
Mutagen | No mutagen |
Outcrossed | x13 |
Made by | KW Kim |
Laboratory | JK |
Sign in
or
register an account if you want to order this strain.