Strain Information
| Name | JDW877 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | wrt-2(wrd365[linker::mNG::3xFLAG::linker (internal)]) X |
| Description | Modular linker::mNeonGreen::3xFLAG::linker tag inserted internally in exon 1 in the endogenous col-75 locus by CRISPR; produces a translation fusion after amino acid 18, immediately following the signal peptide.. Allele obtained using plasmid-based unc-119 selection in a temperature-sensitive unc-119(kst33) III background The unc-119(+) cassette was removed through Cre-mediated excision and the unc-119(kst33) allele was removed by outcrossing. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. |
| Mutagen | CRISPR |
| Outcrossed | x0 |
| Made by | Jordan Ward |
| Laboratory | JDW |
| Reference | Unpublished |
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